Characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatments
BackgroundA comprehensive peripheral immune cell characterization including novel immunosuppressive subsets myeloid-derived suppressive cells (MDSCs) in kidney transplant recipients (KTRs) under different immunosuppressive treatments can help: 1) Immunosuppression situation and allograft acceptance...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1605664/full |
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| author | Yunze Tai Nanjing Li Jiwen Fan Haohan Zhang Honghui Long Lin Yan Weihua Feng Junlong Zhang Bei Cai Yu Fan Yao Luo Yi Li |
| author_facet | Yunze Tai Nanjing Li Jiwen Fan Haohan Zhang Honghui Long Lin Yan Weihua Feng Junlong Zhang Bei Cai Yu Fan Yao Luo Yi Li |
| author_sort | Yunze Tai |
| collection | DOAJ |
| description | BackgroundA comprehensive peripheral immune cell characterization including novel immunosuppressive subsets myeloid-derived suppressive cells (MDSCs) in kidney transplant recipients (KTRs) under different immunosuppressive treatments can help: 1) Immunosuppression situation and allograft acceptance assessment; 2) Infection and rejection emergence indication; 3) Beneficial immunosuppressive regimens’ selection.Methods26 KTRs with an average transplant duration of 360 days and 13 healthy controls were enrolled in this study. 11KTRs were included in the SRL-based therapy group and the other 15 in the TAC-based therapy group. Flow cytometry was used to detect the percentages and absolute numbers of MDSCs, T cell populations, HLA-DR+ monocytes, neutrophil CD64 index, and cytokines in peripheral blood.ResultsIn KTRs, the expression of G-MDSCs and M-MDSCs was significantly higher than the HCs, while the expression of HLA-DR+ monocytes, CD38+/CD28+ activated T cells, CD4+ naïve T cells, CD4+ effector memory T cells, and central memory T cells were significantly lower. The use of mTOR inhibitors in KTRs induced changes in the distribution of activated and naïve-memory T cell subsets and decreased proinflammatory cytokines.DiscussionIn KTRs, G-MDSCs and M-MDSCs accumulated while functionally activated, naïve-memory T cell populations and HLA-DR+ monocytes markedly decreased one year after transplantation. Additionally, the number of MDSCs and T cell subsets following transplantation is likely regulated by mTOR inhibitors. |
| format | Article |
| id | doaj-art-1782634c7cb9422da0df6b2e7b5fb0a5 |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-1782634c7cb9422da0df6b2e7b5fb0a52025-08-20T03:10:04ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-06-011610.3389/fimmu.2025.16056641605664Characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatmentsYunze Tai0Nanjing Li1Jiwen Fan2Haohan Zhang3Honghui Long4Lin Yan5Weihua Feng6Junlong Zhang7Bei Cai8Yu Fan9Yao Luo10Yi Li11Department of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDivision of Radiotherapy, Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Transfusion Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaBackgroundA comprehensive peripheral immune cell characterization including novel immunosuppressive subsets myeloid-derived suppressive cells (MDSCs) in kidney transplant recipients (KTRs) under different immunosuppressive treatments can help: 1) Immunosuppression situation and allograft acceptance assessment; 2) Infection and rejection emergence indication; 3) Beneficial immunosuppressive regimens’ selection.Methods26 KTRs with an average transplant duration of 360 days and 13 healthy controls were enrolled in this study. 11KTRs were included in the SRL-based therapy group and the other 15 in the TAC-based therapy group. Flow cytometry was used to detect the percentages and absolute numbers of MDSCs, T cell populations, HLA-DR+ monocytes, neutrophil CD64 index, and cytokines in peripheral blood.ResultsIn KTRs, the expression of G-MDSCs and M-MDSCs was significantly higher than the HCs, while the expression of HLA-DR+ monocytes, CD38+/CD28+ activated T cells, CD4+ naïve T cells, CD4+ effector memory T cells, and central memory T cells were significantly lower. The use of mTOR inhibitors in KTRs induced changes in the distribution of activated and naïve-memory T cell subsets and decreased proinflammatory cytokines.DiscussionIn KTRs, G-MDSCs and M-MDSCs accumulated while functionally activated, naïve-memory T cell populations and HLA-DR+ monocytes markedly decreased one year after transplantation. Additionally, the number of MDSCs and T cell subsets following transplantation is likely regulated by mTOR inhibitors.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1605664/fullkidney transplantationimmune cell biomarkermyeloid-derived suppressor cellsmTOR inhibitorsflow cytometry |
| spellingShingle | Yunze Tai Nanjing Li Jiwen Fan Haohan Zhang Honghui Long Lin Yan Weihua Feng Junlong Zhang Bei Cai Yu Fan Yao Luo Yi Li Characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatments Frontiers in Immunology kidney transplantation immune cell biomarker myeloid-derived suppressor cells mTOR inhibitors flow cytometry |
| title | Characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatments |
| title_full | Characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatments |
| title_fullStr | Characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatments |
| title_full_unstemmed | Characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatments |
| title_short | Characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatments |
| title_sort | characterization of peripheral immune cells in kidney transplantation recipients under different immunosuppressive treatments |
| topic | kidney transplantation immune cell biomarker myeloid-derived suppressor cells mTOR inhibitors flow cytometry |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1605664/full |
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