Complex Inflammation mRNA-Related Response in ALS Is Region Dependent
Inflammatory changes are analyzed in the anterior spinal cord and frontal cortex area 8 in typical spinal-predominant ALS cases. Increased numbers of astrocytes and activated microglia are found in the anterior horn of the spinal cord and pyramidal tracts. Significant increased expression of TLR7, C...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2015/573784 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558015468797952 |
|---|---|
| author | Sara Berjaoui Mónica Povedano Paula Garcia-Esparcia Margarita Carmona Ester Aso Isidre Ferrer |
| author_facet | Sara Berjaoui Mónica Povedano Paula Garcia-Esparcia Margarita Carmona Ester Aso Isidre Ferrer |
| author_sort | Sara Berjaoui |
| collection | DOAJ |
| description | Inflammatory changes are analyzed in the anterior spinal cord and frontal cortex area 8 in typical spinal-predominant ALS cases. Increased numbers of astrocytes and activated microglia are found in the anterior horn of the spinal cord and pyramidal tracts. Significant increased expression of TLR7, CTSS, and CTSC mRNA and a trend to increased expression of IL10RA, TGFB1, and TGFB2 are found in the anterior lumbar spinal cord in ALS cases compared to control cases, whereas C1QTNF7 and TNFRSF1A mRNA expression levels are significantly decreased. IL6 is significantly upregulated and IL1B shows a nonsignificant increased expression in frontal cortex area 8 in ALS cases. IL-6 immunoreactivity is found in scattered monocyte-derived macrophages/microglia and TNF-α in a few cells of unknown origin in ALS cases. Increased expression and abnormal distribution of IL-1β occurred in motor neurons of the lumbar spinal cord in ALS. Strong IL-10 immunoreactivity colocalizes with TDP-43-positive inclusions in motor neurons in ALS cases. The present observations show a complex participation of cytokines and mediators of the inflammatory response in ALS consistent with increased proinflammatory cytokines and sequestration of anti-inflammatory IL-10 in affected neurons. |
| format | Article |
| id | doaj-art-17769dee4380482d939ddeeae2abe174 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-17769dee4380482d939ddeeae2abe1742025-02-03T01:33:19ZengWileyNeural Plasticity2090-59041687-54432015-01-01201510.1155/2015/573784573784Complex Inflammation mRNA-Related Response in ALS Is Region DependentSara Berjaoui0Mónica Povedano1Paula Garcia-Esparcia2Margarita Carmona3Ester Aso4Isidre Ferrer5Institute of Neuropathology, Bellvitge University Hospital, University of Barcelona, CIBERNED (Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas), 08907 L’Hospitalet de Llobregat, SpainALS Unit, Service of Neurology, Bellvitge University Hospital, 08907 L’Hospitalet de Llobregat, SpainInstitute of Neuropathology, Bellvitge University Hospital, University of Barcelona, CIBERNED (Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas), 08907 L’Hospitalet de Llobregat, SpainInstitute of Neuropathology, Bellvitge University Hospital, University of Barcelona, CIBERNED (Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas), 08907 L’Hospitalet de Llobregat, SpainInstitute of Neuropathology, Bellvitge University Hospital, University of Barcelona, CIBERNED (Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas), 08907 L’Hospitalet de Llobregat, SpainInstitute of Neuropathology, Bellvitge University Hospital, University of Barcelona, CIBERNED (Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas), 08907 L’Hospitalet de Llobregat, SpainInflammatory changes are analyzed in the anterior spinal cord and frontal cortex area 8 in typical spinal-predominant ALS cases. Increased numbers of astrocytes and activated microglia are found in the anterior horn of the spinal cord and pyramidal tracts. Significant increased expression of TLR7, CTSS, and CTSC mRNA and a trend to increased expression of IL10RA, TGFB1, and TGFB2 are found in the anterior lumbar spinal cord in ALS cases compared to control cases, whereas C1QTNF7 and TNFRSF1A mRNA expression levels are significantly decreased. IL6 is significantly upregulated and IL1B shows a nonsignificant increased expression in frontal cortex area 8 in ALS cases. IL-6 immunoreactivity is found in scattered monocyte-derived macrophages/microglia and TNF-α in a few cells of unknown origin in ALS cases. Increased expression and abnormal distribution of IL-1β occurred in motor neurons of the lumbar spinal cord in ALS. Strong IL-10 immunoreactivity colocalizes with TDP-43-positive inclusions in motor neurons in ALS cases. The present observations show a complex participation of cytokines and mediators of the inflammatory response in ALS consistent with increased proinflammatory cytokines and sequestration of anti-inflammatory IL-10 in affected neurons.http://dx.doi.org/10.1155/2015/573784 |
| spellingShingle | Sara Berjaoui Mónica Povedano Paula Garcia-Esparcia Margarita Carmona Ester Aso Isidre Ferrer Complex Inflammation mRNA-Related Response in ALS Is Region Dependent Neural Plasticity |
| title | Complex Inflammation mRNA-Related Response in ALS Is Region Dependent |
| title_full | Complex Inflammation mRNA-Related Response in ALS Is Region Dependent |
| title_fullStr | Complex Inflammation mRNA-Related Response in ALS Is Region Dependent |
| title_full_unstemmed | Complex Inflammation mRNA-Related Response in ALS Is Region Dependent |
| title_short | Complex Inflammation mRNA-Related Response in ALS Is Region Dependent |
| title_sort | complex inflammation mrna related response in als is region dependent |
| url | http://dx.doi.org/10.1155/2015/573784 |
| work_keys_str_mv | AT saraberjaoui complexinflammationmrnarelatedresponseinalsisregiondependent AT monicapovedano complexinflammationmrnarelatedresponseinalsisregiondependent AT paulagarciaesparcia complexinflammationmrnarelatedresponseinalsisregiondependent AT margaritacarmona complexinflammationmrnarelatedresponseinalsisregiondependent AT esteraso complexinflammationmrnarelatedresponseinalsisregiondependent AT isidreferrer complexinflammationmrnarelatedresponseinalsisregiondependent |