Physicochemical, antioxidant, and antimicrobial study in Nepalese honey

Five honey samples, mostly from western Nepal, were analyzed for physicochemical properties, TPC, TFC, antioxidant, and antimicrobial assays. The results were found to be within the acceptable range, while some samples had higher moisture, HMF, and total ash levels. The TPC was in the range of 8.42...

Full description

Saved in:
Bibliographic Details
Main Authors: Amrita Adhikari, Nabin Kumar Thapa, Anita Gurung, Niranjan Parajuli
Format: Article
Language:English
Published: Department of Physics, Mahendra Morang Adarsh Multiple Campus, Tribhuvan University 2025-01-01
Series:Bibechana
Subjects:
Online Access:https://www.nepjol.info/index.php/BIBECHANA/article/view/74118
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832584501295841280
author Amrita Adhikari
Nabin Kumar Thapa
Anita Gurung
Niranjan Parajuli
author_facet Amrita Adhikari
Nabin Kumar Thapa
Anita Gurung
Niranjan Parajuli
author_sort Amrita Adhikari
collection DOAJ
description Five honey samples, mostly from western Nepal, were analyzed for physicochemical properties, TPC, TFC, antioxidant, and antimicrobial assays. The results were found to be within the acceptable range, while some samples had higher moisture, HMF, and total ash levels. The TPC was in the range of 8.420 to 9.920 mg GAE and the TFC was between 0.039 to 0.103 mg QE. The IC50 values were calculated of which Forest honey was found to have the lowest IC50 value i.e. 7.735 ± 0.008 mg/mL. Additionally, samples also showed a large zone of inhibition against four pathogens. Besides, the correlation between physicochemical parameters, TFC, TPC, and antioxidant activity was established by using Pearson’s correlation and PCA. It displayed that the variables IC50 , reducing sugar, HMF, and TFC in samples were positively correlated with one another and inversely with total ash (%), acidity as formic acid (%), and moisture (%).
format Article
id doaj-art-17735879fd1e4879998ea876c0f704c7
institution Kabale University
issn 2091-0762
2382-5340
language English
publishDate 2025-01-01
publisher Department of Physics, Mahendra Morang Adarsh Multiple Campus, Tribhuvan University
record_format Article
series Bibechana
spelling doaj-art-17735879fd1e4879998ea876c0f704c72025-01-27T13:14:21ZengDepartment of Physics, Mahendra Morang Adarsh Multiple Campus, Tribhuvan UniversityBibechana2091-07622382-53402025-01-01221Physicochemical, antioxidant, and antimicrobial study in Nepalese honeyAmrita Adhikari0Nabin Kumar Thapa1Anita Gurung2Niranjan Parajuli3Central Department of Chemistry, Tribhuvan University, Kathmandu, NepalCentral Department of Chemistry, Tribhuvan University, Kathmandu, NepalCentral Department of Chemistry, Tribhuvan University, Kathmandu, NepalCentral Department of Chemistry, Tribhuvan University, Kathmandu, Nepal Five honey samples, mostly from western Nepal, were analyzed for physicochemical properties, TPC, TFC, antioxidant, and antimicrobial assays. The results were found to be within the acceptable range, while some samples had higher moisture, HMF, and total ash levels. The TPC was in the range of 8.420 to 9.920 mg GAE and the TFC was between 0.039 to 0.103 mg QE. The IC50 values were calculated of which Forest honey was found to have the lowest IC50 value i.e. 7.735 ± 0.008 mg/mL. Additionally, samples also showed a large zone of inhibition against four pathogens. Besides, the correlation between physicochemical parameters, TFC, TPC, and antioxidant activity was established by using Pearson’s correlation and PCA. It displayed that the variables IC50 , reducing sugar, HMF, and TFC in samples were positively correlated with one another and inversely with total ash (%), acidity as formic acid (%), and moisture (%). https://www.nepjol.info/index.php/BIBECHANA/article/view/74118Physicochemical parametersAntioxidantAntibacterial
spellingShingle Amrita Adhikari
Nabin Kumar Thapa
Anita Gurung
Niranjan Parajuli
Physicochemical, antioxidant, and antimicrobial study in Nepalese honey
Bibechana
Physicochemical parameters
Antioxidant
Antibacterial
title Physicochemical, antioxidant, and antimicrobial study in Nepalese honey
title_full Physicochemical, antioxidant, and antimicrobial study in Nepalese honey
title_fullStr Physicochemical, antioxidant, and antimicrobial study in Nepalese honey
title_full_unstemmed Physicochemical, antioxidant, and antimicrobial study in Nepalese honey
title_short Physicochemical, antioxidant, and antimicrobial study in Nepalese honey
title_sort physicochemical antioxidant and antimicrobial study in nepalese honey
topic Physicochemical parameters
Antioxidant
Antibacterial
url https://www.nepjol.info/index.php/BIBECHANA/article/view/74118
work_keys_str_mv AT amritaadhikari physicochemicalantioxidantandantimicrobialstudyinnepalesehoney
AT nabinkumarthapa physicochemicalantioxidantandantimicrobialstudyinnepalesehoney
AT anitagurung physicochemicalantioxidantandantimicrobialstudyinnepalesehoney
AT niranjanparajuli physicochemicalantioxidantandantimicrobialstudyinnepalesehoney