Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study

BACKGROUND: The prevalence of obesity, or an excessive fat accumulation, is keep increasing. In obesity, inflammation can be induced by leaky gut due to the intestinal tight junction barrier dysfunction. Zonula occludens-1 (ZO-1) plays a role in developing intestinal tight junction barrier dysfuncti...

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Main Authors: Armanto Makmun, Agussalim Bukhari, Nurpudji Astuti Taslim, Aminuddin Aminuddin, Ferry Sandra
Format: Article
Language:English
Published: Secretariat of The Indonesian Biomedical Journal 2024-10-01
Series:Indonesian Biomedical Journal
Online Access:https://inabj.org/index.php/ibj/article/view/3250
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author Armanto Makmun
Agussalim Bukhari
Nurpudji Astuti Taslim
Aminuddin Aminuddin
Ferry Sandra
author_facet Armanto Makmun
Agussalim Bukhari
Nurpudji Astuti Taslim
Aminuddin Aminuddin
Ferry Sandra
author_sort Armanto Makmun
collection DOAJ
description BACKGROUND: The prevalence of obesity, or an excessive fat accumulation, is keep increasing. In obesity, inflammation can be induced by leaky gut due to the intestinal tight junction barrier dysfunction. Zonula occludens-1 (ZO-1) plays a role in developing intestinal tight junction barrier dysfunction and gut microbiota imbalance, thus promote the translocation of bacterial endotoxin characterized by lipopolysaccharide (LPS) into circulation. Black rice extract (BRE) has been known to have anti-inflammatory property. This study was conducted to investigate the effect of BRE on body weight (BW), waist circumference (WC), body mass index (BMI), ZO-1 and LPS of obese patients. METHODS: Twenty-three male subjects were divided into non-obese group (NOG), obese group (COG) and BRE-obese group (BOG). Subjects in BOG received a daily dose of 5.6 g/day BRE for 4 weeks. BW, WC and BMI, serum ZO-1 and LPS were measured before and after treatment. RESULTS: BRE was prepared successfully and free from microbial contamination. Treatment of BRE for 4 weeks reduce BW (95.40±5.78 vs. 94.59±6.00 kg, p=0.043), WC (109.25±3.55 vs. 107.50±3.46 cm, p=0.000) BMI (32.65±1.86 vs. 32.18±1.80, p=0.000) and LPS (222.27±38.63 vs. 131.63±9.70 ng/mL, p=0.020) of obese subjects. The pre-post ZO-1 levels in all groups were not significantly different (p>0.05). CONCLUSION: Treatment of 5.6 gr BRE daily for four weeks can reduce BW, WC, BMI and serum LPS, but not serum ZO-1 in obese patients. Therefore, BRE may reduce inflammation in obesity. KEYWORDS: black rice, obesity, BW, WC, BMI, LPS, ZO-1
format Article
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issn 2085-3297
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language English
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publisher Secretariat of The Indonesian Biomedical Journal
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spelling doaj-art-176ebffbc24a446a845e4c4ba022d5e32025-08-20T01:47:25ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792024-10-011654596310.18585/inabj.v16i5.3250599Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary StudyArmanto Makmun0Agussalim Bukhari1Nurpudji Astuti Taslim2Aminuddin Aminuddin3Ferry Sandra4Faculty of Medicine, Moslem Universitas Muslim Indonesia, Jl. Urip Sumoharjo Km.5, Makassar 90231Department of Nutrition, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km.10, Makassar 90245Department of Nutrition, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km.10, Makassar 90245Department of Nutrition, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km.10, Makassar 90245Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, Jakarta 11440BACKGROUND: The prevalence of obesity, or an excessive fat accumulation, is keep increasing. In obesity, inflammation can be induced by leaky gut due to the intestinal tight junction barrier dysfunction. Zonula occludens-1 (ZO-1) plays a role in developing intestinal tight junction barrier dysfunction and gut microbiota imbalance, thus promote the translocation of bacterial endotoxin characterized by lipopolysaccharide (LPS) into circulation. Black rice extract (BRE) has been known to have anti-inflammatory property. This study was conducted to investigate the effect of BRE on body weight (BW), waist circumference (WC), body mass index (BMI), ZO-1 and LPS of obese patients. METHODS: Twenty-three male subjects were divided into non-obese group (NOG), obese group (COG) and BRE-obese group (BOG). Subjects in BOG received a daily dose of 5.6 g/day BRE for 4 weeks. BW, WC and BMI, serum ZO-1 and LPS were measured before and after treatment. RESULTS: BRE was prepared successfully and free from microbial contamination. Treatment of BRE for 4 weeks reduce BW (95.40±5.78 vs. 94.59±6.00 kg, p=0.043), WC (109.25±3.55 vs. 107.50±3.46 cm, p=0.000) BMI (32.65±1.86 vs. 32.18±1.80, p=0.000) and LPS (222.27±38.63 vs. 131.63±9.70 ng/mL, p=0.020) of obese subjects. The pre-post ZO-1 levels in all groups were not significantly different (p>0.05). CONCLUSION: Treatment of 5.6 gr BRE daily for four weeks can reduce BW, WC, BMI and serum LPS, but not serum ZO-1 in obese patients. Therefore, BRE may reduce inflammation in obesity. KEYWORDS: black rice, obesity, BW, WC, BMI, LPS, ZO-1https://inabj.org/index.php/ibj/article/view/3250
spellingShingle Armanto Makmun
Agussalim Bukhari
Nurpudji Astuti Taslim
Aminuddin Aminuddin
Ferry Sandra
Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study
Indonesian Biomedical Journal
title Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study
title_full Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study
title_fullStr Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study
title_full_unstemmed Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study
title_short Black Rice Extract Reduces Body Weight, Waist Circumference, Body Mass Index and Lipopolysaccharide in Obese Subjects: A Preliminary Study
title_sort black rice extract reduces body weight waist circumference body mass index and lipopolysaccharide in obese subjects a preliminary study
url https://inabj.org/index.php/ibj/article/view/3250
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