Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer
The pancreas, previously considered a sterile organ, has recently been shown to harbor its own microbiota that may influence tumor biology and patient outcomes. Despite increasing interest in the impact of the microbiome on cancer, the relationship between pancreatic tissue and oral microbiomes in p...
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MDPI AG
2025-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/1/119 |
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| author | Alper Uguz Can Muftuoglu Ufuk Mert Tufan Gumus Deniz Ece Milad Asadi Ozlem Ulusan Bagci Ayse Caner |
| author_facet | Alper Uguz Can Muftuoglu Ufuk Mert Tufan Gumus Deniz Ece Milad Asadi Ozlem Ulusan Bagci Ayse Caner |
| author_sort | Alper Uguz |
| collection | DOAJ |
| description | The pancreas, previously considered a sterile organ, has recently been shown to harbor its own microbiota that may influence tumor biology and patient outcomes. Despite increasing interest in the impact of the microbiome on cancer, the relationship between pancreatic tissue and oral microbiomes in pancreatic ductal adenocarcinoma (PDAC) remains limited. In this study, the oral and pancreas tissue microbiomes of patients with PDAC were compared to patients with other periampullary cancers (DC/AC) and a healthy control group using 16S rRNA gene sequence analysis. The results showed a significant reduction in microbial diversity in the saliva of cancer patients compared to healthy controls, while the PDAC patients exhibited a distinct microbial profile in their pancreatic tissues, consisting predominantly of Firmicutes, Proteobacteria, and Actinobacter, after filtering the microbiome of the indoor environment. Notably, the presence of oral bacteria such as <i>Anoxybacillus</i>, <i>Clostridium</i>, and <i>Bacillus</i> in pancreatic tissues suggests potential translocation from the oral cavity. This study emphasizes the importance of understanding the role of body fluid and tissue microbiota in pancreatic cancer, proposing that oral dysbiosis may contribute to disease progression. Moreover, the results suggest that the microbiome of the indoor environment in which samples are collected and analyzed is also important in microbiota analysis studies. |
| format | Article |
| id | doaj-art-176b6500b7c9434a9950b8d8a810cfe3 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-176b6500b7c9434a9950b8d8a810cfe32025-01-24T13:42:43ZengMDPI AGMicroorganisms2076-26072025-01-0113111910.3390/microorganisms13010119Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic CancerAlper Uguz0Can Muftuoglu1Ufuk Mert2Tufan Gumus3Deniz Ece4Milad Asadi5Ozlem Ulusan Bagci6Ayse Caner7Department of General Surgery, Faculty of Medicine, Ege University, 35040 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyAtatürk Vocational School of Health Services, Ege University, 35100 Izmir, TurkeyDepartment of General Surgery, Faculty of Medicine, Ege University, 35040 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyThe pancreas, previously considered a sterile organ, has recently been shown to harbor its own microbiota that may influence tumor biology and patient outcomes. Despite increasing interest in the impact of the microbiome on cancer, the relationship between pancreatic tissue and oral microbiomes in pancreatic ductal adenocarcinoma (PDAC) remains limited. In this study, the oral and pancreas tissue microbiomes of patients with PDAC were compared to patients with other periampullary cancers (DC/AC) and a healthy control group using 16S rRNA gene sequence analysis. The results showed a significant reduction in microbial diversity in the saliva of cancer patients compared to healthy controls, while the PDAC patients exhibited a distinct microbial profile in their pancreatic tissues, consisting predominantly of Firmicutes, Proteobacteria, and Actinobacter, after filtering the microbiome of the indoor environment. Notably, the presence of oral bacteria such as <i>Anoxybacillus</i>, <i>Clostridium</i>, and <i>Bacillus</i> in pancreatic tissues suggests potential translocation from the oral cavity. This study emphasizes the importance of understanding the role of body fluid and tissue microbiota in pancreatic cancer, proposing that oral dysbiosis may contribute to disease progression. Moreover, the results suggest that the microbiome of the indoor environment in which samples are collected and analyzed is also important in microbiota analysis studies.https://www.mdpi.com/2076-2607/13/1/119pancreatic ductal adenocarcinomadistal cholangiocarcinomaampullary canceroral microbiomeintrapancreatic microbiomesaline control samples |
| spellingShingle | Alper Uguz Can Muftuoglu Ufuk Mert Tufan Gumus Deniz Ece Milad Asadi Ozlem Ulusan Bagci Ayse Caner Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer Microorganisms pancreatic ductal adenocarcinoma distal cholangiocarcinoma ampullary cancer oral microbiome intrapancreatic microbiome saline control samples |
| title | Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer |
| title_full | Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer |
| title_fullStr | Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer |
| title_full_unstemmed | Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer |
| title_short | Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer |
| title_sort | unveiling microbiota profiles in saliva and pancreatic tissues of patients with pancreatic cancer |
| topic | pancreatic ductal adenocarcinoma distal cholangiocarcinoma ampullary cancer oral microbiome intrapancreatic microbiome saline control samples |
| url | https://www.mdpi.com/2076-2607/13/1/119 |
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