Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer

The pancreas, previously considered a sterile organ, has recently been shown to harbor its own microbiota that may influence tumor biology and patient outcomes. Despite increasing interest in the impact of the microbiome on cancer, the relationship between pancreatic tissue and oral microbiomes in p...

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Main Authors: Alper Uguz, Can Muftuoglu, Ufuk Mert, Tufan Gumus, Deniz Ece, Milad Asadi, Ozlem Ulusan Bagci, Ayse Caner
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/1/119
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author Alper Uguz
Can Muftuoglu
Ufuk Mert
Tufan Gumus
Deniz Ece
Milad Asadi
Ozlem Ulusan Bagci
Ayse Caner
author_facet Alper Uguz
Can Muftuoglu
Ufuk Mert
Tufan Gumus
Deniz Ece
Milad Asadi
Ozlem Ulusan Bagci
Ayse Caner
author_sort Alper Uguz
collection DOAJ
description The pancreas, previously considered a sterile organ, has recently been shown to harbor its own microbiota that may influence tumor biology and patient outcomes. Despite increasing interest in the impact of the microbiome on cancer, the relationship between pancreatic tissue and oral microbiomes in pancreatic ductal adenocarcinoma (PDAC) remains limited. In this study, the oral and pancreas tissue microbiomes of patients with PDAC were compared to patients with other periampullary cancers (DC/AC) and a healthy control group using 16S rRNA gene sequence analysis. The results showed a significant reduction in microbial diversity in the saliva of cancer patients compared to healthy controls, while the PDAC patients exhibited a distinct microbial profile in their pancreatic tissues, consisting predominantly of Firmicutes, Proteobacteria, and Actinobacter, after filtering the microbiome of the indoor environment. Notably, the presence of oral bacteria such as <i>Anoxybacillus</i>, <i>Clostridium</i>, and <i>Bacillus</i> in pancreatic tissues suggests potential translocation from the oral cavity. This study emphasizes the importance of understanding the role of body fluid and tissue microbiota in pancreatic cancer, proposing that oral dysbiosis may contribute to disease progression. Moreover, the results suggest that the microbiome of the indoor environment in which samples are collected and analyzed is also important in microbiota analysis studies.
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institution Kabale University
issn 2076-2607
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publishDate 2025-01-01
publisher MDPI AG
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series Microorganisms
spelling doaj-art-176b6500b7c9434a9950b8d8a810cfe32025-01-24T13:42:43ZengMDPI AGMicroorganisms2076-26072025-01-0113111910.3390/microorganisms13010119Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic CancerAlper Uguz0Can Muftuoglu1Ufuk Mert2Tufan Gumus3Deniz Ece4Milad Asadi5Ozlem Ulusan Bagci6Ayse Caner7Department of General Surgery, Faculty of Medicine, Ege University, 35040 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyAtatürk Vocational School of Health Services, Ege University, 35100 Izmir, TurkeyDepartment of General Surgery, Faculty of Medicine, Ege University, 35040 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyDepartment of Basic Oncology, Institute of Health Sciences, Ege University, 35100 Izmir, TurkeyThe pancreas, previously considered a sterile organ, has recently been shown to harbor its own microbiota that may influence tumor biology and patient outcomes. Despite increasing interest in the impact of the microbiome on cancer, the relationship between pancreatic tissue and oral microbiomes in pancreatic ductal adenocarcinoma (PDAC) remains limited. In this study, the oral and pancreas tissue microbiomes of patients with PDAC were compared to patients with other periampullary cancers (DC/AC) and a healthy control group using 16S rRNA gene sequence analysis. The results showed a significant reduction in microbial diversity in the saliva of cancer patients compared to healthy controls, while the PDAC patients exhibited a distinct microbial profile in their pancreatic tissues, consisting predominantly of Firmicutes, Proteobacteria, and Actinobacter, after filtering the microbiome of the indoor environment. Notably, the presence of oral bacteria such as <i>Anoxybacillus</i>, <i>Clostridium</i>, and <i>Bacillus</i> in pancreatic tissues suggests potential translocation from the oral cavity. This study emphasizes the importance of understanding the role of body fluid and tissue microbiota in pancreatic cancer, proposing that oral dysbiosis may contribute to disease progression. Moreover, the results suggest that the microbiome of the indoor environment in which samples are collected and analyzed is also important in microbiota analysis studies.https://www.mdpi.com/2076-2607/13/1/119pancreatic ductal adenocarcinomadistal cholangiocarcinomaampullary canceroral microbiomeintrapancreatic microbiomesaline control samples
spellingShingle Alper Uguz
Can Muftuoglu
Ufuk Mert
Tufan Gumus
Deniz Ece
Milad Asadi
Ozlem Ulusan Bagci
Ayse Caner
Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer
Microorganisms
pancreatic ductal adenocarcinoma
distal cholangiocarcinoma
ampullary cancer
oral microbiome
intrapancreatic microbiome
saline control samples
title Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer
title_full Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer
title_fullStr Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer
title_full_unstemmed Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer
title_short Unveiling Microbiota Profiles in Saliva and Pancreatic Tissues of Patients with Pancreatic Cancer
title_sort unveiling microbiota profiles in saliva and pancreatic tissues of patients with pancreatic cancer
topic pancreatic ductal adenocarcinoma
distal cholangiocarcinoma
ampullary cancer
oral microbiome
intrapancreatic microbiome
saline control samples
url https://www.mdpi.com/2076-2607/13/1/119
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