Prevalence, Antibiotic Susceptibility, and Molecular Typing of Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) among Domestic Animals in Dharan, Nepal
Staphylococcus aureus, a commensal bacterium found on the skin, skin glands, and mucous membranes of humans and animals. It affects the skin, soft tissues, bloodstream, and lower respiratory tract, causing diseases such as endocarditis, osteomyelitis, and generalized scalded skin disease in animals...
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Biotechnology Society of Nepal
2024-12-01
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| Series: | Nepal Journal of Biotechnology |
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| Online Access: | https://www.nepjb.com/index.php/NJB/article/view/335 |
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| author | Rubina Katuwal Manita Shyaula Bigyan Shrestha Saroj Babu Koirala Hemanta Khanal |
| author_facet | Rubina Katuwal Manita Shyaula Bigyan Shrestha Saroj Babu Koirala Hemanta Khanal |
| author_sort | Rubina Katuwal |
| collection | DOAJ |
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Staphylococcus aureus, a commensal bacterium found on the skin, skin glands, and mucous membranes of humans and animals. It affects the skin, soft tissues, bloodstream, and lower respiratory tract, causing diseases such as endocarditis, osteomyelitis, and generalized scalded skin disease in animals. This study investigates the prevalence and antibiotic susceptibility patterns of S. aureus and Methicillin-resistant S. aureus (MRSA) among domestic animals in Dharan, Nepal. A total of 320 skin swab samples were collected from various domestic animals, revealing a 12.5% prevalence rate of S. aureus. Strikingly, MRSA prevalence was notably high at 60% among the isolated S. aureus strains. Antimicrobial susceptibility testing unveiled widespread resistance to commonly used antibiotics, with notable exceptions such as vancomycin. Molecular analysis using the spa gene as a marker for strain typing detected its presence in a small subset of MRSA isolates, suggesting the possibility of human colonization and further underscoring the interconnectedness of animal and human health. Only 5% of the MRSA isolates carried the spa gene, confirmed by PCR and gel documentation in samples from buffalo skin swabs. The study highlights the importance of interdisciplinary collaboration between veterinary and human medicine sectors to address the growing threat of antimicrobial resistance. Overall, this research contributes valuable insights into the epidemiology of S. aureus infections in livestock and advocates for stringent hygiene practices, prudent antibiotic usage, and routine veterinary monitoring to mitigate the spread of MRSA and protect public health interests.
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| format | Article |
| id | doaj-art-17572435733b4f11af54ff726ec421c7 |
| institution | Kabale University |
| issn | 2091-1130 2467-9313 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Biotechnology Society of Nepal |
| record_format | Article |
| series | Nepal Journal of Biotechnology |
| spelling | doaj-art-17572435733b4f11af54ff726ec421c72025-01-07T14:47:04ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132024-12-0112210.54796/njb.v12i2.335Prevalence, Antibiotic Susceptibility, and Molecular Typing of Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) among Domestic Animals in Dharan, NepalRubina Katuwal0Manita Shyaula1Bigyan Shrestha2Saroj Babu Koirala3Hemanta Khanal4Department of Microbiology, Central Campus of Technology, Tribhuvan University, Dharan, NepalBiological Resource Unit, Faculty of Science, Nepal Academy of Science and Technology, Lalitpur, Nepal Department of Microbiology, Central Campus of Technology, Tribhuvan University, Dharan, NepalBioenergy Lab, Faculty of Technology, Nepal Academy of Science and Technology, Lalitpur, Nepal Department of Microbiology, Central Campus of Technology, Tribhuvan University, Dharan, Nepal Staphylococcus aureus, a commensal bacterium found on the skin, skin glands, and mucous membranes of humans and animals. It affects the skin, soft tissues, bloodstream, and lower respiratory tract, causing diseases such as endocarditis, osteomyelitis, and generalized scalded skin disease in animals. This study investigates the prevalence and antibiotic susceptibility patterns of S. aureus and Methicillin-resistant S. aureus (MRSA) among domestic animals in Dharan, Nepal. A total of 320 skin swab samples were collected from various domestic animals, revealing a 12.5% prevalence rate of S. aureus. Strikingly, MRSA prevalence was notably high at 60% among the isolated S. aureus strains. Antimicrobial susceptibility testing unveiled widespread resistance to commonly used antibiotics, with notable exceptions such as vancomycin. Molecular analysis using the spa gene as a marker for strain typing detected its presence in a small subset of MRSA isolates, suggesting the possibility of human colonization and further underscoring the interconnectedness of animal and human health. Only 5% of the MRSA isolates carried the spa gene, confirmed by PCR and gel documentation in samples from buffalo skin swabs. The study highlights the importance of interdisciplinary collaboration between veterinary and human medicine sectors to address the growing threat of antimicrobial resistance. Overall, this research contributes valuable insights into the epidemiology of S. aureus infections in livestock and advocates for stringent hygiene practices, prudent antibiotic usage, and routine veterinary monitoring to mitigate the spread of MRSA and protect public health interests. https://www.nepjb.com/index.php/NJB/article/view/335Staphylococcus aureusdomestic animalsantibiotic susceptibilitySpa gene |
| spellingShingle | Rubina Katuwal Manita Shyaula Bigyan Shrestha Saroj Babu Koirala Hemanta Khanal Prevalence, Antibiotic Susceptibility, and Molecular Typing of Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) among Domestic Animals in Dharan, Nepal Nepal Journal of Biotechnology Staphylococcus aureus domestic animals antibiotic susceptibility Spa gene |
| title | Prevalence, Antibiotic Susceptibility, and Molecular Typing of Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) among Domestic Animals in Dharan, Nepal |
| title_full | Prevalence, Antibiotic Susceptibility, and Molecular Typing of Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) among Domestic Animals in Dharan, Nepal |
| title_fullStr | Prevalence, Antibiotic Susceptibility, and Molecular Typing of Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) among Domestic Animals in Dharan, Nepal |
| title_full_unstemmed | Prevalence, Antibiotic Susceptibility, and Molecular Typing of Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) among Domestic Animals in Dharan, Nepal |
| title_short | Prevalence, Antibiotic Susceptibility, and Molecular Typing of Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) among Domestic Animals in Dharan, Nepal |
| title_sort | prevalence antibiotic susceptibility and molecular typing of staphylococcus aureus and methicillin resistant s aureus mrsa among domestic animals in dharan nepal |
| topic | Staphylococcus aureus domestic animals antibiotic susceptibility Spa gene |
| url | https://www.nepjb.com/index.php/NJB/article/view/335 |
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