Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification.
To respond to the urgent need for COVID-19 testing, countries perform nucleic acid amplification tests (NAAT) for the detection of SARS-CoV-2 in centralized laboratories. Real-time RT-PCR (Reverse transcription-Polymerase Chain Reaction), used to amplify and detect the viral RNA., is considered, as...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0243712&type=printable |
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| author | Pierre Garneret Etienne Coz Elian Martin Jean-Claude Manuguerra Elodie Brient-Litzler Vincent Enouf Daniel Felipe González Obando Jean-Christophe Olivo-Marin Fabrice Monti Sylvie van der Werf Jessica Vanhomwegen Patrick Tabeling |
| author_facet | Pierre Garneret Etienne Coz Elian Martin Jean-Claude Manuguerra Elodie Brient-Litzler Vincent Enouf Daniel Felipe González Obando Jean-Christophe Olivo-Marin Fabrice Monti Sylvie van der Werf Jessica Vanhomwegen Patrick Tabeling |
| author_sort | Pierre Garneret |
| collection | DOAJ |
| description | To respond to the urgent need for COVID-19 testing, countries perform nucleic acid amplification tests (NAAT) for the detection of SARS-CoV-2 in centralized laboratories. Real-time RT-PCR (Reverse transcription-Polymerase Chain Reaction), used to amplify and detect the viral RNA., is considered, as the current gold standard for diagnostics. It is an efficient process, but the complex engineering required for automated RNA extraction and temperature cycling makes it incompatible for use in point of care settings [1]. In the present work, by harnessing progress made in the past two decades in isothermal amplification and paper microfluidics, we created a portable test, in which SARS-CoV-2 RNA is extracted, amplified isothermally by RT-LAMP (Loop-mediated Isothermal Amplification), and detected using intercalating dyes or fluorescent probes. Depending on the viral load in the tested samples, the detection takes between twenty minutes and one hour. Using a set of 16 pools of naso-pharyngal swab eluates, we estimated a limit of detection comparable to real-time RT-PCR (i.e. 1 genome copies per microliter of clinical sample) and no cross-reaction with eight major respiratory viruses currently circulating in Europe. We designed and fabricated an easy-to-use portable device called "COVIDISC" to carry out the test at the point of care. The low cost of the materials along with the absence of complex equipment will expedite the widespread dissemination of this device. What is proposed here is a new efficient tool to help managing the pandemics. |
| format | Article |
| id | doaj-art-1756a12232aa46c398a66d5bca4916e8 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-1756a12232aa46c398a66d5bca4916e82025-08-20T02:55:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01161e024371210.1371/journal.pone.0243712Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification.Pierre GarneretEtienne CozElian MartinJean-Claude ManuguerraElodie Brient-LitzlerVincent EnoufDaniel Felipe González ObandoJean-Christophe Olivo-MarinFabrice MontiSylvie van der WerfJessica VanhomwegenPatrick TabelingTo respond to the urgent need for COVID-19 testing, countries perform nucleic acid amplification tests (NAAT) for the detection of SARS-CoV-2 in centralized laboratories. Real-time RT-PCR (Reverse transcription-Polymerase Chain Reaction), used to amplify and detect the viral RNA., is considered, as the current gold standard for diagnostics. It is an efficient process, but the complex engineering required for automated RNA extraction and temperature cycling makes it incompatible for use in point of care settings [1]. In the present work, by harnessing progress made in the past two decades in isothermal amplification and paper microfluidics, we created a portable test, in which SARS-CoV-2 RNA is extracted, amplified isothermally by RT-LAMP (Loop-mediated Isothermal Amplification), and detected using intercalating dyes or fluorescent probes. Depending on the viral load in the tested samples, the detection takes between twenty minutes and one hour. Using a set of 16 pools of naso-pharyngal swab eluates, we estimated a limit of detection comparable to real-time RT-PCR (i.e. 1 genome copies per microliter of clinical sample) and no cross-reaction with eight major respiratory viruses currently circulating in Europe. We designed and fabricated an easy-to-use portable device called "COVIDISC" to carry out the test at the point of care. The low cost of the materials along with the absence of complex equipment will expedite the widespread dissemination of this device. What is proposed here is a new efficient tool to help managing the pandemics.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0243712&type=printable |
| spellingShingle | Pierre Garneret Etienne Coz Elian Martin Jean-Claude Manuguerra Elodie Brient-Litzler Vincent Enouf Daniel Felipe González Obando Jean-Christophe Olivo-Marin Fabrice Monti Sylvie van der Werf Jessica Vanhomwegen Patrick Tabeling Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification. PLoS ONE |
| title | Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification. |
| title_full | Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification. |
| title_fullStr | Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification. |
| title_full_unstemmed | Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification. |
| title_short | Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification. |
| title_sort | performing point of care molecular testing for sars cov 2 with rna extraction and isothermal amplification |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0243712&type=printable |
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