Synergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing: a systematic review and meta-analysis of preclinical studies
Abstract Background Burn injuries cause severe physical and psychological challenges. Current treatments have limitations, such as incomplete skin regeneration and prolonged healing times, necessitating innovative solutions. Tissue engineering integrates mesenchymal stem cells (MSCs) and scaffolds i...
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| Language: | English |
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BMC
2025-07-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-025-06712-y |
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| author | Amir Hossein Aghayan Davood Mohammadi Amir Atashi Zahra Jamalpoor |
| author_facet | Amir Hossein Aghayan Davood Mohammadi Amir Atashi Zahra Jamalpoor |
| author_sort | Amir Hossein Aghayan |
| collection | DOAJ |
| description | Abstract Background Burn injuries cause severe physical and psychological challenges. Current treatments have limitations, such as incomplete skin regeneration and prolonged healing times, necessitating innovative solutions. Tissue engineering integrates mesenchymal stem cells (MSCs) and scaffolds in preclinical studies to enhance healing, reduce scarring, and regenerate skin. This study examines their combined effects to optimize burn wound therapies. Methods This systematic review and meta-analysis was conducted per PRISMA guidelines, with the protocol registered in PROSPERO. Databases (PubMed, Embase, Scopus, Web of Science, ProQuest) were searched for published studies.The SYRCLE tool assessed risk of bias. Standardized mean difference (SMD) evaluated the therapeutic effect of MSC-scaffold combinations. Statistical analysis employed a random-effects model. Sensitivity analysis, publication bias assessment, and subgroup analysis addressed heterogeneity. Results Our meta-analysis reveals that animals treated with MSC-scaffold combinations exhibit enhanced wound closure compared to untreated controls across short-term (SMD = 3.97), mid-term (SMD = 3.47), and long-term (SMD = 3.03) timeframes. Additionally, MSC-scaffold combinations significantly improve angiogenesis (SMD = 6.24), collagen deposition (SMD = 4.97), and growth factor expression (SMD = 6.68), while effectively modulating inflammatory cytokine levels (SMD = -4.88). Conclusion Our research demonstrates that MSC-scaffold synergy in preclinical burn wound healing significantly improves wound closure, collagen deposition, angiogenesis, and growth factor expression, while effectively modulating inflammatory responses. Despite its potential as an adjunctive therapy, substantial study heterogeneity impedes clinical translation, necessitating standardized protocols to refine and validate this approach for efficacious clinical application. Graphical Abstract |
| format | Article |
| id | doaj-art-174ec4178cd0424394eed30c436dc4fa |
| institution | Kabale University |
| issn | 1479-5876 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-174ec4178cd0424394eed30c436dc4fa2025-08-20T03:45:31ZengBMCJournal of Translational Medicine1479-58762025-07-0123112910.1186/s12967-025-06712-ySynergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing: a systematic review and meta-analysis of preclinical studiesAmir Hossein Aghayan0Davood Mohammadi1Amir Atashi2Zahra Jamalpoor3Trauma and Surgery Research Center, Aja University of Medical SciencesTrauma and Surgery Research Center, Aja University of Medical SciencesDepartment of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical SciencesTrauma and Surgery Research Center, Aja University of Medical SciencesAbstract Background Burn injuries cause severe physical and psychological challenges. Current treatments have limitations, such as incomplete skin regeneration and prolonged healing times, necessitating innovative solutions. Tissue engineering integrates mesenchymal stem cells (MSCs) and scaffolds in preclinical studies to enhance healing, reduce scarring, and regenerate skin. This study examines their combined effects to optimize burn wound therapies. Methods This systematic review and meta-analysis was conducted per PRISMA guidelines, with the protocol registered in PROSPERO. Databases (PubMed, Embase, Scopus, Web of Science, ProQuest) were searched for published studies.The SYRCLE tool assessed risk of bias. Standardized mean difference (SMD) evaluated the therapeutic effect of MSC-scaffold combinations. Statistical analysis employed a random-effects model. Sensitivity analysis, publication bias assessment, and subgroup analysis addressed heterogeneity. Results Our meta-analysis reveals that animals treated with MSC-scaffold combinations exhibit enhanced wound closure compared to untreated controls across short-term (SMD = 3.97), mid-term (SMD = 3.47), and long-term (SMD = 3.03) timeframes. Additionally, MSC-scaffold combinations significantly improve angiogenesis (SMD = 6.24), collagen deposition (SMD = 4.97), and growth factor expression (SMD = 6.68), while effectively modulating inflammatory cytokine levels (SMD = -4.88). Conclusion Our research demonstrates that MSC-scaffold synergy in preclinical burn wound healing significantly improves wound closure, collagen deposition, angiogenesis, and growth factor expression, while effectively modulating inflammatory responses. Despite its potential as an adjunctive therapy, substantial study heterogeneity impedes clinical translation, necessitating standardized protocols to refine and validate this approach for efficacious clinical application. Graphical Abstracthttps://doi.org/10.1186/s12967-025-06712-yMesenchymal stem cellsExosomeScaffoldMatrixHydrogelBurn |
| spellingShingle | Amir Hossein Aghayan Davood Mohammadi Amir Atashi Zahra Jamalpoor Synergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing: a systematic review and meta-analysis of preclinical studies Journal of Translational Medicine Mesenchymal stem cells Exosome Scaffold Matrix Hydrogel Burn |
| title | Synergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing: a systematic review and meta-analysis of preclinical studies |
| title_full | Synergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing: a systematic review and meta-analysis of preclinical studies |
| title_fullStr | Synergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing: a systematic review and meta-analysis of preclinical studies |
| title_full_unstemmed | Synergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing: a systematic review and meta-analysis of preclinical studies |
| title_short | Synergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing: a systematic review and meta-analysis of preclinical studies |
| title_sort | synergistic effects of mesenchymal stem cells and their secretomes with scaffolds in burn wound healing a systematic review and meta analysis of preclinical studies |
| topic | Mesenchymal stem cells Exosome Scaffold Matrix Hydrogel Burn |
| url | https://doi.org/10.1186/s12967-025-06712-y |
| work_keys_str_mv | AT amirhosseinaghayan synergisticeffectsofmesenchymalstemcellsandtheirsecretomeswithscaffoldsinburnwoundhealingasystematicreviewandmetaanalysisofpreclinicalstudies AT davoodmohammadi synergisticeffectsofmesenchymalstemcellsandtheirsecretomeswithscaffoldsinburnwoundhealingasystematicreviewandmetaanalysisofpreclinicalstudies AT amiratashi synergisticeffectsofmesenchymalstemcellsandtheirsecretomeswithscaffoldsinburnwoundhealingasystematicreviewandmetaanalysisofpreclinicalstudies AT zahrajamalpoor synergisticeffectsofmesenchymalstemcellsandtheirsecretomeswithscaffoldsinburnwoundhealingasystematicreviewandmetaanalysisofpreclinicalstudies |