Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
We isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution tr...
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2014-01-01
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Series: | The Scientific World Journal |
Online Access: | http://dx.doi.org/10.1155/2014/874764 |
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author | Pei-Ling Yong Kok-Gan Chan |
author_facet | Pei-Ling Yong Kok-Gan Chan |
author_sort | Pei-Ling Yong |
collection | DOAJ |
description | We isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) which confirmed the production of multiple AHLs, namely, N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL) and N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL). These findings will open the perspective to study the function of these AHLs in plant-microbe interactions. |
format | Article |
id | doaj-art-174af598b7844d558231bf76eee8cee3 |
institution | Kabale University |
issn | 2356-6140 1537-744X |
language | English |
publishDate | 2014-01-01 |
publisher | Wiley |
record_format | Article |
series | The Scientific World Journal |
spelling | doaj-art-174af598b7844d558231bf76eee8cee32025-02-03T01:06:45ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/874764874764Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable WaterPei-Ling Yong0Kok-Gan Chan1Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaWe isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) which confirmed the production of multiple AHLs, namely, N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL) and N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL). These findings will open the perspective to study the function of these AHLs in plant-microbe interactions.http://dx.doi.org/10.1155/2014/874764 |
spellingShingle | Pei-Ling Yong Kok-Gan Chan Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water The Scientific World Journal |
title | Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water |
title_full | Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water |
title_fullStr | Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water |
title_full_unstemmed | Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water |
title_short | Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water |
title_sort | quorum sensing activity of mesorhizobium sp f7 isolated from potable water |
url | http://dx.doi.org/10.1155/2014/874764 |
work_keys_str_mv | AT peilingyong quorumsensingactivityofmesorhizobiumspf7isolatedfrompotablewater AT kokganchan quorumsensingactivityofmesorhizobiumspf7isolatedfrompotablewater |