Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water

We isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution tr...

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Main Authors: Pei-Ling Yong, Kok-Gan Chan
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2014/874764
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author Pei-Ling Yong
Kok-Gan Chan
author_facet Pei-Ling Yong
Kok-Gan Chan
author_sort Pei-Ling Yong
collection DOAJ
description We isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) which confirmed the production of multiple AHLs, namely, N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL) and N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL). These findings will open the perspective to study the function of these AHLs in plant-microbe interactions.
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institution Kabale University
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publishDate 2014-01-01
publisher Wiley
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series The Scientific World Journal
spelling doaj-art-174af598b7844d558231bf76eee8cee32025-02-03T01:06:45ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/874764874764Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable WaterPei-Ling Yong0Kok-Gan Chan1Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaWe isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) which confirmed the production of multiple AHLs, namely, N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL) and N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL). These findings will open the perspective to study the function of these AHLs in plant-microbe interactions.http://dx.doi.org/10.1155/2014/874764
spellingShingle Pei-Ling Yong
Kok-Gan Chan
Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
The Scientific World Journal
title Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_full Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_fullStr Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_full_unstemmed Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_short Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_sort quorum sensing activity of mesorhizobium sp f7 isolated from potable water
url http://dx.doi.org/10.1155/2014/874764
work_keys_str_mv AT peilingyong quorumsensingactivityofmesorhizobiumspf7isolatedfrompotablewater
AT kokganchan quorumsensingactivityofmesorhizobiumspf7isolatedfrompotablewater