Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells
Zeaxanthin at nonlethal dosages (3–10 μM) significantly inhibited the cell migration of cultured uveal melanoma cells (C918 cell line) as determined by wound healing assay and Boyden chamber assay. Matrigel invasion assay showed that cell invasion of uveal melanoma cells could be significantly inhib...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2016/8734309 |
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| author | Ming-Chao Bi Nicole Hose Cai-Lian Xu Chen Zhang Jodi Sassoon E. Song |
| author_facet | Ming-Chao Bi Nicole Hose Cai-Lian Xu Chen Zhang Jodi Sassoon E. Song |
| author_sort | Ming-Chao Bi |
| collection | DOAJ |
| description | Zeaxanthin at nonlethal dosages (3–10 μM) significantly inhibited the cell migration of cultured uveal melanoma cells (C918 cell line) as determined by wound healing assay and Boyden chamber assay. Matrigel invasion assay showed that cell invasion of uveal melanoma cells could be significantly inhibited by zeaxanthin. Secretion of MMP-2 by melanoma cells was significantly inhibited by zeaxanthin in a dose-dependent manner as measured by ELISA kit. Zeaxanthin also significantly inhibited the NF-κB levels in nuclear extracts of the UM cells, which is the upstream of the MMP-2 secretion. These results suggest that zeaxanthin might be a potentially therapeutic approach in the prevention of metastasis in uveal melanoma. |
| format | Article |
| id | doaj-art-1747818bce2f4274be7583b552fc5441 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-1747818bce2f4274be7583b552fc54412025-02-03T05:58:51ZengWileyJournal of Ophthalmology2090-004X2090-00582016-01-01201610.1155/2016/87343098734309Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma CellsMing-Chao Bi0Nicole Hose1Cai-Lian Xu2Chen Zhang3Jodi Sassoon4E. Song5Department of Ophthalmology, The First Hospital of Jilin University, Changchun 130021, ChinaFordham University, New York, NY 10023, USADepartment of Ophthalmology, The First Hospital of Jilin University, Changchun 130021, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaTissue Culture Center, The New York Eye and Ear Infirmary of Mount Sinai, New York, NY 10003, USADepartment of Ophthalmology, The First Hospital of Jilin University, Changchun 130021, ChinaZeaxanthin at nonlethal dosages (3–10 μM) significantly inhibited the cell migration of cultured uveal melanoma cells (C918 cell line) as determined by wound healing assay and Boyden chamber assay. Matrigel invasion assay showed that cell invasion of uveal melanoma cells could be significantly inhibited by zeaxanthin. Secretion of MMP-2 by melanoma cells was significantly inhibited by zeaxanthin in a dose-dependent manner as measured by ELISA kit. Zeaxanthin also significantly inhibited the NF-κB levels in nuclear extracts of the UM cells, which is the upstream of the MMP-2 secretion. These results suggest that zeaxanthin might be a potentially therapeutic approach in the prevention of metastasis in uveal melanoma.http://dx.doi.org/10.1155/2016/8734309 |
| spellingShingle | Ming-Chao Bi Nicole Hose Cai-Lian Xu Chen Zhang Jodi Sassoon E. Song Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells Journal of Ophthalmology |
| title | Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells |
| title_full | Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells |
| title_fullStr | Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells |
| title_full_unstemmed | Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells |
| title_short | Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells |
| title_sort | nonlethal levels of zeaxanthin inhibit cell migration invasion and secretion of mmp 2 via nf κb pathway in cultured human uveal melanoma cells |
| url | http://dx.doi.org/10.1155/2016/8734309 |
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