The association between serum klotho protein and stroke: a cross-sectional study from NHANES 2007–2016

ObjectiveSerum klotho protein is a protein with anti-aging effects. Since the relationship between serum klotho and Stroke remains rather ambiguous, this research probed into the potential correlation between serum klotho concentration and Stroke.MethodsThis study employed a cross-sectional design a...

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Main Authors: Hongjia Xu, Yiming Ding, Ye Zhang, Jianwen Li, Shiyue Zhou, Dewei Wang, Xinyue Xing, Xiaoyu Ma, Cunfu Wang, Shunliang Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Neurology
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Online Access:https://www.frontiersin.org/articles/10.3389/fneur.2025.1573027/full
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author Hongjia Xu
Yiming Ding
Yiming Ding
Ye Zhang
Jianwen Li
Shiyue Zhou
Dewei Wang
Xinyue Xing
Xiaoyu Ma
Cunfu Wang
Shunliang Xu
Shunliang Xu
author_facet Hongjia Xu
Yiming Ding
Yiming Ding
Ye Zhang
Jianwen Li
Shiyue Zhou
Dewei Wang
Xinyue Xing
Xiaoyu Ma
Cunfu Wang
Shunliang Xu
Shunliang Xu
author_sort Hongjia Xu
collection DOAJ
description ObjectiveSerum klotho protein is a protein with anti-aging effects. Since the relationship between serum klotho and Stroke remains rather ambiguous, this research probed into the potential correlation between serum klotho concentration and Stroke.MethodsThis study employed a cross-sectional design and incorporated population data from the NHANES from 2007 to 2016. Weighted univariate and multivariate logistic regression models were utilized to inspect the relationship between klotho and Stroke. Stratified analyses and interaction tests were carried out to explore the latent correlation between klotho and Stroke. Finally, a fitted smooth curve was adopted to depict the non-linear relationship.ResultsIn this study, after excluding all the missing data, a total of 12,414 participants were encompassed, including 450 Stroke individuals. After adjusting for all covariates, higher klotho was associated with a lower prevalence of Stroke. According to the subgroup analyses and interaction tests, age, gender, race, BMI, hypertension, diabetes mellitus, family members, drinker and smoker were not significantly correlated with the influence of klotho and Stroke. After adjusting for all covariates, higher klotho was associated with a lower prevalence of stroke [OR: 0.68, 95% CI: 0.47–0.99].ConclusionThis study disclosed the negative correlation between serum klotho protein levels and the prevalence of Stroke. Further prospective studies are requisite to investigate the impact of serum klotho protein levels on Stroke and determine the causal relationship.
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publisher Frontiers Media S.A.
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spelling doaj-art-17473072c4974697a4fa464d5df46f4d2025-08-20T02:30:59ZengFrontiers Media S.A.Frontiers in Neurology1664-22952025-05-011610.3389/fneur.2025.15730271573027The association between serum klotho protein and stroke: a cross-sectional study from NHANES 2007–2016Hongjia Xu0Yiming Ding1Yiming Ding2Ye Zhang3Jianwen Li4Shiyue Zhou5Dewei Wang6Xinyue Xing7Xiaoyu Ma8Cunfu Wang9Shunliang Xu10Shunliang Xu11Department of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Pediatric Surgery, Qilu Hospital of Shandong University, Jinan, ChinaCheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Human Genetics, School of Medicine, Emory University, Atlanta, GA, United StatesObjectiveSerum klotho protein is a protein with anti-aging effects. Since the relationship between serum klotho and Stroke remains rather ambiguous, this research probed into the potential correlation between serum klotho concentration and Stroke.MethodsThis study employed a cross-sectional design and incorporated population data from the NHANES from 2007 to 2016. Weighted univariate and multivariate logistic regression models were utilized to inspect the relationship between klotho and Stroke. Stratified analyses and interaction tests were carried out to explore the latent correlation between klotho and Stroke. Finally, a fitted smooth curve was adopted to depict the non-linear relationship.ResultsIn this study, after excluding all the missing data, a total of 12,414 participants were encompassed, including 450 Stroke individuals. After adjusting for all covariates, higher klotho was associated with a lower prevalence of Stroke. According to the subgroup analyses and interaction tests, age, gender, race, BMI, hypertension, diabetes mellitus, family members, drinker and smoker were not significantly correlated with the influence of klotho and Stroke. After adjusting for all covariates, higher klotho was associated with a lower prevalence of stroke [OR: 0.68, 95% CI: 0.47–0.99].ConclusionThis study disclosed the negative correlation between serum klotho protein levels and the prevalence of Stroke. Further prospective studies are requisite to investigate the impact of serum klotho protein levels on Stroke and determine the causal relationship.https://www.frontiersin.org/articles/10.3389/fneur.2025.1573027/fullklothostrokeatherosclerosiscross-sectional studyNHANES
spellingShingle Hongjia Xu
Yiming Ding
Yiming Ding
Ye Zhang
Jianwen Li
Shiyue Zhou
Dewei Wang
Xinyue Xing
Xiaoyu Ma
Cunfu Wang
Shunliang Xu
Shunliang Xu
The association between serum klotho protein and stroke: a cross-sectional study from NHANES 2007–2016
Frontiers in Neurology
klotho
stroke
atherosclerosis
cross-sectional study
NHANES
title The association between serum klotho protein and stroke: a cross-sectional study from NHANES 2007–2016
title_full The association between serum klotho protein and stroke: a cross-sectional study from NHANES 2007–2016
title_fullStr The association between serum klotho protein and stroke: a cross-sectional study from NHANES 2007–2016
title_full_unstemmed The association between serum klotho protein and stroke: a cross-sectional study from NHANES 2007–2016
title_short The association between serum klotho protein and stroke: a cross-sectional study from NHANES 2007–2016
title_sort association between serum klotho protein and stroke a cross sectional study from nhanes 2007 2016
topic klotho
stroke
atherosclerosis
cross-sectional study
NHANES
url https://www.frontiersin.org/articles/10.3389/fneur.2025.1573027/full
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