An Analysis of the Progression of Conjunctivalisation after Transplantation of Cultivated Corneal Epithelium
Purpose. To analyse the recurrence of superficial neovascularisation after previous corneal surface reconstruction with cultivated corneal epithelial cells. Materials and Methods. Forty-eight eyes underwent autologous transplantation of cultivated corneal epithelium to treat partial or total limbal...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2021/8499640 |
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| author | Dariusz Dobrowolski Boguslawa Orzechowska-Wylegala Bogumil Wowra Ewa Wróblewska-Czajka Maria Grolik Edward Wylegala |
| author_facet | Dariusz Dobrowolski Boguslawa Orzechowska-Wylegala Bogumil Wowra Ewa Wróblewska-Czajka Maria Grolik Edward Wylegala |
| author_sort | Dariusz Dobrowolski |
| collection | DOAJ |
| description | Purpose. To analyse the recurrence of superficial neovascularisation after previous corneal surface reconstruction with cultivated corneal epithelial cells. Materials and Methods. Forty-eight eyes underwent autologous transplantation of cultivated corneal epithelium to treat partial or total limbal stem cell deficiency caused by chemical or thermal injury. The carrier for the epithelial sheets was a denuded amniotic membrane. Follow-up was conducted for up to 120 months. Recurrent revascularisation (measured in terms of clock hours affected) was evaluated with slit-lamp examination and the support of confocal microscopy. Results. During the long-term observation, only 7 eyes had stable epithelia with no neovascularisation from the conjunctiva. Nineteen eyes developed pathologic vessels in 1 quadrant, with additional 4 eyes developing them in 2 quadrants. Twelve patients developed subtotal or total conjunctivalisation of the corneal surface. They were referred for second cultivated epithelium transplantation (3 patients), allogenic keratolimbal transplantation (7 patients), or keratoprosthesis (2 patients). Six patients withdrew consent. The use of confocal scans of up to 100 µm in resolution enabled the detection of pathologic microvasculature originating from the conjunctiva and the exclusion of stromal vascular ingrowth. Conclusions. Local ingrowth of the conjunctiva is a common complication after the transplantation of cultivated epithelial cells. Severe and progressive vascularisation inevitably leads to graft failure. However, if local ingrowth stops before reaching the central cornea, the treatment even with this complication can be considered a success. |
| format | Article |
| id | doaj-art-174133a7f53542819fa6af592a3183e5 |
| institution | Kabale University |
| issn | 2090-0058 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-174133a7f53542819fa6af592a3183e52025-02-03T06:05:44ZengWileyJournal of Ophthalmology2090-00582021-01-01202110.1155/2021/8499640An Analysis of the Progression of Conjunctivalisation after Transplantation of Cultivated Corneal EpitheliumDariusz Dobrowolski0Boguslawa Orzechowska-Wylegala1Bogumil Wowra2Ewa Wróblewska-Czajka3Maria Grolik4Edward Wylegala5Chair and Clinical Department of OphthalmologyENT DepartmentChair and Clinical Department of OphthalmologyChair and Clinical Department of OphthalmologyChair and Clinical Department of OphthalmologyChair and Clinical Department of OphthalmologyPurpose. To analyse the recurrence of superficial neovascularisation after previous corneal surface reconstruction with cultivated corneal epithelial cells. Materials and Methods. Forty-eight eyes underwent autologous transplantation of cultivated corneal epithelium to treat partial or total limbal stem cell deficiency caused by chemical or thermal injury. The carrier for the epithelial sheets was a denuded amniotic membrane. Follow-up was conducted for up to 120 months. Recurrent revascularisation (measured in terms of clock hours affected) was evaluated with slit-lamp examination and the support of confocal microscopy. Results. During the long-term observation, only 7 eyes had stable epithelia with no neovascularisation from the conjunctiva. Nineteen eyes developed pathologic vessels in 1 quadrant, with additional 4 eyes developing them in 2 quadrants. Twelve patients developed subtotal or total conjunctivalisation of the corneal surface. They were referred for second cultivated epithelium transplantation (3 patients), allogenic keratolimbal transplantation (7 patients), or keratoprosthesis (2 patients). Six patients withdrew consent. The use of confocal scans of up to 100 µm in resolution enabled the detection of pathologic microvasculature originating from the conjunctiva and the exclusion of stromal vascular ingrowth. Conclusions. Local ingrowth of the conjunctiva is a common complication after the transplantation of cultivated epithelial cells. Severe and progressive vascularisation inevitably leads to graft failure. However, if local ingrowth stops before reaching the central cornea, the treatment even with this complication can be considered a success.http://dx.doi.org/10.1155/2021/8499640 |
| spellingShingle | Dariusz Dobrowolski Boguslawa Orzechowska-Wylegala Bogumil Wowra Ewa Wróblewska-Czajka Maria Grolik Edward Wylegala An Analysis of the Progression of Conjunctivalisation after Transplantation of Cultivated Corneal Epithelium Journal of Ophthalmology |
| title | An Analysis of the Progression of Conjunctivalisation after Transplantation of Cultivated Corneal Epithelium |
| title_full | An Analysis of the Progression of Conjunctivalisation after Transplantation of Cultivated Corneal Epithelium |
| title_fullStr | An Analysis of the Progression of Conjunctivalisation after Transplantation of Cultivated Corneal Epithelium |
| title_full_unstemmed | An Analysis of the Progression of Conjunctivalisation after Transplantation of Cultivated Corneal Epithelium |
| title_short | An Analysis of the Progression of Conjunctivalisation after Transplantation of Cultivated Corneal Epithelium |
| title_sort | analysis of the progression of conjunctivalisation after transplantation of cultivated corneal epithelium |
| url | http://dx.doi.org/10.1155/2021/8499640 |
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