Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cells
The purpose of the study was to demonstrate how soybean peptides (SBP) protect against H2O2 -induced injury in intestinal porcine epithelial cells (IPEC-J2). SBP were prepared by protease hydrolysis, in which the molecular weights of 95.76% SBP were smaller than 3 kDa. Cell experiment included four...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2024.1487774/full |
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| author | Fushan Lu Fushan Lu Qingqiang Yin Xiao Wang Lijuan Xu Mengjie Liu Qian Zhang Hai Sheng Chaoqi Liu Ping Wang Lijun Wang Sanjun Jin Xinxin Li Juan Chang Qun Zhu |
| author_facet | Fushan Lu Fushan Lu Qingqiang Yin Xiao Wang Lijuan Xu Mengjie Liu Qian Zhang Hai Sheng Chaoqi Liu Ping Wang Lijun Wang Sanjun Jin Xinxin Li Juan Chang Qun Zhu |
| author_sort | Fushan Lu |
| collection | DOAJ |
| description | The purpose of the study was to demonstrate how soybean peptides (SBP) protect against H2O2 -induced injury in intestinal porcine epithelial cells (IPEC-J2). SBP were prepared by protease hydrolysis, in which the molecular weights of 95.76% SBP were smaller than 3 kDa. Cell experiment included four groups: Control group (IPEC-J2 cells were treated with HGDMEM), SBP group (100 μg/mL SBP incubation for 13 h), H2O2 treatment group (1 mM H2O2 treatment for 1 h), SBP + H2O2 group (100 μg/mL SBP pretreatment for 12 h followed by 1 mM H2O2 treatment for 1 h). This study showed that that treatment with single 1 mM H2O2 for 1 h significantly reduced cell viability to 52.99% (p < 0.05), up-regulated Bax and Caspase-3 gene expressions (p < 0.05), and down-regulated gene expressions of ZO-1, CAT, SOD1, HO-1 and Nrf2 (p < 0.05), compared with the control group. However, pretreatment with SBP followed by H2O2 inducement significantly increased cell viability to 72.99%, decreased cell apoptosis, increased SOD, CAT and GSH-Px activity (p < 0.05), down-regulated Bax and Caspase-3 gene expressions (p < 0.05), and up-regulated the gene expressions of ZO-1, Claudin-1, Occludin, catalase, glutathione GPX1, SOD1, HO-1, NQO1 and Nrf2, compared with the single H2O2–induced cells. According to the study, SBP pretreatment reduced H2O2-induced oxidative stress in cells and preserved the integrity of intestinal cells. |
| format | Article |
| id | doaj-art-17367b80dd7b42e3b3d8a25c5fdb4475 |
| institution | DOAJ |
| issn | 2571-581X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Sustainable Food Systems |
| spelling | doaj-art-17367b80dd7b42e3b3d8a25c5fdb44752025-08-20T02:50:04ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2024-12-01810.3389/fsufs.2024.14877741487774Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cellsFushan Lu0Fushan Lu1Qingqiang Yin2Xiao Wang3Lijuan Xu4Mengjie Liu5Qian Zhang6Hai Sheng7Chaoqi Liu8Ping Wang9Lijun Wang10Sanjun Jin11Xinxin Li12Juan Chang13Qun Zhu14College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaHenan PUAI Feed Co., Ltd., Zhoukou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaHenan PUAI Feed Co., Ltd., Zhoukou, ChinaHenan PUAI Feed Co., Ltd., Zhoukou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaHenan Delin Biological Product Co., Ltd., Xinxiang, ChinaThe purpose of the study was to demonstrate how soybean peptides (SBP) protect against H2O2 -induced injury in intestinal porcine epithelial cells (IPEC-J2). SBP were prepared by protease hydrolysis, in which the molecular weights of 95.76% SBP were smaller than 3 kDa. Cell experiment included four groups: Control group (IPEC-J2 cells were treated with HGDMEM), SBP group (100 μg/mL SBP incubation for 13 h), H2O2 treatment group (1 mM H2O2 treatment for 1 h), SBP + H2O2 group (100 μg/mL SBP pretreatment for 12 h followed by 1 mM H2O2 treatment for 1 h). This study showed that that treatment with single 1 mM H2O2 for 1 h significantly reduced cell viability to 52.99% (p < 0.05), up-regulated Bax and Caspase-3 gene expressions (p < 0.05), and down-regulated gene expressions of ZO-1, CAT, SOD1, HO-1 and Nrf2 (p < 0.05), compared with the control group. However, pretreatment with SBP followed by H2O2 inducement significantly increased cell viability to 72.99%, decreased cell apoptosis, increased SOD, CAT and GSH-Px activity (p < 0.05), down-regulated Bax and Caspase-3 gene expressions (p < 0.05), and up-regulated the gene expressions of ZO-1, Claudin-1, Occludin, catalase, glutathione GPX1, SOD1, HO-1, NQO1 and Nrf2, compared with the single H2O2–induced cells. According to the study, SBP pretreatment reduced H2O2-induced oxidative stress in cells and preserved the integrity of intestinal cells.https://www.frontiersin.org/articles/10.3389/fsufs.2024.1487774/fullsoybean peptidesoxidative stressIPEC-J2 cellsantioxidant capacitygene expressions |
| spellingShingle | Fushan Lu Fushan Lu Qingqiang Yin Xiao Wang Lijuan Xu Mengjie Liu Qian Zhang Hai Sheng Chaoqi Liu Ping Wang Lijun Wang Sanjun Jin Xinxin Li Juan Chang Qun Zhu Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cells Frontiers in Sustainable Food Systems soybean peptides oxidative stress IPEC-J2 cells antioxidant capacity gene expressions |
| title | Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cells |
| title_full | Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cells |
| title_fullStr | Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cells |
| title_full_unstemmed | Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cells |
| title_short | Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cells |
| title_sort | protective effects of soybean peptides on h2o2 induced oxidative injury in ipec j2 cells |
| topic | soybean peptides oxidative stress IPEC-J2 cells antioxidant capacity gene expressions |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2024.1487774/full |
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