Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against <i>Echinococcus granulosus</i> in Mice
<b>Background:</b> <i>Echinococcus granulosus</i> represents a significant threat to animal husbandry and human health, but its consequences are often underestimated. Vaccination can prevent <i>E. granulosus</i> infection. We investigated the immune protective eff...
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MDPI AG
2025-03-01
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| author | Yongxue Lv Jing Tang Tao Li Yinqi Zhao Changyou Wu Wei Zhao |
| author_facet | Yongxue Lv Jing Tang Tao Li Yinqi Zhao Changyou Wu Wei Zhao |
| author_sort | Yongxue Lv |
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| description | <b>Background:</b> <i>Echinococcus granulosus</i> represents a significant threat to animal husbandry and human health, but its consequences are often underestimated. Vaccination can prevent <i>E. granulosus</i> infection. We investigated the immune protective effect induced by the recombinant protein P29 of <i>E. granulosus</i> (rEg.P29) peptide vaccine. <b>Methods</b>: The CD4<sup>+</sup> T-, CD8<sup>+</sup> T-, Treg-, and CD8<sup>+</sup>CD107a<sup>+</sup> T-cell proportions in the spleen and peripheral blood of infected mice were analyzed using flow cytometry. Additionally, we measured the proportions of IFN-γ and IL-2 secreted by memory T cells, CD19<sup>+</sup>CD138<sup>−</sup>B cells, CD19<sup>+</sup>CD138<sup>+</sup> plasmablasts, CD19<sup>−</sup>CD138<sup>+</sup> plasma cells, and CD19<sup>+</sup>IgD<sup>−</sup>IgG<sup>+</sup> and CD19<sup>+</sup>IgD<sup>−</sup>IgA<sup>+</sup> memory B cells. <b>Results</b>: No significant differences were noted in CD4<sup>+</sup> T-, CD8<sup>+</sup> T-, and CD8<sup>+</sup>CD107a<sup>+</sup> Treg-cell percentages among the experimental groups. However, IFN-γ, IL-2, and TNF-α levels and vaccine-specific antibody concentrations in the plasma were significantly elevated in the rEg.P29<sub>T+B</sub> + CpG + infection and rEg.P29 + CpG + infection groups compared to those in the PBS + infection and CpG + infection groups. Similarly, CD19<sup>−</sup>CD138<sup>+</sup> plasma cell and CD19<sup>+</sup>IgD<sup>−</sup>IgG<sup>+</sup> and CD19<sup>+</sup>IgD<sup>−</sup>IgA<sup>+</sup> memory B-cell populations, along with specific antibodies, were significantly higher in these groups. Especially, the average cyst burden in the rEg.P29<sub>T+B</sub> + CpG + infection and rEg.P29 + CpG + infection groups was significantly reduced compared to that in the PBS + infection and CpG + infection groups. <b>Conclusions</b>: Synthetic peptide vaccines targeting rEg.P29 can effectively inhibit cysts, offering a novel strategy for the development of vaccines against <i>E. granulosus</i>. These findings provide a foundation for further research on the immunogenicity and protective efficacy of rEg.P29-based vaccines. |
| format | Article |
| id | doaj-art-1735c9cf386748adbac63a8bb6aa7574 |
| institution | OA Journals |
| issn | 2076-393X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-1735c9cf386748adbac63a8bb6aa75742025-08-20T01:50:07ZengMDPI AGVaccines2076-393X2025-03-0113326610.3390/vaccines13030266Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against <i>Echinococcus granulosus</i> in MiceYongxue Lv0Jing Tang1Tao Li2Yinqi Zhao3Changyou Wu4Wei Zhao5School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Basic Medicine, Ningxia Medical University, Yinchuan 750004, ChinaDepartment of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, ChinaNingxia Key Laboratory of Prevention and Control of Common Infectious Diseases, Ningxia Hui Autonomous Region, Yinchuan 750004, ChinaInstitute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 5102275, ChinaSchool of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China<b>Background:</b> <i>Echinococcus granulosus</i> represents a significant threat to animal husbandry and human health, but its consequences are often underestimated. Vaccination can prevent <i>E. granulosus</i> infection. We investigated the immune protective effect induced by the recombinant protein P29 of <i>E. granulosus</i> (rEg.P29) peptide vaccine. <b>Methods</b>: The CD4<sup>+</sup> T-, CD8<sup>+</sup> T-, Treg-, and CD8<sup>+</sup>CD107a<sup>+</sup> T-cell proportions in the spleen and peripheral blood of infected mice were analyzed using flow cytometry. Additionally, we measured the proportions of IFN-γ and IL-2 secreted by memory T cells, CD19<sup>+</sup>CD138<sup>−</sup>B cells, CD19<sup>+</sup>CD138<sup>+</sup> plasmablasts, CD19<sup>−</sup>CD138<sup>+</sup> plasma cells, and CD19<sup>+</sup>IgD<sup>−</sup>IgG<sup>+</sup> and CD19<sup>+</sup>IgD<sup>−</sup>IgA<sup>+</sup> memory B cells. <b>Results</b>: No significant differences were noted in CD4<sup>+</sup> T-, CD8<sup>+</sup> T-, and CD8<sup>+</sup>CD107a<sup>+</sup> Treg-cell percentages among the experimental groups. However, IFN-γ, IL-2, and TNF-α levels and vaccine-specific antibody concentrations in the plasma were significantly elevated in the rEg.P29<sub>T+B</sub> + CpG + infection and rEg.P29 + CpG + infection groups compared to those in the PBS + infection and CpG + infection groups. Similarly, CD19<sup>−</sup>CD138<sup>+</sup> plasma cell and CD19<sup>+</sup>IgD<sup>−</sup>IgG<sup>+</sup> and CD19<sup>+</sup>IgD<sup>−</sup>IgA<sup>+</sup> memory B-cell populations, along with specific antibodies, were significantly higher in these groups. Especially, the average cyst burden in the rEg.P29<sub>T+B</sub> + CpG + infection and rEg.P29 + CpG + infection groups was significantly reduced compared to that in the PBS + infection and CpG + infection groups. <b>Conclusions</b>: Synthetic peptide vaccines targeting rEg.P29 can effectively inhibit cysts, offering a novel strategy for the development of vaccines against <i>E. granulosus</i>. These findings provide a foundation for further research on the immunogenicity and protective efficacy of rEg.P29-based vaccines.https://www.mdpi.com/2076-393X/13/3/266<i>Echinococcus granulosus</i>rEg.P29synthetic peptides vaccineprotective effect |
| spellingShingle | Yongxue Lv Jing Tang Tao Li Yinqi Zhao Changyou Wu Wei Zhao Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against <i>Echinococcus granulosus</i> in Mice Vaccines <i>Echinococcus granulosus</i> rEg.P29 synthetic peptides vaccine protective effect |
| title | Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against <i>Echinococcus granulosus</i> in Mice |
| title_full | Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against <i>Echinococcus granulosus</i> in Mice |
| title_fullStr | Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against <i>Echinococcus granulosus</i> in Mice |
| title_full_unstemmed | Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against <i>Echinococcus granulosus</i> in Mice |
| title_short | Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against <i>Echinococcus granulosus</i> in Mice |
| title_sort | synthetic reg p29 peptides induce protective immune responses against i echinococcus granulosus i in mice |
| topic | <i>Echinococcus granulosus</i> rEg.P29 synthetic peptides vaccine protective effect |
| url | https://www.mdpi.com/2076-393X/13/3/266 |
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