Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal Cells
Objective. Cytokines and chemokines are widely involved in cancer cell progression and thus represent promising candidate factors for new biomarkers. Methods. Four renal cell cancer (RCC) cell lines (Caki-1, 786-O, RCC4, and A498) and a nonmalignant renal cell line (RC-124) were examined with respec...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2017/7190546 |
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| author | Nadine Gelbrich Hannes Ahrend Anne Kaul Lars-Ove Brandenburg Uwe Zimmermann Alexander Mustea Martin Burchardt Denis Gümbel Matthias B. Stope |
| author_facet | Nadine Gelbrich Hannes Ahrend Anne Kaul Lars-Ove Brandenburg Uwe Zimmermann Alexander Mustea Martin Burchardt Denis Gümbel Matthias B. Stope |
| author_sort | Nadine Gelbrich |
| collection | DOAJ |
| description | Objective. Cytokines and chemokines are widely involved in cancer cell progression and thus represent promising candidate factors for new biomarkers. Methods. Four renal cell cancer (RCC) cell lines (Caki-1, 786-O, RCC4, and A498) and a nonmalignant renal cell line (RC-124) were examined with respect to their proliferation. The cytokine and chemokine expression pattern was examined by a DNA array (Human Cytokines & Chemokines RT2 Profiler PCR Array; Qiagen, Hilden, Germany), and expression profiles were compared. Results. Caki-1 and 786-O cells exhibited significantly increased proliferation rates, whereas RCC4 and A498 cells demonstrated attenuated proliferation, compared to nonmalignant RC-124 cells. Expression analysis revealed 52 cytokines and chemokines primarily involved in proliferation and inflammation and differentially expressed not only in malignant and nonmalignant renal cells but also in the four RCC cell lines. Conclusion. This is the first study examining the expression of 84 cytokines and chemokines in four RCC cell lines compared to that in a nonmalignant renal cell line. VEGFA, NODAL, and BMP6 correlated with RCC cell line proliferation and, thus, may represent putative clinical biomarkers for RCC progression as well as for RCC diagnosis and prognosis. |
| format | Article |
| id | doaj-art-172031f2f09f4c44a8b17c2ef5834fba |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-172031f2f09f4c44a8b17c2ef5834fba2025-08-20T03:54:33ZengWileyAnalytical Cellular Pathology2210-71772210-71852017-01-01201710.1155/2017/71905467190546Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal CellsNadine Gelbrich0Hannes Ahrend1Anne Kaul2Lars-Ove Brandenburg3Uwe Zimmermann4Alexander Mustea5Martin Burchardt6Denis Gümbel7Matthias B. Stope8Department of Urology, University Medicine Greifswald, Greifswald, GermanyDepartment of Urology, University Medicine Greifswald, Greifswald, GermanyDepartment of Gynaecology and Obstetrics, University Medicine Greifswald, Greifswald, GermanyDepartment of Anatomy and Cell Biology, RWTH Aachen University, Aachen, GermanyDepartment of Urology, University Medicine Greifswald, Greifswald, GermanyDepartment of Gynaecology and Obstetrics, University Medicine Greifswald, Greifswald, GermanyDepartment of Urology, University Medicine Greifswald, Greifswald, GermanyDepartment of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, GermanyDepartment of Urology, University Medicine Greifswald, Greifswald, GermanyObjective. Cytokines and chemokines are widely involved in cancer cell progression and thus represent promising candidate factors for new biomarkers. Methods. Four renal cell cancer (RCC) cell lines (Caki-1, 786-O, RCC4, and A498) and a nonmalignant renal cell line (RC-124) were examined with respect to their proliferation. The cytokine and chemokine expression pattern was examined by a DNA array (Human Cytokines & Chemokines RT2 Profiler PCR Array; Qiagen, Hilden, Germany), and expression profiles were compared. Results. Caki-1 and 786-O cells exhibited significantly increased proliferation rates, whereas RCC4 and A498 cells demonstrated attenuated proliferation, compared to nonmalignant RC-124 cells. Expression analysis revealed 52 cytokines and chemokines primarily involved in proliferation and inflammation and differentially expressed not only in malignant and nonmalignant renal cells but also in the four RCC cell lines. Conclusion. This is the first study examining the expression of 84 cytokines and chemokines in four RCC cell lines compared to that in a nonmalignant renal cell line. VEGFA, NODAL, and BMP6 correlated with RCC cell line proliferation and, thus, may represent putative clinical biomarkers for RCC progression as well as for RCC diagnosis and prognosis.http://dx.doi.org/10.1155/2017/7190546 |
| spellingShingle | Nadine Gelbrich Hannes Ahrend Anne Kaul Lars-Ove Brandenburg Uwe Zimmermann Alexander Mustea Martin Burchardt Denis Gümbel Matthias B. Stope Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal Cells Analytical Cellular Pathology |
| title | Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal Cells |
| title_full | Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal Cells |
| title_fullStr | Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal Cells |
| title_full_unstemmed | Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal Cells |
| title_short | Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal Cells |
| title_sort | different cytokine and chemokine expression patterns in malignant compared to those in nonmalignant renal cells |
| url | http://dx.doi.org/10.1155/2017/7190546 |
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