Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water
A rapid detection method for the pesticide pentachlorophenol (PCP) — polarization fluoroimmunoassay (PFIA) — in the dynamic range of 10–9,000 ppb was developed. PCP may form polychlorinated dibenzo-p-dioxins, making environmental monitoring of this compound an issue of great importance. In order to...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.209 |
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| author | Vitalia S. Krikounova Ramadan Abuknesha Sergei A. Eremin |
| author_facet | Vitalia S. Krikounova Ramadan Abuknesha Sergei A. Eremin |
| author_sort | Vitalia S. Krikounova |
| collection | DOAJ |
| description | A rapid detection method for the pesticide pentachlorophenol (PCP) — polarization fluoroimmunoassay (PFIA) — in the dynamic range of 10–9,000 ppb was developed. PCP may form polychlorinated dibenzo-p-dioxins, making environmental monitoring of this compound an issue of great importance. In order to optimize the PFIA procedure, a number of fluorescein-labeled PCP derivatives and similar compounds (tracers) were synthesized, and the influence of their structure on PFIA characteristics was studied. Also, two antisera were tested in developing PFIA for PCP. The developed method is highly specific for PCP and can be used for its determination in water samples at a level down to 10 ppb. Total time of the assay for 10 samples is about 7 min. The assay provides a useful and a highly practical screening tool for the processing of large numbers of samples and for the preliminary estimation of potential dioxins contamination in water resources. |
| format | Article |
| id | doaj-art-1718f818dc12487aa109cb57017a71e6 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1718f818dc12487aa109cb57017a71e62025-02-03T06:13:09ZengWileyThe Scientific World Journal1537-744X2002-01-0121132113710.1100/tsw.2002.209Express Detection of Pentachlorophenol as Dioxins Precursor in Natural WaterVitalia S. Krikounova0Ramadan Abuknesha1Sergei A. Eremin2Faculty of Chemistry, M.V. Lomonosov Moscow State University, RussiaLife Sciences, King’s College London, University of London, UKFaculty of Chemistry, M.V. Lomonosov Moscow State University, RussiaA rapid detection method for the pesticide pentachlorophenol (PCP) — polarization fluoroimmunoassay (PFIA) — in the dynamic range of 10–9,000 ppb was developed. PCP may form polychlorinated dibenzo-p-dioxins, making environmental monitoring of this compound an issue of great importance. In order to optimize the PFIA procedure, a number of fluorescein-labeled PCP derivatives and similar compounds (tracers) were synthesized, and the influence of their structure on PFIA characteristics was studied. Also, two antisera were tested in developing PFIA for PCP. The developed method is highly specific for PCP and can be used for its determination in water samples at a level down to 10 ppb. Total time of the assay for 10 samples is about 7 min. The assay provides a useful and a highly practical screening tool for the processing of large numbers of samples and for the preliminary estimation of potential dioxins contamination in water resources.http://dx.doi.org/10.1100/tsw.2002.209 |
| spellingShingle | Vitalia S. Krikounova Ramadan Abuknesha Sergei A. Eremin Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water The Scientific World Journal |
| title | Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water |
| title_full | Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water |
| title_fullStr | Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water |
| title_full_unstemmed | Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water |
| title_short | Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water |
| title_sort | express detection of pentachlorophenol as dioxins precursor in natural water |
| url | http://dx.doi.org/10.1100/tsw.2002.209 |
| work_keys_str_mv | AT vitaliaskrikounova expressdetectionofpentachlorophenolasdioxinsprecursorinnaturalwater AT ramadanabuknesha expressdetectionofpentachlorophenolasdioxinsprecursorinnaturalwater AT sergeiaeremin expressdetectionofpentachlorophenolasdioxinsprecursorinnaturalwater |