Global prevalence and antibiotic resistance profiles of carbapenem-resistant Pseudomonas aeruginosa reported from 2014 to 2024: a systematic review and meta-analysis

IntroductionCarbapenem-resistant Pseudomonas aeruginosa (CRPA) represents a global threat, but the global distribution of carbapenem resistant bacteria remains a critical issue in public health.MethodsWe conducted a systematic review and meta-analysis on the global pooled prevalence estimate (PPE) o...

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Main Authors: Tsepo Ramatla, Jane Nkhebenyane, Kgaugelo E. Lekota, Oriel Thekisoe, Maropeng Monyama, Conrad Chibunna Achilonu, George Khasapane
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1599070/full
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author Tsepo Ramatla
Jane Nkhebenyane
Kgaugelo E. Lekota
Oriel Thekisoe
Maropeng Monyama
Conrad Chibunna Achilonu
George Khasapane
author_facet Tsepo Ramatla
Jane Nkhebenyane
Kgaugelo E. Lekota
Oriel Thekisoe
Maropeng Monyama
Conrad Chibunna Achilonu
George Khasapane
author_sort Tsepo Ramatla
collection DOAJ
description IntroductionCarbapenem-resistant Pseudomonas aeruginosa (CRPA) represents a global threat, but the global distribution of carbapenem resistant bacteria remains a critical issue in public health.MethodsWe conducted a systematic review and meta-analysis on the global pooled prevalence estimate (PPE) of CRPA and their antibiotic resistance. The systematic review protocol was registered with PROSPERO (CRD42024579654). This study was carried out following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. Heterogeneity between studies was assessed using Cochrane Q test and I2 test statistics based on the random effects model. Comprehensive meta-analysis software v4.0 was used to analyze the pooled prevalence of CRPA.ResultsA total of 163 studies (both clinical and screening samples) containing a total of 58,344 cases from 39 countries were included in this study. The overall PPE of CRPA was 34.7% (95% CI: 0.316–0.37.8) for both clinical and screening samples. Meropenem had a PPE of 31.2% (95% CI: 0.272–0.352) and imipenem had the lowest PPE of 27.7% (95% CI: 0.238–0.319). Japan had the highest PPE at 98.2% (95% CI: 0.482–0.100) of CRPA, and the lowest was observed for Saudi Arabia at 13.9% (95% CI: 0. 064–0. 277). CRPA is widespread on five continents except Australia and Antarctica, while the highest PPE is in Europe at 47.6% (95% CI: 0.359–0.595) and the lowest in Asia at 32, 8% (95% CI: 0.293–0.364). The relatively higher PPE of CRPA was observed in Europe during the year interval 2014–2017 at 95.4% (95% CI: 0.388–0.999), followed by Africa from the year 2022–2024 with 38.5% (95% CI: 0.243–0.550). Ceftazidime was significantly higher in studies conducted before 2019 with a PPE of 44.7% (95% CI: 0.246% – 0.668), while CRPA after 2019 had a higher resistance to cefoperazone/sulbactam with a PPE of 17.3% (95% CI: 0.050–0.455).DiscussionThis review indicates that the prevalence of CRPA is generally high and varies significantly between countries. To prevent the emergence of CRPA and antibiotic resistance, future initiatives should prioritise strengthening laboratory capacity for early detection of antibiotic resistance.
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spelling doaj-art-1706799adf1c4dfeb9b7c0ab4a2b760d2025-08-20T03:51:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.15990701599070Global prevalence and antibiotic resistance profiles of carbapenem-resistant Pseudomonas aeruginosa reported from 2014 to 2024: a systematic review and meta-analysisTsepo Ramatla0Jane Nkhebenyane1Kgaugelo E. Lekota2Oriel Thekisoe3Maropeng Monyama4Conrad Chibunna Achilonu5George Khasapane6Centre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, Bloemfontein, South AfricaCentre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, Bloemfontein, South AfricaUnit for Environmental Sciences and Management, North-West University, Potchefstroom, South AfricaUnit for Environmental Sciences and Management, North-West University, Potchefstroom, South AfricaDepartment of Life and Consumer Sciences, University of South Africa, Johannesburg, South AfricaThoracic Diseases Research Unit, Departments of Medicine and Biochemistry, Mayo Clinic College of Medicine, Rochester, MN, United StatesCentre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, Bloemfontein, South AfricaIntroductionCarbapenem-resistant Pseudomonas aeruginosa (CRPA) represents a global threat, but the global distribution of carbapenem resistant bacteria remains a critical issue in public health.MethodsWe conducted a systematic review and meta-analysis on the global pooled prevalence estimate (PPE) of CRPA and their antibiotic resistance. The systematic review protocol was registered with PROSPERO (CRD42024579654). This study was carried out following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. Heterogeneity between studies was assessed using Cochrane Q test and I2 test statistics based on the random effects model. Comprehensive meta-analysis software v4.0 was used to analyze the pooled prevalence of CRPA.ResultsA total of 163 studies (both clinical and screening samples) containing a total of 58,344 cases from 39 countries were included in this study. The overall PPE of CRPA was 34.7% (95% CI: 0.316–0.37.8) for both clinical and screening samples. Meropenem had a PPE of 31.2% (95% CI: 0.272–0.352) and imipenem had the lowest PPE of 27.7% (95% CI: 0.238–0.319). Japan had the highest PPE at 98.2% (95% CI: 0.482–0.100) of CRPA, and the lowest was observed for Saudi Arabia at 13.9% (95% CI: 0. 064–0. 277). CRPA is widespread on five continents except Australia and Antarctica, while the highest PPE is in Europe at 47.6% (95% CI: 0.359–0.595) and the lowest in Asia at 32, 8% (95% CI: 0.293–0.364). The relatively higher PPE of CRPA was observed in Europe during the year interval 2014–2017 at 95.4% (95% CI: 0.388–0.999), followed by Africa from the year 2022–2024 with 38.5% (95% CI: 0.243–0.550). Ceftazidime was significantly higher in studies conducted before 2019 with a PPE of 44.7% (95% CI: 0.246% – 0.668), while CRPA after 2019 had a higher resistance to cefoperazone/sulbactam with a PPE of 17.3% (95% CI: 0.050–0.455).DiscussionThis review indicates that the prevalence of CRPA is generally high and varies significantly between countries. To prevent the emergence of CRPA and antibiotic resistance, future initiatives should prioritise strengthening laboratory capacity for early detection of antibiotic resistance.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1599070/fullpooled prevalence estimateCRPAantibiotic resistanceglobalmeta-analysis
spellingShingle Tsepo Ramatla
Jane Nkhebenyane
Kgaugelo E. Lekota
Oriel Thekisoe
Maropeng Monyama
Conrad Chibunna Achilonu
George Khasapane
Global prevalence and antibiotic resistance profiles of carbapenem-resistant Pseudomonas aeruginosa reported from 2014 to 2024: a systematic review and meta-analysis
Frontiers in Microbiology
pooled prevalence estimate
CRPA
antibiotic resistance
global
meta-analysis
title Global prevalence and antibiotic resistance profiles of carbapenem-resistant Pseudomonas aeruginosa reported from 2014 to 2024: a systematic review and meta-analysis
title_full Global prevalence and antibiotic resistance profiles of carbapenem-resistant Pseudomonas aeruginosa reported from 2014 to 2024: a systematic review and meta-analysis
title_fullStr Global prevalence and antibiotic resistance profiles of carbapenem-resistant Pseudomonas aeruginosa reported from 2014 to 2024: a systematic review and meta-analysis
title_full_unstemmed Global prevalence and antibiotic resistance profiles of carbapenem-resistant Pseudomonas aeruginosa reported from 2014 to 2024: a systematic review and meta-analysis
title_short Global prevalence and antibiotic resistance profiles of carbapenem-resistant Pseudomonas aeruginosa reported from 2014 to 2024: a systematic review and meta-analysis
title_sort global prevalence and antibiotic resistance profiles of carbapenem resistant pseudomonas aeruginosa reported from 2014 to 2024 a systematic review and meta analysis
topic pooled prevalence estimate
CRPA
antibiotic resistance
global
meta-analysis
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1599070/full
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