Selective ligand recognition by a diversity-generating retroelement variable protein.
Diversity-generating retroelements (DGRs) recognize novel ligands through massive protein sequence variation, a property shared uniquely with the adaptive immune response. Little is known about how recognition is achieved by DGR variable proteins. Here, we present the structure of the Bordetella bac...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-06-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060131&type=printable |
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| _version_ | 1850234091723554816 |
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| author | Jason L Miller Johanne Le Coq Asher Hodes Roman Barbalat Jeff F Miller Partho Ghosh |
| author_facet | Jason L Miller Johanne Le Coq Asher Hodes Roman Barbalat Jeff F Miller Partho Ghosh |
| author_sort | Jason L Miller |
| collection | DOAJ |
| description | Diversity-generating retroelements (DGRs) recognize novel ligands through massive protein sequence variation, a property shared uniquely with the adaptive immune response. Little is known about how recognition is achieved by DGR variable proteins. Here, we present the structure of the Bordetella bacteriophage DGR variable protein major tropism determinant (Mtd) bound to the receptor pertactin, revealing remarkable adaptability in the static binding sites of Mtd. Despite large dissimilarities in ligand binding mode, principles underlying selective recognition were strikingly conserved between Mtd and immunoreceptors. Central to this was the differential amplification of binding strengths by avidity (i.e., multivalency), which not only relaxed the demand for optimal complementarity between Mtd and pertactin but also enhanced distinctions among binding events to provide selectivity. A quantitatively similar balance between complementarity and avidity was observed for Bordetella bacteriophage DGR as occurs in the immune system, suggesting that variable repertoires operate under a narrow set of conditions to recognize novel ligands. |
| format | Article |
| id | doaj-art-1705d6f2babb4c68b4ef80c4f69442e3 |
| institution | OA Journals |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2008-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-1705d6f2babb4c68b4ef80c4f69442e32025-08-20T02:02:44ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852008-06-0166e13110.1371/journal.pbio.0060131Selective ligand recognition by a diversity-generating retroelement variable protein.Jason L MillerJohanne Le CoqAsher HodesRoman BarbalatJeff F MillerPartho GhoshDiversity-generating retroelements (DGRs) recognize novel ligands through massive protein sequence variation, a property shared uniquely with the adaptive immune response. Little is known about how recognition is achieved by DGR variable proteins. Here, we present the structure of the Bordetella bacteriophage DGR variable protein major tropism determinant (Mtd) bound to the receptor pertactin, revealing remarkable adaptability in the static binding sites of Mtd. Despite large dissimilarities in ligand binding mode, principles underlying selective recognition were strikingly conserved between Mtd and immunoreceptors. Central to this was the differential amplification of binding strengths by avidity (i.e., multivalency), which not only relaxed the demand for optimal complementarity between Mtd and pertactin but also enhanced distinctions among binding events to provide selectivity. A quantitatively similar balance between complementarity and avidity was observed for Bordetella bacteriophage DGR as occurs in the immune system, suggesting that variable repertoires operate under a narrow set of conditions to recognize novel ligands.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060131&type=printable |
| spellingShingle | Jason L Miller Johanne Le Coq Asher Hodes Roman Barbalat Jeff F Miller Partho Ghosh Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS Biology |
| title | Selective ligand recognition by a diversity-generating retroelement variable protein. |
| title_full | Selective ligand recognition by a diversity-generating retroelement variable protein. |
| title_fullStr | Selective ligand recognition by a diversity-generating retroelement variable protein. |
| title_full_unstemmed | Selective ligand recognition by a diversity-generating retroelement variable protein. |
| title_short | Selective ligand recognition by a diversity-generating retroelement variable protein. |
| title_sort | selective ligand recognition by a diversity generating retroelement variable protein |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060131&type=printable |
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