Clinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemic

Aim. To determine the presence or absence of specific IgG antibodies against SARS-CoV-2 antigens in outpatient clinic staff and to compare clinical and immunological features from April to August 2020.Materials and Methods. The control group comprised 386 employees of the Novosibirsk City Clinical P...

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Main Authors: O. O. Obukhova, A. N. Trunov, O. M. Gorbenko, A. P. Shvayuk, T. I. Ryabichenko, G A. Skosyreva, A. V. Obukhov
Format: Article
Language:Russian
Published: Kamerton 2021-01-01
Series:Юг России: экология, развитие
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Online Access:https://ecodag.elpub.ru/ugro/article/view/2060
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author O. O. Obukhova
A. N. Trunov
O. M. Gorbenko
A. P. Shvayuk
T. I. Ryabichenko
G A. Skosyreva
A. V. Obukhov
author_facet O. O. Obukhova
A. N. Trunov
O. M. Gorbenko
A. P. Shvayuk
T. I. Ryabichenko
G A. Skosyreva
A. V. Obukhov
author_sort O. O. Obukhova
collection DOAJ
description Aim. To determine the presence or absence of specific IgG antibodies against SARS-CoV-2 antigens in outpatient clinic staff and to compare clinical and immunological features from April to August 2020.Materials and Methods. The control group comprised 386 employees of the Novosibirsk City Clinical Polyclinic №13.The determination of IgG antibodies against SARS-CoV-2 antigens in blood serum was performed by using the ELISA method. A real time method of reverse transcription and polymerase chain reaction was used to extract RNA SARS-CoV-2 from nasopharyngeal and oropharyngeal swabs.Results. Specific IgG antibodies against SARS-CoV-2 antigens were detected in 32 (8.42%) employees of the polyclinic. Antibodies were not detected in 91.58% of employees. 9 members (28.12%) had clinical symptoms of varying degrees of disease severity in the group of employees with the presence of antibodies, 4 members of this group (12,51%) had bilateral community-acquired pneumonia with signs of COVID infection, another 5 members (15.61%) with antibodies had signs of ARVI of mild and moderate severity. RNA SARS-CoV-2 in the group of employees with the presence of antibodies and clinical implications was not detected in any case; 23 (71.88%) members with the presence of IgG-antibodies did not have clinical implications of the disease.Conclusion. The presence of specific antibodies against SARS-CoV-2 in employees with clinical implications of COVID-19 without detection of the RNA virus in the biological material is a retrospective confirmation of the etiology of the transferred infection. The detection of specific antibodies in persons who did not have clinical implications can serve as confirmation of the asymptomatic course of the transferred coronavirus infection.
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spelling doaj-art-16ec0cf4a74348749ade55dd8dcd13f22025-08-20T03:00:05ZrusKamertonЮг России: экология, развитие1992-10982413-09582021-01-0115416116710.18470/1992-1098-2020-4-161-1671146Clinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemicO. O. Obukhova0A. N. Trunov1O. M. Gorbenko2A. P. Shvayuk3T. I. Ryabichenko4G A. Skosyreva5A. V. Obukhov6Federal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineFederal Research Centre of Fundamental and Translational MedicineCity Clinical Polyclinic №13Aim. To determine the presence or absence of specific IgG antibodies against SARS-CoV-2 antigens in outpatient clinic staff and to compare clinical and immunological features from April to August 2020.Materials and Methods. The control group comprised 386 employees of the Novosibirsk City Clinical Polyclinic №13.The determination of IgG antibodies against SARS-CoV-2 antigens in blood serum was performed by using the ELISA method. A real time method of reverse transcription and polymerase chain reaction was used to extract RNA SARS-CoV-2 from nasopharyngeal and oropharyngeal swabs.Results. Specific IgG antibodies against SARS-CoV-2 antigens were detected in 32 (8.42%) employees of the polyclinic. Antibodies were not detected in 91.58% of employees. 9 members (28.12%) had clinical symptoms of varying degrees of disease severity in the group of employees with the presence of antibodies, 4 members of this group (12,51%) had bilateral community-acquired pneumonia with signs of COVID infection, another 5 members (15.61%) with antibodies had signs of ARVI of mild and moderate severity. RNA SARS-CoV-2 in the group of employees with the presence of antibodies and clinical implications was not detected in any case; 23 (71.88%) members with the presence of IgG-antibodies did not have clinical implications of the disease.Conclusion. The presence of specific antibodies against SARS-CoV-2 in employees with clinical implications of COVID-19 without detection of the RNA virus in the biological material is a retrospective confirmation of the etiology of the transferred infection. The detection of specific antibodies in persons who did not have clinical implications can serve as confirmation of the asymptomatic course of the transferred coronavirus infection.https://ecodag.elpub.ru/ugro/article/view/2060coronavirussars-cov-2antibodiespeopleimmunitymedical staffoutpatient care
spellingShingle O. O. Obukhova
A. N. Trunov
O. M. Gorbenko
A. P. Shvayuk
T. I. Ryabichenko
G A. Skosyreva
A. V. Obukhov
Clinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemic
Юг России: экология, развитие
coronavirus
sars-cov-2
antibodies
people
immunity
medical staff
outpatient care
title Clinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemic
title_full Clinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemic
title_fullStr Clinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemic
title_full_unstemmed Clinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemic
title_short Clinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemic
title_sort clinical and immunological comparisons of the results of testing for covid 19 of employees of an outpatient medical organization of a large industrial centre during a pandemic
topic coronavirus
sars-cov-2
antibodies
people
immunity
medical staff
outpatient care
url https://ecodag.elpub.ru/ugro/article/view/2060
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