MGAT4EP promotes tumor progression and serves as a prognostic marker for breast cancer
Breast cancer remains a global health challenge with varied prognoses despite treatment advancements. Therefore, this study explores the pseudogene MGAT4EP as a potential biomarker and therapeutic target in breast cancer. Using TCGA data and bioinformatics, MGAT4EP was identified as significantly ov...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Cancer Biology & Therapy |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/15384047.2025.2475604 |
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| _version_ | 1849762300556214272 |
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| author | Lin Zhong Jianfeng Zhu Jie Chen Xuchu Jin Liangquan Liu Shufeng Ji Jing Luo Hong Wang |
| author_facet | Lin Zhong Jianfeng Zhu Jie Chen Xuchu Jin Liangquan Liu Shufeng Ji Jing Luo Hong Wang |
| author_sort | Lin Zhong |
| collection | DOAJ |
| description | Breast cancer remains a global health challenge with varied prognoses despite treatment advancements. Therefore, this study explores the pseudogene MGAT4EP as a potential biomarker and therapeutic target in breast cancer. Using TCGA data and bioinformatics, MGAT4EP was identified as significantly overexpressed in breast cancer tissues and associated with poor prognosis. Multivariate Cox regression confirmed MGAT4EP as important prognostic factor. A clinical prediction model based on MGAT4EP expression showed high accuracy for 1-, 3-, and 5-year survival rates and was translated into a nomogram for clinical application. Functional studies revealed that silencing MGAT4EP via siRNA promoted apoptosis, inhibited migration and invasion in breast cancer cells. RNA-seq, GSEA, and GO analyses linked MGAT4EP to apoptosis and focal adhesion pathways. Notably, knock down of MGAT4EP significantly suppressed tumor growth and metastasis in xenograft and lung metastasis models. Taken together, these findings establish MGAT4EP as an attractive target for metastatic breast cancer and provide a potential a promising therapeutic target for breast cancer treatment. |
| format | Article |
| id | doaj-art-16e345716df04d669196f0c0f2c3d2c3 |
| institution | DOAJ |
| issn | 1538-4047 1555-8576 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cancer Biology & Therapy |
| spelling | doaj-art-16e345716df04d669196f0c0f2c3d2c32025-08-20T03:05:46ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762025-12-0126110.1080/15384047.2025.2475604MGAT4EP promotes tumor progression and serves as a prognostic marker for breast cancerLin Zhong0Jianfeng Zhu1Jie Chen2Xuchu Jin3Liangquan Liu4Shufeng Ji5Jing Luo6Hong Wang7Department of Breast Surgery, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, ChinaDepartment of Breast Surgery, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, ChinaDepartment of Breast Surgery, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, ChinaDepartment of Breast Surgery, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, ChinaDepartment of Breast Surgery, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, ChinaDepartment of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Breast Surgery, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, ChinaSchool of Pharmacy, Sun Yat-sen University, Guangzhou, Guangdong, ChinaBreast cancer remains a global health challenge with varied prognoses despite treatment advancements. Therefore, this study explores the pseudogene MGAT4EP as a potential biomarker and therapeutic target in breast cancer. Using TCGA data and bioinformatics, MGAT4EP was identified as significantly overexpressed in breast cancer tissues and associated with poor prognosis. Multivariate Cox regression confirmed MGAT4EP as important prognostic factor. A clinical prediction model based on MGAT4EP expression showed high accuracy for 1-, 3-, and 5-year survival rates and was translated into a nomogram for clinical application. Functional studies revealed that silencing MGAT4EP via siRNA promoted apoptosis, inhibited migration and invasion in breast cancer cells. RNA-seq, GSEA, and GO analyses linked MGAT4EP to apoptosis and focal adhesion pathways. Notably, knock down of MGAT4EP significantly suppressed tumor growth and metastasis in xenograft and lung metastasis models. Taken together, these findings establish MGAT4EP as an attractive target for metastatic breast cancer and provide a potential a promising therapeutic target for breast cancer treatment.https://www.tandfonline.com/doi/10.1080/15384047.2025.2475604Breast cancerMGAT4EPtumor progressionpoor prognosis |
| spellingShingle | Lin Zhong Jianfeng Zhu Jie Chen Xuchu Jin Liangquan Liu Shufeng Ji Jing Luo Hong Wang MGAT4EP promotes tumor progression and serves as a prognostic marker for breast cancer Cancer Biology & Therapy Breast cancer MGAT4EP tumor progression poor prognosis |
| title | MGAT4EP promotes tumor progression and serves as a prognostic marker for breast cancer |
| title_full | MGAT4EP promotes tumor progression and serves as a prognostic marker for breast cancer |
| title_fullStr | MGAT4EP promotes tumor progression and serves as a prognostic marker for breast cancer |
| title_full_unstemmed | MGAT4EP promotes tumor progression and serves as a prognostic marker for breast cancer |
| title_short | MGAT4EP promotes tumor progression and serves as a prognostic marker for breast cancer |
| title_sort | mgat4ep promotes tumor progression and serves as a prognostic marker for breast cancer |
| topic | Breast cancer MGAT4EP tumor progression poor prognosis |
| url | https://www.tandfonline.com/doi/10.1080/15384047.2025.2475604 |
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