Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin
IntroductionThe soybean 7S globulin is a major allergen responsible for diarrhea in weaned piglets, leading to significant economic loss in the pig breeding industry. Therefore, there is an urgent need to find effective and safe therapeutic agents to prevent and treat diarrhea caused by soybean 7S g...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-03-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1548866/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849772952209326080 |
|---|---|
| author | Youtian Deng Xiaoli Wu Yingying Wan Junliang Deng Junliang Deng Huidan Deng Huidan Deng |
| author_facet | Youtian Deng Xiaoli Wu Yingying Wan Junliang Deng Junliang Deng Huidan Deng Huidan Deng |
| author_sort | Youtian Deng |
| collection | DOAJ |
| description | IntroductionThe soybean 7S globulin is a major allergen responsible for diarrhea in weaned piglets, leading to significant economic loss in the pig breeding industry. Therefore, there is an urgent need to find effective and safe therapeutic agents to prevent and treat diarrhea caused by soybean 7S globulin. Given the global trend toward “antibiotic alternatives,” traditional Chinese medicines (TCMs) offer a promising approach for the prevention and control of animal diseases.MethodsIn this study, four TCM monomers (curcumin, eleutheroside E, saponin B4, and forsythia A) were evaluated for their protective and therapeutic effects on intestinal epithelial cells (IPEC-J2) damaged by soybean 7S globulin. The CCK8 assay, western blot assay, Elisa assay, and PCR assay were used in this study.ResultsThe results demonstrated that curcumin at concentrations of 0.02, 0.04, and 0.08 μg/mL, eleutheroside E at 25, and 50 μg/mL, saponin B4 at 12.5, 25, and 50 μg/mL, and forsythia A at 20, and 40 μg/mL had significant ameliorative effects on cell viability, permeability, and integrity. Furthermore, the TCM monomers alleviated the inflammatory response, reduced the disruption of tight junctions, and improved the cellular mechanical barrier. These protective effects were likely mediated through the inhibition of the Rho/ROCK signaling pathway, characterized by down regulation of RhoA, ROCK1, ROCK2, and MLKC expression.DiscussionThese findings suggest that the four TCM monomers have the potential to treat diarrhea of weaned piglets caused by soybean protein. |
| format | Article |
| id | doaj-art-16e26af027ff4a919d6c8ee622e2fe64 |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-16e26af027ff4a919d6c8ee622e2fe642025-08-20T03:02:11ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-03-011210.3389/fvets.2025.15488661548866Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulinYoutian Deng0Xiaoli Wu1Yingying Wan2Junliang Deng3Junliang Deng4Huidan Deng5Huidan Deng6College of Food Science, Sichuan Agriculture University, Yaan, Sichuan, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agriculture University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agriculture University, Chengdu, ChinaIntroductionThe soybean 7S globulin is a major allergen responsible for diarrhea in weaned piglets, leading to significant economic loss in the pig breeding industry. Therefore, there is an urgent need to find effective and safe therapeutic agents to prevent and treat diarrhea caused by soybean 7S globulin. Given the global trend toward “antibiotic alternatives,” traditional Chinese medicines (TCMs) offer a promising approach for the prevention and control of animal diseases.MethodsIn this study, four TCM monomers (curcumin, eleutheroside E, saponin B4, and forsythia A) were evaluated for their protective and therapeutic effects on intestinal epithelial cells (IPEC-J2) damaged by soybean 7S globulin. The CCK8 assay, western blot assay, Elisa assay, and PCR assay were used in this study.ResultsThe results demonstrated that curcumin at concentrations of 0.02, 0.04, and 0.08 μg/mL, eleutheroside E at 25, and 50 μg/mL, saponin B4 at 12.5, 25, and 50 μg/mL, and forsythia A at 20, and 40 μg/mL had significant ameliorative effects on cell viability, permeability, and integrity. Furthermore, the TCM monomers alleviated the inflammatory response, reduced the disruption of tight junctions, and improved the cellular mechanical barrier. These protective effects were likely mediated through the inhibition of the Rho/ROCK signaling pathway, characterized by down regulation of RhoA, ROCK1, ROCK2, and MLKC expression.DiscussionThese findings suggest that the four TCM monomers have the potential to treat diarrhea of weaned piglets caused by soybean protein.https://www.frontiersin.org/articles/10.3389/fvets.2025.1548866/fullsoybean 7S globulincurcumineleutheroside Esaponin B4forsythia Amechanical barrier |
| spellingShingle | Youtian Deng Xiaoli Wu Yingying Wan Junliang Deng Junliang Deng Huidan Deng Huidan Deng Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin Frontiers in Veterinary Science soybean 7S globulin curcumin eleutheroside E saponin B4 forsythia A mechanical barrier |
| title | Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin |
| title_full | Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin |
| title_fullStr | Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin |
| title_full_unstemmed | Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin |
| title_short | Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin |
| title_sort | effect of four traditional chinese medicine monomers on mechanical barrier damage and inflammation response of ipec j2 cells caused by soybean 7s globulin |
| topic | soybean 7S globulin curcumin eleutheroside E saponin B4 forsythia A mechanical barrier |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1548866/full |
| work_keys_str_mv | AT youtiandeng effectoffourtraditionalchinesemedicinemonomersonmechanicalbarrierdamageandinflammationresponseofipecj2cellscausedbysoybean7sglobulin AT xiaoliwu effectoffourtraditionalchinesemedicinemonomersonmechanicalbarrierdamageandinflammationresponseofipecj2cellscausedbysoybean7sglobulin AT yingyingwan effectoffourtraditionalchinesemedicinemonomersonmechanicalbarrierdamageandinflammationresponseofipecj2cellscausedbysoybean7sglobulin AT junliangdeng effectoffourtraditionalchinesemedicinemonomersonmechanicalbarrierdamageandinflammationresponseofipecj2cellscausedbysoybean7sglobulin AT junliangdeng effectoffourtraditionalchinesemedicinemonomersonmechanicalbarrierdamageandinflammationresponseofipecj2cellscausedbysoybean7sglobulin AT huidandeng effectoffourtraditionalchinesemedicinemonomersonmechanicalbarrierdamageandinflammationresponseofipecj2cellscausedbysoybean7sglobulin AT huidandeng effectoffourtraditionalchinesemedicinemonomersonmechanicalbarrierdamageandinflammationresponseofipecj2cellscausedbysoybean7sglobulin |