Genetic diversity of human metapneumovirus (Pneumoviridae: <i>Metapneumovirus</i>) in Russia: results of molecular analysis
Introduction. Human metapneumovirus (hMPV) holds significant epidemiological importance, being a dominant cause of lower respiratory tract infections in children under two years of age and individuals over 65. Multiple infections with hMPV throughout a person’s life are possible due to the antigenic...
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Central Research Institute for Epidemiology
2025-04-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/16718/970 |
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| author | Artem V. Fadeev Yan V. Ivanov Polina A. Petrova Alexander A. Perederiy Maria M. Pisareva Alexey D. Moshkin Andrey B. Komissarov Daria M. Danilenko Dmitriy A. Lioznov |
| author_facet | Artem V. Fadeev Yan V. Ivanov Polina A. Petrova Alexander A. Perederiy Maria M. Pisareva Alexey D. Moshkin Andrey B. Komissarov Daria M. Danilenko Dmitriy A. Lioznov |
| author_sort | Artem V. Fadeev |
| collection | DOAJ |
| description | Introduction. Human metapneumovirus (hMPV) holds significant epidemiological importance, being a dominant cause of lower respiratory tract infections in children under two years of age and individuals over 65. Multiple infections with hMPV throughout a person’s life are possible due to the antigenic and genetic variability of the virus. However, the genetic variability of hMPV circulating in Russia remains unexplored.
Aim of the study. The aim of this study was to test a protocol for whole-genome sequencing of hMPV to assess the genetic diversity of metapneumoviruses circulating in certain regions of Russia.
Materials and methods. Nasopharyngeal swabs were collected from patients of different ages with acute respiratory viral infections (ARVI) tested positive for hMPV using polymerase chain reaction (PCR). From some of the samples, viral isolates were obtained in cell culture. Whole-genome sequencing was performed on both swabs and isolates using the MiSeq Illumina platform, followed by phylogenetic analysis.
Results. For the first time in Russia, whole-genome sequencing of 44 hMPV strains circulating from 2017 to 2024 was conducted. Their genetic group affiliation was described, with the A2b2 clade shown to dominate. It was confirmed that the greatest variability among genes encoding viral surface proteins was observed in the G gene, while changes in the F gene were minimal during the studied period.
Conclusion. The study provides insights into the genetic diversity of hMPV strains circulating in various regions of the Russian Federation. Understanding the genetic variability of hMPV is crucial for comprehending viral evolution, transmission dynamics, and mechanisms of immune evasion, which influence the development of vaccines and antiviral drugs. |
| format | Article |
| id | doaj-art-16dae7a19a17465d92ba4673ba55c4bf |
| institution | DOAJ |
| issn | 0507-4088 2411-2097 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj-art-16dae7a19a17465d92ba4673ba55c4bf2025-08-20T03:14:50ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972025-04-0170216417610.36233/0507-4088-2941272Genetic diversity of human metapneumovirus (Pneumoviridae: <i>Metapneumovirus</i>) in Russia: results of molecular analysisArtem V. Fadeev0https://orcid.org/0000-0003-3558-3261Yan V. Ivanov1https://orcid.org/0009-0006-0187-4000Polina A. Petrova2https://orcid.org/0000-0001-8527-7946Alexander A. Perederiy3https://orcid.org/0000-0002-5961-1856Maria M. Pisareva4https://orcid.org/0000-0002-1499-9957Alexey D. Moshkin5https://orcid.org/0000-0002-1182-8247Andrey B. Komissarov6https://orcid.org/0000-0003-1733-1255Daria M. Danilenko7https://orcid.org/0000-0001-6174-0836Dmitriy A. Lioznov8https://orcid.org/0000-0003-3643-7354A.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian FederationA.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian FederationA.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian FederationA.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian FederationA.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian FederationResearch Institute of Virology, Federal Research Center for Fundamental and Translational MedicineA.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian FederationA.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian FederationA.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian FederationIntroduction. Human metapneumovirus (hMPV) holds significant epidemiological importance, being a dominant cause of lower respiratory tract infections in children under two years of age and individuals over 65. Multiple infections with hMPV throughout a person’s life are possible due to the antigenic and genetic variability of the virus. However, the genetic variability of hMPV circulating in Russia remains unexplored. Aim of the study. The aim of this study was to test a protocol for whole-genome sequencing of hMPV to assess the genetic diversity of metapneumoviruses circulating in certain regions of Russia. Materials and methods. Nasopharyngeal swabs were collected from patients of different ages with acute respiratory viral infections (ARVI) tested positive for hMPV using polymerase chain reaction (PCR). From some of the samples, viral isolates were obtained in cell culture. Whole-genome sequencing was performed on both swabs and isolates using the MiSeq Illumina platform, followed by phylogenetic analysis. Results. For the first time in Russia, whole-genome sequencing of 44 hMPV strains circulating from 2017 to 2024 was conducted. Their genetic group affiliation was described, with the A2b2 clade shown to dominate. It was confirmed that the greatest variability among genes encoding viral surface proteins was observed in the G gene, while changes in the F gene were minimal during the studied period. Conclusion. The study provides insights into the genetic diversity of hMPV strains circulating in various regions of the Russian Federation. Understanding the genetic variability of hMPV is crucial for comprehending viral evolution, transmission dynamics, and mechanisms of immune evasion, which influence the development of vaccines and antiviral drugs.https://virusjour.crie.ru/jour/article/viewFile/16718/970human metapneumoviruswhole-genome sequencinggenetic variabilitygene fgene gduplications |
| spellingShingle | Artem V. Fadeev Yan V. Ivanov Polina A. Petrova Alexander A. Perederiy Maria M. Pisareva Alexey D. Moshkin Andrey B. Komissarov Daria M. Danilenko Dmitriy A. Lioznov Genetic diversity of human metapneumovirus (Pneumoviridae: <i>Metapneumovirus</i>) in Russia: results of molecular analysis Вопросы вирусологии human metapneumovirus whole-genome sequencing genetic variability gene f gene g duplications |
| title | Genetic diversity of human metapneumovirus (Pneumoviridae: <i>Metapneumovirus</i>) in Russia: results of molecular analysis |
| title_full | Genetic diversity of human metapneumovirus (Pneumoviridae: <i>Metapneumovirus</i>) in Russia: results of molecular analysis |
| title_fullStr | Genetic diversity of human metapneumovirus (Pneumoviridae: <i>Metapneumovirus</i>) in Russia: results of molecular analysis |
| title_full_unstemmed | Genetic diversity of human metapneumovirus (Pneumoviridae: <i>Metapneumovirus</i>) in Russia: results of molecular analysis |
| title_short | Genetic diversity of human metapneumovirus (Pneumoviridae: <i>Metapneumovirus</i>) in Russia: results of molecular analysis |
| title_sort | genetic diversity of human metapneumovirus pneumoviridae i metapneumovirus i in russia results of molecular analysis |
| topic | human metapneumovirus whole-genome sequencing genetic variability gene f gene g duplications |
| url | https://virusjour.crie.ru/jour/article/viewFile/16718/970 |
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