Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing
Abstract Atmospheric and Room Temperature Plasma (ARTP) mutagenesis has emerged as a novel and powerful physical mutation technology for microbial strain improvement recently. ARTP operates at atmospheric pressure and room temperature using a helium plasma jet, inducing widespread genomic mutations...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-06-01
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| Series: | Bioresources and Bioprocessing |
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| Online Access: | https://doi.org/10.1186/s40643-025-00907-3 |
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| author | Yu-Hsiu Li Jiun-Jang Juo I-Son Ng |
| author_facet | Yu-Hsiu Li Jiun-Jang Juo I-Son Ng |
| author_sort | Yu-Hsiu Li |
| collection | DOAJ |
| description | Abstract Atmospheric and Room Temperature Plasma (ARTP) mutagenesis has emerged as a novel and powerful physical mutation technology for microbial strain improvement recently. ARTP operates at atmospheric pressure and room temperature using a helium plasma jet, inducing widespread genomic mutations through reactive species and DNA damage. Compared to traditional mutagenesis methods, ARTP is safer, more efficient, and capable of producing high mutation rates without genetic modification, making it a valuable and sophisticated tool in biomanufacturing. This review outlines the principles and diverse applications of ARTP technology for enhancing enzyme activity, metabolite yields, and stress tolerance across various organisms. It also provides a comprehensive discussion of underlying biological mechanisms, workflow, optimization parameters, and potential cellular instability associated with ARTP-induced mutagenesis. Finally, current breakthroughs and future perspectives of ARTP mutagenesis are addressed, emphasizing its role in advancing next-generation microbial platforms for industrial biotechnology and environmental sustainability. Graphical Abstract |
| format | Article |
| id | doaj-art-16ce6a958f5149adbff1a02bc2f2db80 |
| institution | OA Journals |
| issn | 2197-4365 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj-art-16ce6a958f5149adbff1a02bc2f2db802025-08-20T02:10:33ZengSpringerOpenBioresources and Bioprocessing2197-43652025-06-0112111410.1186/s40643-025-00907-3Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturingYu-Hsiu Li0Jiun-Jang Juo1I-Son Ng2Department of Chemical Engineering, National Cheng Kung UniversityDepartment of Chemical Engineering, National Cheng Kung UniversityDepartment of Chemical Engineering, National Cheng Kung UniversityAbstract Atmospheric and Room Temperature Plasma (ARTP) mutagenesis has emerged as a novel and powerful physical mutation technology for microbial strain improvement recently. ARTP operates at atmospheric pressure and room temperature using a helium plasma jet, inducing widespread genomic mutations through reactive species and DNA damage. Compared to traditional mutagenesis methods, ARTP is safer, more efficient, and capable of producing high mutation rates without genetic modification, making it a valuable and sophisticated tool in biomanufacturing. This review outlines the principles and diverse applications of ARTP technology for enhancing enzyme activity, metabolite yields, and stress tolerance across various organisms. It also provides a comprehensive discussion of underlying biological mechanisms, workflow, optimization parameters, and potential cellular instability associated with ARTP-induced mutagenesis. Finally, current breakthroughs and future perspectives of ARTP mutagenesis are addressed, emphasizing its role in advancing next-generation microbial platforms for industrial biotechnology and environmental sustainability. Graphical Abstracthttps://doi.org/10.1186/s40643-025-00907-3BiomanufacturingAtmospheric room temperature plasmaYeastMicroalgaeHigh-value chemical |
| spellingShingle | Yu-Hsiu Li Jiun-Jang Juo I-Son Ng Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing Bioresources and Bioprocessing Biomanufacturing Atmospheric room temperature plasma Yeast Microalgae High-value chemical |
| title | Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing |
| title_full | Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing |
| title_fullStr | Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing |
| title_full_unstemmed | Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing |
| title_short | Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biomanufacturing |
| title_sort | current breakthroughs and advances in atmospheric room temperature plasma artp technology for biomanufacturing |
| topic | Biomanufacturing Atmospheric room temperature plasma Yeast Microalgae High-value chemical |
| url | https://doi.org/10.1186/s40643-025-00907-3 |
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