Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury.
During Clonorchis sinensis (C. sinensis) infection, pro-inflammatory macrophages (M1 macrophages) are highly activated, yet their role in the disease remains poorly understood. Previous studies have demonstrated that extracellular vesicles from C. sinensis (CsEVs) can activate these macrophages, and...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-05-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0013080 |
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| author | Beibei Zhang Xing Li Qian-Yang Zhou Chen Zhang Zheng-Rui Bian Xin-Xin Ren Qian Yu Hui Hua Zhihua Jiang Bo Zhang Xiang-Yang Li Mu-Xin Chen Kui-Yang Zheng Chao Yan |
| author_facet | Beibei Zhang Xing Li Qian-Yang Zhou Chen Zhang Zheng-Rui Bian Xin-Xin Ren Qian Yu Hui Hua Zhihua Jiang Bo Zhang Xiang-Yang Li Mu-Xin Chen Kui-Yang Zheng Chao Yan |
| author_sort | Beibei Zhang |
| collection | DOAJ |
| description | During Clonorchis sinensis (C. sinensis) infection, pro-inflammatory macrophages (M1 macrophages) are highly activated, yet their role in the disease remains poorly understood. Previous studies have demonstrated that extracellular vesicles from C. sinensis (CsEVs) can activate these macrophages, and inhibiting a specific miRNA (Csi-let-7a-5p) in CsEVs (InCsEVs) can reduce this activation. In the present study, liver macrophages in mice were removed using clodronate liposomes (Clodlip). Subsequently, different types of bone marrow-derived macrophages (BMDMs) were adoptively transferred into the mice lacking liver macrophages: untreated (PBS-BMDM), treated with CsEVs (CsEVs-BMDM), treated with a control (ScrCsEVs-BMDM), or treated with InCsEVs (InCsEVs-BMDM). Biliary damages were then evaluated. The results indicated that the transferred macrophages successfully repopulated the mice. CsEVs-BMDM led to significant inflammation and bile duct damage, accompanied by higher levels of inflammatory cytokines (TNF-α and IL-1β). However, when macrophages were treated with InCsEVs, the damage and inflammation were alleviated, and the levels of TNF-α and IL-1β decreased. These findings suggest that pro-inflammatory macrophages activated by CsEVs, especially through Csi-let-7a-5p, play a crucial role in biliary damage during C. sinensis infection. Although other immune cells may also be involved, this study emphasizes the significance of pro-inflammatory macrophages in clonorchiasis. |
| format | Article |
| id | doaj-art-16ce65d01d3d493cb19930bfb8fffd07 |
| institution | Kabale University |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-16ce65d01d3d493cb19930bfb8fffd072025-08-20T03:47:33ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352025-05-01195e001308010.1371/journal.pntd.0013080Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury.Beibei ZhangXing LiQian-Yang ZhouChen ZhangZheng-Rui BianXin-Xin RenQian YuHui HuaZhihua JiangBo ZhangXiang-Yang LiMu-Xin ChenKui-Yang ZhengChao YanDuring Clonorchis sinensis (C. sinensis) infection, pro-inflammatory macrophages (M1 macrophages) are highly activated, yet their role in the disease remains poorly understood. Previous studies have demonstrated that extracellular vesicles from C. sinensis (CsEVs) can activate these macrophages, and inhibiting a specific miRNA (Csi-let-7a-5p) in CsEVs (InCsEVs) can reduce this activation. In the present study, liver macrophages in mice were removed using clodronate liposomes (Clodlip). Subsequently, different types of bone marrow-derived macrophages (BMDMs) were adoptively transferred into the mice lacking liver macrophages: untreated (PBS-BMDM), treated with CsEVs (CsEVs-BMDM), treated with a control (ScrCsEVs-BMDM), or treated with InCsEVs (InCsEVs-BMDM). Biliary damages were then evaluated. The results indicated that the transferred macrophages successfully repopulated the mice. CsEVs-BMDM led to significant inflammation and bile duct damage, accompanied by higher levels of inflammatory cytokines (TNF-α and IL-1β). However, when macrophages were treated with InCsEVs, the damage and inflammation were alleviated, and the levels of TNF-α and IL-1β decreased. These findings suggest that pro-inflammatory macrophages activated by CsEVs, especially through Csi-let-7a-5p, play a crucial role in biliary damage during C. sinensis infection. Although other immune cells may also be involved, this study emphasizes the significance of pro-inflammatory macrophages in clonorchiasis.https://doi.org/10.1371/journal.pntd.0013080 |
| spellingShingle | Beibei Zhang Xing Li Qian-Yang Zhou Chen Zhang Zheng-Rui Bian Xin-Xin Ren Qian Yu Hui Hua Zhihua Jiang Bo Zhang Xiang-Yang Li Mu-Xin Chen Kui-Yang Zheng Chao Yan Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury. PLoS Neglected Tropical Diseases |
| title | Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury. |
| title_full | Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury. |
| title_fullStr | Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury. |
| title_full_unstemmed | Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury. |
| title_short | Clonorchis sinensis extracellular vesicles associated with Csi-let-7a-5p activate pro-inflammatory macrophages to induce biliary injury. |
| title_sort | clonorchis sinensis extracellular vesicles associated with csi let 7a 5p activate pro inflammatory macrophages to induce biliary injury |
| url | https://doi.org/10.1371/journal.pntd.0013080 |
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