CD-Tagging: A New Approach to Gene and Protein Discovery and Analysis
We describe a new method for gene discovery and analysis, CD-tagging, that puts specific molecular tags on a gene, its transcript and its protein product. The method has been successfully tested in two organisms, the haploid unicellular alga Chlamydomonas reinhardtiiand the metazoan Drosophila melan...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
1996-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96205rr03 |
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| author | J.W. Jarvik S.A. Adler C.A. Telmer V. Subramaniam A.J. Lopez |
| author_facet | J.W. Jarvik S.A. Adler C.A. Telmer V. Subramaniam A.J. Lopez |
| author_sort | J.W. Jarvik |
| collection | DOAJ |
| description | We describe a new method for gene discovery and analysis, CD-tagging, that puts specific molecular tags on a gene, its transcript and its protein product. The method has been successfully tested in two organisms, the haploid unicellular alga Chlamydomonas reinhardtiiand the metazoan Drosophila melanogaster. The method utilizes a specially designed DNA molecule, the CDcassette, that contains splice acceptor and donor sites surrounding a short open reading frame. Insertion of the CD-cassette into an intron in a target gene introduces a new exon, represented by the open reading frame of the CD-cassette, surrounded by two functional hybrid introns. As a result (i) the gene is tagged by a specific nucleotide sequence, (ii) the mRNA is tagged by a specific nucleotide sequence and (iii) the protein is tagged by a specific peptide sequence. Because these tags are unique, specific nucleotide or antibody probes can be used to obtain and/or analyze the gene, transcript or protein. As a gene discovery technology, CD-tagging has two unique advantages: 1) Genes can be identified through a primary screen at the protein level, and so the very process by which a gene is identified provides specific empirical information about its biological function. 2) The cassette arms, which are spliced out of the transcript of the target gene, are available to carry a wide variety of DNA sequences, such as genes encoding drug resistance that can be used to select for the presence of the CD-cassette in the genome. |
| format | Article |
| id | doaj-art-16c80ff7f72343538e3fce7c692aeabb |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 1996-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-16c80ff7f72343538e3fce7c692aeabb2025-08-20T02:25:55ZengTaylor & Francis GroupBioTechniques0736-62051940-98181996-05-0120589690410.2144/96205rr03CD-Tagging: A New Approach to Gene and Protein Discovery and AnalysisJ.W. Jarvik0S.A. Adler1C.A. Telmer2V. Subramaniam3A.J. Lopez41Carnegie Mellon University, Pittsburgh, PA1Carnegie Mellon University, Pittsburgh, PA1Carnegie Mellon University, Pittsburgh, PA1Carnegie Mellon University, Pittsburgh, PA1Carnegie Mellon University, Pittsburgh, PAWe describe a new method for gene discovery and analysis, CD-tagging, that puts specific molecular tags on a gene, its transcript and its protein product. The method has been successfully tested in two organisms, the haploid unicellular alga Chlamydomonas reinhardtiiand the metazoan Drosophila melanogaster. The method utilizes a specially designed DNA molecule, the CDcassette, that contains splice acceptor and donor sites surrounding a short open reading frame. Insertion of the CD-cassette into an intron in a target gene introduces a new exon, represented by the open reading frame of the CD-cassette, surrounded by two functional hybrid introns. As a result (i) the gene is tagged by a specific nucleotide sequence, (ii) the mRNA is tagged by a specific nucleotide sequence and (iii) the protein is tagged by a specific peptide sequence. Because these tags are unique, specific nucleotide or antibody probes can be used to obtain and/or analyze the gene, transcript or protein. As a gene discovery technology, CD-tagging has two unique advantages: 1) Genes can be identified through a primary screen at the protein level, and so the very process by which a gene is identified provides specific empirical information about its biological function. 2) The cassette arms, which are spliced out of the transcript of the target gene, are available to carry a wide variety of DNA sequences, such as genes encoding drug resistance that can be used to select for the presence of the CD-cassette in the genome.https://www.future-science.com/doi/10.2144/96205rr03 |
| spellingShingle | J.W. Jarvik S.A. Adler C.A. Telmer V. Subramaniam A.J. Lopez CD-Tagging: A New Approach to Gene and Protein Discovery and Analysis BioTechniques |
| title | CD-Tagging: A New Approach to Gene and Protein Discovery and Analysis |
| title_full | CD-Tagging: A New Approach to Gene and Protein Discovery and Analysis |
| title_fullStr | CD-Tagging: A New Approach to Gene and Protein Discovery and Analysis |
| title_full_unstemmed | CD-Tagging: A New Approach to Gene and Protein Discovery and Analysis |
| title_short | CD-Tagging: A New Approach to Gene and Protein Discovery and Analysis |
| title_sort | cd tagging a new approach to gene and protein discovery and analysis |
| url | https://www.future-science.com/doi/10.2144/96205rr03 |
| work_keys_str_mv | AT jwjarvik cdtagginganewapproachtogeneandproteindiscoveryandanalysis AT saadler cdtagginganewapproachtogeneandproteindiscoveryandanalysis AT catelmer cdtagginganewapproachtogeneandproteindiscoveryandanalysis AT vsubramaniam cdtagginganewapproachtogeneandproteindiscoveryandanalysis AT ajlopez cdtagginganewapproachtogeneandproteindiscoveryandanalysis |