Untargeted Large Volume Hyperthermia Reduces Tumor Drug Uptake From Thermosensitive Liposomes
<italic>Goal:</italic> The impact of hyperthermia (HT) method on tumor drug uptake with thermosensitive liposomes (TSL) is not well understood. <italic>Methods:</italic> We created realistic three-dimensional (3-D) computer models that simulate TSL-encapsulated doxorubicin (T...
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IEEE
2021-01-01
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| Series: | IEEE Open Journal of Engineering in Medicine and Biology |
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| Online Access: | https://ieeexplore.ieee.org/document/9428538/ |
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| author | Krishna Ramajayam A. Wolfe Anjan Motamarry Georges Nahhas John Yost Michael Yost Dieter Haemmerich |
| author_facet | Krishna Ramajayam A. Wolfe Anjan Motamarry Georges Nahhas John Yost Michael Yost Dieter Haemmerich |
| author_sort | Krishna Ramajayam |
| collection | DOAJ |
| description | <italic>Goal:</italic> The impact of hyperthermia (HT) method on tumor drug uptake with thermosensitive liposomes (TSL) is not well understood. <italic>Methods:</italic> We created realistic three-dimensional (3-D) computer models that simulate TSL-encapsulated doxorubicin (TSL-DOX) delivery in mouse tumors with three HT methods (thermistor probe (T), laser (L) and water bath (WB), at 15 min and 60 min HT duration), with corroborating <italic>in vivo</italic> studies. <italic>Results:</italic> Average computer model-predicted tumor drug concentrations (μg/g) were 8.8(T, 15 min), 21.0(T, 60 min), 14.1(L, 15 min), 25.2(L, 60 min), 9.4(WB, 15 min), and 8.7(WB, 60 min). Tumor fluorescence was increased by 2.6 × (T) and 1.6 × (L) when HT duration was extended from 15 to 60 min (p < 0.05), with no increase for WB HT. Pharmacokinetic analysis confirmed that water bath HT causes rapid depletion of encapsulated TSL-DOX in systemic circulation due to the large heated tissue volume. <italic>Conclusions:</italic> Untargeted large volume HT causes poor tumor drug uptake from TSL. |
| format | Article |
| id | doaj-art-16c794f038424a39be8bb48bf8db18c1 |
| institution | Kabale University |
| issn | 2644-1276 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Open Journal of Engineering in Medicine and Biology |
| spelling | doaj-art-16c794f038424a39be8bb48bf8db18c12025-08-20T03:32:40ZengIEEEIEEE Open Journal of Engineering in Medicine and Biology2644-12762021-01-01218719710.1109/OJEMB.2021.30788439428538Untargeted Large Volume Hyperthermia Reduces Tumor Drug Uptake From Thermosensitive LiposomesKrishna Ramajayam0A. Wolfe1Anjan Motamarry2Georges Nahhas3John Yost4Michael Yost5Dieter Haemmerich6https://orcid.org/0000-0003-1127-7024Department of Pediatrics, Medical University of South Carolina, Charleston, SC, USARalph H. Johnson VA Medical Center, Charleston, SC, USADepartment of Pediatrics, Medical University of South Carolina, Charleston, SC, USAHollings Cancer Center, Medical University of South Carolina, Charleston, SC, USADepartment of Surgery, Medical University of South Carolina, Charleston, SC, USADepartment of Surgery, Medical University of South Carolina, Charleston, SC, USADepartment of Pediatrics, Medical University of South Carolina, Charleston, SC, USA<italic>Goal:</italic> The impact of hyperthermia (HT) method on tumor drug uptake with thermosensitive liposomes (TSL) is not well understood. <italic>Methods:</italic> We created realistic three-dimensional (3-D) computer models that simulate TSL-encapsulated doxorubicin (TSL-DOX) delivery in mouse tumors with three HT methods (thermistor probe (T), laser (L) and water bath (WB), at 15 min and 60 min HT duration), with corroborating <italic>in vivo</italic> studies. <italic>Results:</italic> Average computer model-predicted tumor drug concentrations (μg/g) were 8.8(T, 15 min), 21.0(T, 60 min), 14.1(L, 15 min), 25.2(L, 60 min), 9.4(WB, 15 min), and 8.7(WB, 60 min). Tumor fluorescence was increased by 2.6 × (T) and 1.6 × (L) when HT duration was extended from 15 to 60 min (p < 0.05), with no increase for WB HT. Pharmacokinetic analysis confirmed that water bath HT causes rapid depletion of encapsulated TSL-DOX in systemic circulation due to the large heated tissue volume. <italic>Conclusions:</italic> Untargeted large volume HT causes poor tumor drug uptake from TSL.https://ieeexplore.ieee.org/document/9428538/Computer modelsdrug deliveryhyperthermia (HT)<italic xmlns:ali="http://www.niso.org/schemas/ali/1.0/" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">in vivo</italic>thermosensitive liposomes |
| spellingShingle | Krishna Ramajayam A. Wolfe Anjan Motamarry Georges Nahhas John Yost Michael Yost Dieter Haemmerich Untargeted Large Volume Hyperthermia Reduces Tumor Drug Uptake From Thermosensitive Liposomes IEEE Open Journal of Engineering in Medicine and Biology Computer models drug delivery hyperthermia (HT) <italic xmlns:ali="http://www.niso.org/schemas/ali/1.0/" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">in vivo</italic> thermosensitive liposomes |
| title | Untargeted Large Volume Hyperthermia Reduces Tumor Drug Uptake From Thermosensitive Liposomes |
| title_full | Untargeted Large Volume Hyperthermia Reduces Tumor Drug Uptake From Thermosensitive Liposomes |
| title_fullStr | Untargeted Large Volume Hyperthermia Reduces Tumor Drug Uptake From Thermosensitive Liposomes |
| title_full_unstemmed | Untargeted Large Volume Hyperthermia Reduces Tumor Drug Uptake From Thermosensitive Liposomes |
| title_short | Untargeted Large Volume Hyperthermia Reduces Tumor Drug Uptake From Thermosensitive Liposomes |
| title_sort | untargeted large volume hyperthermia reduces tumor drug uptake from thermosensitive liposomes |
| topic | Computer models drug delivery hyperthermia (HT) <italic xmlns:ali="http://www.niso.org/schemas/ali/1.0/" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">in vivo</italic> thermosensitive liposomes |
| url | https://ieeexplore.ieee.org/document/9428538/ |
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