A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech
Leech embryogenesis is a model for investigating cellular and molecular processes of development. Due to the unusually large size of embryonic stem cells (teloblasts: 50–300 μm) in the glossiphoniid leech, Theromyzon tessulatum, and the presence of identifiable stem cell precursors (proteloblasts),...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2010/145183 |
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| author | Kristi A. Hohenstein Shirley A. Lang Tej Nuthulaganti Daniel H. Shain |
| author_facet | Kristi A. Hohenstein Shirley A. Lang Tej Nuthulaganti Daniel H. Shain |
| author_sort | Kristi A. Hohenstein |
| collection | DOAJ |
| description | Leech embryogenesis is a model for investigating cellular and molecular processes of development. Due to the unusually large size of embryonic stem cells (teloblasts: 50–300 μm) in the glossiphoniid leech, Theromyzon tessulatum, and the presence of identifiable stem cell precursors (proteloblasts), we previously isolated a group of genes upregulated upon stem cell birth. In the current study, we show that one of these genes, designated Theromyzon proliferation (Tpr), is required for normal stem cell genesis; specifically, transient Tpr knockdown experiments conducted with antisense oligonucleotides and monitored by semiquantitative RT-PCR, caused abnormal proteloblast proliferation leading to embryonic death, but did not overtly affect neuroectodermal or mesodermal stem cell development once these cells were born. Tpr encodes a large glutamine-rich (∼34%) domain that shares compositional similarity with strong transcriptional enhancers many of which have been linked with trinucleotide repeat disorders (e.g., Huntington's). |
| format | Article |
| id | doaj-art-16c57dcf381d4521b566838d032d04b3 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-16c57dcf381d4521b566838d032d04b32025-08-20T03:54:32ZengWileyStem Cells International1687-96782010-01-01201010.4061/2010/145183145183A Glutamine-Rich Factor Affects Stem Cell Genesis in LeechKristi A. Hohenstein0Shirley A. Lang1Tej Nuthulaganti2Daniel H. Shain3Department of Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102, USADepartment of Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102, USADepartment of Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102, USADepartment of Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102, USALeech embryogenesis is a model for investigating cellular and molecular processes of development. Due to the unusually large size of embryonic stem cells (teloblasts: 50–300 μm) in the glossiphoniid leech, Theromyzon tessulatum, and the presence of identifiable stem cell precursors (proteloblasts), we previously isolated a group of genes upregulated upon stem cell birth. In the current study, we show that one of these genes, designated Theromyzon proliferation (Tpr), is required for normal stem cell genesis; specifically, transient Tpr knockdown experiments conducted with antisense oligonucleotides and monitored by semiquantitative RT-PCR, caused abnormal proteloblast proliferation leading to embryonic death, but did not overtly affect neuroectodermal or mesodermal stem cell development once these cells were born. Tpr encodes a large glutamine-rich (∼34%) domain that shares compositional similarity with strong transcriptional enhancers many of which have been linked with trinucleotide repeat disorders (e.g., Huntington's).http://dx.doi.org/10.4061/2010/145183 |
| spellingShingle | Kristi A. Hohenstein Shirley A. Lang Tej Nuthulaganti Daniel H. Shain A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech Stem Cells International |
| title | A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech |
| title_full | A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech |
| title_fullStr | A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech |
| title_full_unstemmed | A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech |
| title_short | A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech |
| title_sort | glutamine rich factor affects stem cell genesis in leech |
| url | http://dx.doi.org/10.4061/2010/145183 |
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