SFMBT2 regulates plumage color via serum metabolites in Chinese Anyi tile-like gray chickens
ABSTRACT: Plumage color is an important characteristic of chicken breeds, and molecular genetic research is significant for resource conservation and product quality control. Anyi tile-like gray chicken is a high-quality local chicken breed resource generated through long-term natural selection and...
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124009702 |
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| author | Jiguo Xu Qin He Jishang Gong Xuewen Chai Qiao Xu Xinwei Xiong |
| author_facet | Jiguo Xu Qin He Jishang Gong Xuewen Chai Qiao Xu Xinwei Xiong |
| author_sort | Jiguo Xu |
| collection | DOAJ |
| description | ABSTRACT: Plumage color is an important characteristic of chicken breeds, and molecular genetic research is significant for resource conservation and product quality control. Anyi tile-like gray chicken is a high-quality local chicken breed resource generated through long-term natural selection and artificial breeding in China. However, the molecular mechanisms underlying plumage color formation in Anyi tile-like gray chickens remain unclear. In this study, nontargeted liquid chromatography and tandem mass spectrometry (LC–MS/MS) was performed to identify serum metabolites associated with plumage color in 93 Anyi tile-like gray chickens, including 60 tile-like gray and 33 black chickens. Notably, 12 serum metabolites were significantly enriched in Anyi tile-like gray chickens, including deoxyuridine and inosine, which were the key biomarkers distinguishing tile-like gray chickens from black chickens. Additionally, nine serum metabolites were significantly enriched in black chickens. Moreover, we identified 225 significant SNPs (P < 9.71 × 10−8) on chromosomes 1, 2, 3, 4, 11, 15, and 21 that were associated with deoxyuridine, inosine, 3-hydroxybenzoic acid, and L-methionine S-oxide through metabolome genome-wide association studies (mGWAS). Importantly, chromosome 1 harbored a region, 172.79-kb, which was the most likely quantitative trait locus (QTL) interval. RNA sequencing (RNA-seq) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) showed that SFMBT2 was the only differentially expressed gene in the QTL interval, and its expression was correlated with the abundance of specific serum metabolites. Conclusively, SFMBT2-mediated changes in serum metabolites contribute to plumage color development in Anyi tile-like gray chicken. This study provides important insights into the interaction between serum metabolites and host genes, and offers a theoretical basis for the breeding of Anyi tile-like gray chickens. |
| format | Article |
| id | doaj-art-16c4fa7c760c4ff1ae7f89febcc5d776 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-16c4fa7c760c4ff1ae7f89febcc5d7762025-08-20T02:37:48ZengElsevierPoultry Science0032-57912024-12-011031210439110.1016/j.psj.2024.104391SFMBT2 regulates plumage color via serum metabolites in Chinese Anyi tile-like gray chickensJiguo Xu0Qin He1Jishang Gong2Xuewen Chai3Qiao Xu4Xinwei Xiong5Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang, Jiangxi 330032, ChinaJiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang, Jiangxi 330032, ChinaJiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang, Jiangxi 330032, ChinaJiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang, Jiangxi 330032, ChinaJiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang, Jiangxi 330032, ChinaCorresponding author:; Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang, Jiangxi 330032, ChinaABSTRACT: Plumage color is an important characteristic of chicken breeds, and molecular genetic research is significant for resource conservation and product quality control. Anyi tile-like gray chicken is a high-quality local chicken breed resource generated through long-term natural selection and artificial breeding in China. However, the molecular mechanisms underlying plumage color formation in Anyi tile-like gray chickens remain unclear. In this study, nontargeted liquid chromatography and tandem mass spectrometry (LC–MS/MS) was performed to identify serum metabolites associated with plumage color in 93 Anyi tile-like gray chickens, including 60 tile-like gray and 33 black chickens. Notably, 12 serum metabolites were significantly enriched in Anyi tile-like gray chickens, including deoxyuridine and inosine, which were the key biomarkers distinguishing tile-like gray chickens from black chickens. Additionally, nine serum metabolites were significantly enriched in black chickens. Moreover, we identified 225 significant SNPs (P < 9.71 × 10−8) on chromosomes 1, 2, 3, 4, 11, 15, and 21 that were associated with deoxyuridine, inosine, 3-hydroxybenzoic acid, and L-methionine S-oxide through metabolome genome-wide association studies (mGWAS). Importantly, chromosome 1 harbored a region, 172.79-kb, which was the most likely quantitative trait locus (QTL) interval. RNA sequencing (RNA-seq) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) showed that SFMBT2 was the only differentially expressed gene in the QTL interval, and its expression was correlated with the abundance of specific serum metabolites. Conclusively, SFMBT2-mediated changes in serum metabolites contribute to plumage color development in Anyi tile-like gray chicken. This study provides important insights into the interaction between serum metabolites and host genes, and offers a theoretical basis for the breeding of Anyi tile-like gray chickens.http://www.sciencedirect.com/science/article/pii/S0032579124009702plumage colorserum metabolomemGWASRNA-seqAnyi tile-like gray chicken |
| spellingShingle | Jiguo Xu Qin He Jishang Gong Xuewen Chai Qiao Xu Xinwei Xiong SFMBT2 regulates plumage color via serum metabolites in Chinese Anyi tile-like gray chickens Poultry Science plumage color serum metabolome mGWAS RNA-seq Anyi tile-like gray chicken |
| title | SFMBT2 regulates plumage color via serum metabolites in Chinese Anyi tile-like gray chickens |
| title_full | SFMBT2 regulates plumage color via serum metabolites in Chinese Anyi tile-like gray chickens |
| title_fullStr | SFMBT2 regulates plumage color via serum metabolites in Chinese Anyi tile-like gray chickens |
| title_full_unstemmed | SFMBT2 regulates plumage color via serum metabolites in Chinese Anyi tile-like gray chickens |
| title_short | SFMBT2 regulates plumage color via serum metabolites in Chinese Anyi tile-like gray chickens |
| title_sort | sfmbt2 regulates plumage color via serum metabolites in chinese anyi tile like gray chickens |
| topic | plumage color serum metabolome mGWAS RNA-seq Anyi tile-like gray chicken |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124009702 |
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