Effects of Haematococcus pluvialis powder in the diet of golden pompano (Trachinotus ovatus) on the growth performance, pigmentation, hepatic and intestinal health, antioxidant capacity and immunity

Haematococcus pluvialis enriched natural astaxanthin is widely used as a functional additive in the farming industry. The objective of this study was to evaluate the effects of dietary supplementation with H. pluvialis powder on growth performance, pigmentation, hepatic and intestinal morphology, an...

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Main Authors: Jieping Liu, Ziqiao Wang, Yongkang Chen, Yucai Guo, Xiaoming Li, Yanmei Li, Peinan Zhang, Xiangyan Hou, Chun Luo, Wei Zhao, Jin Niu
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513425002613
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author Jieping Liu
Ziqiao Wang
Yongkang Chen
Yucai Guo
Xiaoming Li
Yanmei Li
Peinan Zhang
Xiangyan Hou
Chun Luo
Wei Zhao
Jin Niu
author_facet Jieping Liu
Ziqiao Wang
Yongkang Chen
Yucai Guo
Xiaoming Li
Yanmei Li
Peinan Zhang
Xiangyan Hou
Chun Luo
Wei Zhao
Jin Niu
author_sort Jieping Liu
collection DOAJ
description Haematococcus pluvialis enriched natural astaxanthin is widely used as a functional additive in the farming industry. The objective of this study was to evaluate the effects of dietary supplementation with H. pluvialis powder on growth performance, pigmentation, hepatic and intestinal morphology, antioxidant capacity, and immunity of golden pompano (Trachinotus ovatus). Six isonitrogenous and isolipidic experimental diets, which were formulated to contain 0 % (D1), 0.02 % (D2), 0.04 % (D3), 0.08 % (D4), 0.16 % (D5), and 0.32 % (D6) H. pluvialis powder, were used in the 54-day feeding trial. T. ovatus fed with 0.16 % H. pluvialis showed the greatest levels of FBW, WGR, SGR, and FI. Additionally, across all treatments, the anal fin in the fish given 0.16 % and 0.32 % H. pluvialis displayed increased yellowness (b*), while the tail fin's redness (a*) was considerably higher in the 0.08 % and 0.16 % H. pluvialis treatments. The hepatic morphology result showed that H. pluvialis didn’t influence the health status of the liver, and the activity of CAT decreased in all H. pluvialis-supplemented groups. Compared to other concentrations, the 0.16 % and 0.32 % H. pluvialis groups increased intestinal villi height and amylase activity. The hepatic gene expression showed that 0.16 % H. pluvialis upregulated the expression of kelch ECH-associated protein 1 (keap1), glutathione peroxidase (gsh-px), proliferator-activated receptor α (pparα), interleukin-10 (il-10), and transforming growth factor β (tgf-β), meanwhile, the mRNA level of complement component 4 (c4), superoxide dismutase (sod), and sterol regulatory element-binding protein 1 (srebp1) increased in the intestine. Overall, dietary supplementation with 0.16 % H. pluvialis could improve growth performance, pigmentation in fins, digestive capacity of the intestine, and gene expression related to antioxidant capacity, immunity, and lipid metabolism in T. ovatus.
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spelling doaj-art-16b4eab1fd004b84b8dc57db649826ac2025-08-20T03:40:50ZengElsevierAquaculture Reports2352-51342025-09-014310287510.1016/j.aqrep.2025.102875Effects of Haematococcus pluvialis powder in the diet of golden pompano (Trachinotus ovatus) on the growth performance, pigmentation, hepatic and intestinal health, antioxidant capacity and immunityJieping Liu0Ziqiao Wang1Yongkang Chen2Yucai Guo3Xiaoming Li4Yanmei Li5Peinan Zhang6Xiangyan Hou7Chun Luo8Wei Zhao9Jin Niu10State key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, ChinaState key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, ChinaState key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, ChinaState key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, ChinaState key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, ChinaJ Algae health Science Co., Ltd, USAJ Algae health Science Co., Ltd, USAJ Algae health Science Co., Ltd, USAJ Algae health Science Co., Ltd, USAState key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, China; Corresponding authors.State key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, China; Corresponding authors.Haematococcus pluvialis enriched natural astaxanthin is widely used as a functional additive in the farming industry. The objective of this study was to evaluate the effects of dietary supplementation with H. pluvialis powder on growth performance, pigmentation, hepatic and intestinal morphology, antioxidant capacity, and immunity of golden pompano (Trachinotus ovatus). Six isonitrogenous and isolipidic experimental diets, which were formulated to contain 0 % (D1), 0.02 % (D2), 0.04 % (D3), 0.08 % (D4), 0.16 % (D5), and 0.32 % (D6) H. pluvialis powder, were used in the 54-day feeding trial. T. ovatus fed with 0.16 % H. pluvialis showed the greatest levels of FBW, WGR, SGR, and FI. Additionally, across all treatments, the anal fin in the fish given 0.16 % and 0.32 % H. pluvialis displayed increased yellowness (b*), while the tail fin's redness (a*) was considerably higher in the 0.08 % and 0.16 % H. pluvialis treatments. The hepatic morphology result showed that H. pluvialis didn’t influence the health status of the liver, and the activity of CAT decreased in all H. pluvialis-supplemented groups. Compared to other concentrations, the 0.16 % and 0.32 % H. pluvialis groups increased intestinal villi height and amylase activity. The hepatic gene expression showed that 0.16 % H. pluvialis upregulated the expression of kelch ECH-associated protein 1 (keap1), glutathione peroxidase (gsh-px), proliferator-activated receptor α (pparα), interleukin-10 (il-10), and transforming growth factor β (tgf-β), meanwhile, the mRNA level of complement component 4 (c4), superoxide dismutase (sod), and sterol regulatory element-binding protein 1 (srebp1) increased in the intestine. Overall, dietary supplementation with 0.16 % H. pluvialis could improve growth performance, pigmentation in fins, digestive capacity of the intestine, and gene expression related to antioxidant capacity, immunity, and lipid metabolism in T. ovatus.http://www.sciencedirect.com/science/article/pii/S2352513425002613Haematococcus pluvialisTrachinotus ovatusAstaxanthinPigmentationAntioxidant ability
spellingShingle Jieping Liu
Ziqiao Wang
Yongkang Chen
Yucai Guo
Xiaoming Li
Yanmei Li
Peinan Zhang
Xiangyan Hou
Chun Luo
Wei Zhao
Jin Niu
Effects of Haematococcus pluvialis powder in the diet of golden pompano (Trachinotus ovatus) on the growth performance, pigmentation, hepatic and intestinal health, antioxidant capacity and immunity
Aquaculture Reports
Haematococcus pluvialis
Trachinotus ovatus
Astaxanthin
Pigmentation
Antioxidant ability
title Effects of Haematococcus pluvialis powder in the diet of golden pompano (Trachinotus ovatus) on the growth performance, pigmentation, hepatic and intestinal health, antioxidant capacity and immunity
title_full Effects of Haematococcus pluvialis powder in the diet of golden pompano (Trachinotus ovatus) on the growth performance, pigmentation, hepatic and intestinal health, antioxidant capacity and immunity
title_fullStr Effects of Haematococcus pluvialis powder in the diet of golden pompano (Trachinotus ovatus) on the growth performance, pigmentation, hepatic and intestinal health, antioxidant capacity and immunity
title_full_unstemmed Effects of Haematococcus pluvialis powder in the diet of golden pompano (Trachinotus ovatus) on the growth performance, pigmentation, hepatic and intestinal health, antioxidant capacity and immunity
title_short Effects of Haematococcus pluvialis powder in the diet of golden pompano (Trachinotus ovatus) on the growth performance, pigmentation, hepatic and intestinal health, antioxidant capacity and immunity
title_sort effects of haematococcus pluvialis powder in the diet of golden pompano trachinotus ovatus on the growth performance pigmentation hepatic and intestinal health antioxidant capacity and immunity
topic Haematococcus pluvialis
Trachinotus ovatus
Astaxanthin
Pigmentation
Antioxidant ability
url http://www.sciencedirect.com/science/article/pii/S2352513425002613
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