Bacterial aetiology of chronic otitis media with effusion in children - risk factors
Abstract Background Otitis media with effusion (OME) may occur spontaneously because of poor Eustachian tube function or as an inflammatory response following AOM. Bacterial involvement in OME has been widely reported, with various available methods to identify pathogens from middle ear effusion, in...
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| Language: | English |
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SAGE Publishing
2020-04-01
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| Series: | Journal of Otolaryngology - Head and Neck Surgery |
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| Online Access: | http://link.springer.com/article/10.1186/s40463-020-00418-5 |
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| author | Izabela Korona-Glowniak Agata Wisniewska Marek Juda Karolina Kielbik Grazyna Niedzielska Anna Malm |
| author_facet | Izabela Korona-Glowniak Agata Wisniewska Marek Juda Karolina Kielbik Grazyna Niedzielska Anna Malm |
| author_sort | Izabela Korona-Glowniak |
| collection | DOAJ |
| description | Abstract Background Otitis media with effusion (OME) may occur spontaneously because of poor Eustachian tube function or as an inflammatory response following AOM. Bacterial involvement in OME has been widely reported, with various available methods to identify pathogens from middle ear effusion, including traditional culture methods and polymerase chain reaction (PCR). Objectives The primary goal of this study was to evaluate the bacteriological profile of middle ear effusion in OME. Risk factors of the bacterial OME aetiology were also identified. Methods Middle ear effusions (MEF) from 50 children, aged 2–8 years, diagnosed by ENT and undergoing routine tympanostomy tube placement were collected. MEF samples were streaked on standard microbiological media. Next, DNA was isolated from MEF samples and analysed with multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Alloiococcus otitidis. Results In multiplex PCR assay 37 (74%) of 50 children were positive for at least one of the four microorganisms. In 27.0% positive children multiple bacterial pathogens were identified. A. otitidis was the most frequently identified in positive MEF children (59.5%). By multiplex PCR, H. influenzae, S. pneumoniae and M. catarrhalis were detected in 24, 18 and 8% of OME patients, respectively. There was significant association between bilateral infection and H. influenzae aetiology of OME. Conclusions Overall we found OME predominantly a single otopathogen infection caused mainly by A. otitidis, which is difficult in identification using standard culture method, ahead to S. pneumoniae and H. influenzae. However, one third of MEF samples had multiple bacterial pathogens. |
| format | Article |
| id | doaj-art-16b48bbb720542ea8d83dfd71e320ca7 |
| institution | Kabale University |
| issn | 1916-0216 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Otolaryngology - Head and Neck Surgery |
| spelling | doaj-art-16b48bbb720542ea8d83dfd71e320ca72025-02-03T10:54:12ZengSAGE PublishingJournal of Otolaryngology - Head and Neck Surgery1916-02162020-04-014911710.1186/s40463-020-00418-5Bacterial aetiology of chronic otitis media with effusion in children - risk factorsIzabela Korona-Glowniak0Agata Wisniewska1Marek Juda2Karolina Kielbik3Grazyna Niedzielska4Anna Malm5Department of Pharmaceutical Microbiology, Medical University of LublinDepartment of Pediatric Otolaryngology, Phoniatrics and Audiology, Medical University of LublinDepartment of Pharmaceutical Microbiology, Medical University of LublinDepartment of Pharmaceutical Microbiology, Medical University of LublinDepartment of Pediatric Otolaryngology, Phoniatrics and Audiology, Medical University of LublinDepartment of Pharmaceutical Microbiology, Medical University of LublinAbstract Background Otitis media with effusion (OME) may occur spontaneously because of poor Eustachian tube function or as an inflammatory response following AOM. Bacterial involvement in OME has been widely reported, with various available methods to identify pathogens from middle ear effusion, including traditional culture methods and polymerase chain reaction (PCR). Objectives The primary goal of this study was to evaluate the bacteriological profile of middle ear effusion in OME. Risk factors of the bacterial OME aetiology were also identified. Methods Middle ear effusions (MEF) from 50 children, aged 2–8 years, diagnosed by ENT and undergoing routine tympanostomy tube placement were collected. MEF samples were streaked on standard microbiological media. Next, DNA was isolated from MEF samples and analysed with multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Alloiococcus otitidis. Results In multiplex PCR assay 37 (74%) of 50 children were positive for at least one of the four microorganisms. In 27.0% positive children multiple bacterial pathogens were identified. A. otitidis was the most frequently identified in positive MEF children (59.5%). By multiplex PCR, H. influenzae, S. pneumoniae and M. catarrhalis were detected in 24, 18 and 8% of OME patients, respectively. There was significant association between bilateral infection and H. influenzae aetiology of OME. Conclusions Overall we found OME predominantly a single otopathogen infection caused mainly by A. otitidis, which is difficult in identification using standard culture method, ahead to S. pneumoniae and H. influenzae. However, one third of MEF samples had multiple bacterial pathogens.http://link.springer.com/article/10.1186/s40463-020-00418-5Otitis media with effusionOtopathogensRisk factors |
| spellingShingle | Izabela Korona-Glowniak Agata Wisniewska Marek Juda Karolina Kielbik Grazyna Niedzielska Anna Malm Bacterial aetiology of chronic otitis media with effusion in children - risk factors Journal of Otolaryngology - Head and Neck Surgery Otitis media with effusion Otopathogens Risk factors |
| title | Bacterial aetiology of chronic otitis media with effusion in children - risk factors |
| title_full | Bacterial aetiology of chronic otitis media with effusion in children - risk factors |
| title_fullStr | Bacterial aetiology of chronic otitis media with effusion in children - risk factors |
| title_full_unstemmed | Bacterial aetiology of chronic otitis media with effusion in children - risk factors |
| title_short | Bacterial aetiology of chronic otitis media with effusion in children - risk factors |
| title_sort | bacterial aetiology of chronic otitis media with effusion in children risk factors |
| topic | Otitis media with effusion Otopathogens Risk factors |
| url | http://link.springer.com/article/10.1186/s40463-020-00418-5 |
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