Impact of Combined Acidic and Hyperosmotic Shock Conditions on the Proteome of Listeria monocytogenes ATCC 19115 in a Time-Course Study
Listeria monocytogenes can cause listeriosis in humans through consumption of contaminated food and can adapt to and grow under a wide array of physiochemical stresses. Consequently, it causes persistent food safety issues and requires vigilant sanitation processes to be in place, especially for the...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2019/3075028 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558778374946816 |
|---|---|
| author | Dong Lai Zhang Ya Long Bai John P. Bowman |
| author_facet | Dong Lai Zhang Ya Long Bai John P. Bowman |
| author_sort | Dong Lai Zhang |
| collection | DOAJ |
| description | Listeria monocytogenes can cause listeriosis in humans through consumption of contaminated food and can adapt to and grow under a wide array of physiochemical stresses. Consequently, it causes persistent food safety issues and requires vigilant sanitation processes to be in place, especially for the manufacture of high-risk food products. In this study, the global proteomic responses of the food-borne pathogen L. monocytogenes strain ATCC 19115 were determined when exposed to nonthermal inactivation. This process was examined in the early stationary growth phase with the strain placed under simultaneous exposure to low pH (pH 3.5) and high salinity (aw 0.900, 14% NaCl). Proteomic responses, measured using iTRAQ techniques, were conducted over a time course (5 min, 30 min, and 1 h at 25°C). The enumeration results showed that, at 5 min, cells underwent initial rapid inactivation by 1.2 log units and 2.5 log units after 30 min, and after that, culturability remained stable when sampled at 1 h. From the iTRAQ results, the proteome level changes that occur rapidly during the inactivation process mainly affected prophage, cell defense/detoxification, carbohydrate-related metabolism, transporter proteins, phosphotransferase systems, cell wall biogenesis, and specific cell surface proteins. Pathway map analysis revealed that several pathways are affected including pentose and glucuronate interconversions, glycolysis/gluconeogenesis, pyruvate metabolism, valine, leucine and isoleucine biosynthesis, oxidative phosphorylation, and proteins associated with bacterial invasion of epithelial cells and host survival. Proteome profiling provided a better understanding of the physiological responses of this pathogen to adapt to lethal nonthermal environments and indicates the need to improve food processing and storage methods, especially for non- or minimally thermally processed foods. |
| format | Article |
| id | doaj-art-16b08f22295b48e5916b0e48ef43b3f7 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-16b08f22295b48e5916b0e48ef43b3f72025-02-03T01:31:33ZengWileyJournal of Food Quality0146-94281745-45572019-01-01201910.1155/2019/30750283075028Impact of Combined Acidic and Hyperosmotic Shock Conditions on the Proteome of Listeria monocytogenes ATCC 19115 in a Time-Course StudyDong Lai Zhang0Ya Long Bai1John P. Bowman2Institute of Agri-Food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai, ChinaInstitute of Agri-Food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai, ChinaFood Safety Centre, Tasmanian Institute of Agriculture, University of Tasmania, Hobart, Tasmania, AustraliaListeria monocytogenes can cause listeriosis in humans through consumption of contaminated food and can adapt to and grow under a wide array of physiochemical stresses. Consequently, it causes persistent food safety issues and requires vigilant sanitation processes to be in place, especially for the manufacture of high-risk food products. In this study, the global proteomic responses of the food-borne pathogen L. monocytogenes strain ATCC 19115 were determined when exposed to nonthermal inactivation. This process was examined in the early stationary growth phase with the strain placed under simultaneous exposure to low pH (pH 3.5) and high salinity (aw 0.900, 14% NaCl). Proteomic responses, measured using iTRAQ techniques, were conducted over a time course (5 min, 30 min, and 1 h at 25°C). The enumeration results showed that, at 5 min, cells underwent initial rapid inactivation by 1.2 log units and 2.5 log units after 30 min, and after that, culturability remained stable when sampled at 1 h. From the iTRAQ results, the proteome level changes that occur rapidly during the inactivation process mainly affected prophage, cell defense/detoxification, carbohydrate-related metabolism, transporter proteins, phosphotransferase systems, cell wall biogenesis, and specific cell surface proteins. Pathway map analysis revealed that several pathways are affected including pentose and glucuronate interconversions, glycolysis/gluconeogenesis, pyruvate metabolism, valine, leucine and isoleucine biosynthesis, oxidative phosphorylation, and proteins associated with bacterial invasion of epithelial cells and host survival. Proteome profiling provided a better understanding of the physiological responses of this pathogen to adapt to lethal nonthermal environments and indicates the need to improve food processing and storage methods, especially for non- or minimally thermally processed foods.http://dx.doi.org/10.1155/2019/3075028 |
| spellingShingle | Dong Lai Zhang Ya Long Bai John P. Bowman Impact of Combined Acidic and Hyperosmotic Shock Conditions on the Proteome of Listeria monocytogenes ATCC 19115 in a Time-Course Study Journal of Food Quality |
| title | Impact of Combined Acidic and Hyperosmotic Shock Conditions on the Proteome of Listeria monocytogenes ATCC 19115 in a Time-Course Study |
| title_full | Impact of Combined Acidic and Hyperosmotic Shock Conditions on the Proteome of Listeria monocytogenes ATCC 19115 in a Time-Course Study |
| title_fullStr | Impact of Combined Acidic and Hyperosmotic Shock Conditions on the Proteome of Listeria monocytogenes ATCC 19115 in a Time-Course Study |
| title_full_unstemmed | Impact of Combined Acidic and Hyperosmotic Shock Conditions on the Proteome of Listeria monocytogenes ATCC 19115 in a Time-Course Study |
| title_short | Impact of Combined Acidic and Hyperosmotic Shock Conditions on the Proteome of Listeria monocytogenes ATCC 19115 in a Time-Course Study |
| title_sort | impact of combined acidic and hyperosmotic shock conditions on the proteome of listeria monocytogenes atcc 19115 in a time course study |
| url | http://dx.doi.org/10.1155/2019/3075028 |
| work_keys_str_mv | AT donglaizhang impactofcombinedacidicandhyperosmoticshockconditionsontheproteomeoflisteriamonocytogenesatcc19115inatimecoursestudy AT yalongbai impactofcombinedacidicandhyperosmoticshockconditionsontheproteomeoflisteriamonocytogenesatcc19115inatimecoursestudy AT johnpbowman impactofcombinedacidicandhyperosmoticshockconditionsontheproteomeoflisteriamonocytogenesatcc19115inatimecoursestudy |