New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal
Two Simple, accurate, precise, and rapid spectrophotometric and conductometric methods were developed for the estimation of erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) in both pure and pharmaceutical dosage forms. The spectrophotometric procedure depends on th...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2013/214270 |
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| author | Rania A. Sayed Wafaa S. Hassan Magda Y. El-Mammli Abadalla Shalaby |
| author_facet | Rania A. Sayed Wafaa S. Hassan Magda Y. El-Mammli Abadalla Shalaby |
| author_sort | Rania A. Sayed |
| collection | DOAJ |
| description | Two Simple, accurate, precise, and rapid spectrophotometric and conductometric methods were developed for the estimation of erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) in both pure and pharmaceutical dosage forms. The spectrophotometric procedure depends on the reaction of rose bengal and copper with the cited drugs to form stable ternary complexes which are extractable with methylene chloride, and the absorbances were measured at 558, 557, and 560 nm for (I), (II), and (III), respectively. The conductometric method depends on the formation of an ion-pair complex between the studied drug and rose bengal. For the spectrophotometric method, Beer's law was obeyed. The correlation coefficient () for the studied drugs was found to be 0.9999. The molar absorptivity (), Sandell’s sensitivity, limit of detection (LOD), and limit of quantification (LOQ) were also calculated. The proposed methods were successfully applied for the determination of certain pharmaceutical dosage forms containing the studied drugs |
| format | Article |
| id | doaj-art-16afe42a17be472d8db1b3fa14546dbb |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-16afe42a17be472d8db1b3fa14546dbb2025-02-03T01:21:37ZengWileyJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/214270214270New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose BengalRania A. Sayed0Wafaa S. Hassan1Magda Y. El-Mammli2Abadalla Shalaby3Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptTwo Simple, accurate, precise, and rapid spectrophotometric and conductometric methods were developed for the estimation of erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) in both pure and pharmaceutical dosage forms. The spectrophotometric procedure depends on the reaction of rose bengal and copper with the cited drugs to form stable ternary complexes which are extractable with methylene chloride, and the absorbances were measured at 558, 557, and 560 nm for (I), (II), and (III), respectively. The conductometric method depends on the formation of an ion-pair complex between the studied drug and rose bengal. For the spectrophotometric method, Beer's law was obeyed. The correlation coefficient () for the studied drugs was found to be 0.9999. The molar absorptivity (), Sandell’s sensitivity, limit of detection (LOD), and limit of quantification (LOQ) were also calculated. The proposed methods were successfully applied for the determination of certain pharmaceutical dosage forms containing the studied drugshttp://dx.doi.org/10.1155/2013/214270 |
| spellingShingle | Rania A. Sayed Wafaa S. Hassan Magda Y. El-Mammli Abadalla Shalaby New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal Journal of Spectroscopy |
| title | New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal |
| title_full | New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal |
| title_fullStr | New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal |
| title_full_unstemmed | New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal |
| title_short | New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal |
| title_sort | new spectrophotometric and conductometric methods for macrolide antibiotics determination in pure and pharmaceutical dosage forms using rose bengal |
| url | http://dx.doi.org/10.1155/2013/214270 |
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