Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistance
The objective of the study: to identify the frequency of various species of non-tuberculous mycobacteria (NTM) isolated in the patients of TB services in Siberian Region during 2014-2018, to determine the correlation between rates of resistance to different antibacterial drugs.Subjects and methods. ...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
New Terra Publishing House
2019-11-01
|
| Series: | Туберкулез и болезни лёгких |
| Subjects: | |
| Online Access: | https://www.tibl-journal.com/jour/article/view/1339 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850282837948760064 |
|---|---|
| author | O. I. Аlkhovik I. O. Meshkov T. I. Petrenko L. N. Evdokimova |
| author_facet | O. I. Аlkhovik I. O. Meshkov T. I. Petrenko L. N. Evdokimova |
| author_sort | O. I. Аlkhovik |
| collection | DOAJ |
| description | The objective of the study: to identify the frequency of various species of non-tuberculous mycobacteria (NTM) isolated in the patients of TB services in Siberian Region during 2014-2018, to determine the correlation between rates of resistance to different antibacterial drugs.Subjects and methods. PCR hybridization on strip strips (Hain Lifescience GenoType Mycobacterium CM / AS) was used to genotype 475 NTM isolates of patients from various TB hospitals inSiberia. Drug susceptibility was tested by minimum inhibitory concentrations (MIC) on culture with serial micro-dilutions in a liquid medium using TREK Diagnostic Systems kits. 64 panels were used for slow-growing mycobacteria (SLOWMYCO), 46 - for fast-growing ones(RAPMYCO). Data processing was performed using the APL of 3.5.1 and the data analysis software of Rstudio, version 1.1.442.Results of the study1. Tomsk Region differs from the other considered regions ofSiberiadue to higher frequency of M. intracellulare among isolates identified by PCR hybridization. In other regions, M. fortuitum prevails among isolated.2. Incomparison with the reference data (CLSI M62, 2018), differences in the MIC of some NTM species isolated from patients in Siberia and theFar Eastwere observed, most likely related to the regional characteristics of these isolates.3. HTM isolates resistant to moxifloxacin are also resistant to linezolid, trimethoprim, and ciprofloxacin.4. Fast-growing clarithromycin resistant HTM isolates are predominantly sensitive to moxifloxacin, ciprofloxacin, and trimethoprim. |
| format | Article |
| id | doaj-art-16aeb9bffe464eb98c6b658218d6d312 |
| institution | OA Journals |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| publishDate | 2019-11-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj-art-16aeb9bffe464eb98c6b658218d6d3122025-08-20T01:47:52ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062019-11-01971051110.21292/2075-1230-2019-97-10-5-111339Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistanceO. I. Аlkhovik0I. O. Meshkov1T. I. Petrenko2L. N. Evdokimova3Novosibirsk Tuberculosis Research InstituteNovosibirsk Tuberculosis Research InstituteNovosibirsk Tuberculosis Research Institute; Novosibirsk State Medical UniversityNovosibirsk Tuberculosis Research InstituteThe objective of the study: to identify the frequency of various species of non-tuberculous mycobacteria (NTM) isolated in the patients of TB services in Siberian Region during 2014-2018, to determine the correlation between rates of resistance to different antibacterial drugs.Subjects and methods. PCR hybridization on strip strips (Hain Lifescience GenoType Mycobacterium CM / AS) was used to genotype 475 NTM isolates of patients from various TB hospitals inSiberia. Drug susceptibility was tested by minimum inhibitory concentrations (MIC) on culture with serial micro-dilutions in a liquid medium using TREK Diagnostic Systems kits. 64 panels were used for slow-growing mycobacteria (SLOWMYCO), 46 - for fast-growing ones(RAPMYCO). Data processing was performed using the APL of 3.5.1 and the data analysis software of Rstudio, version 1.1.442.Results of the study1. Tomsk Region differs from the other considered regions ofSiberiadue to higher frequency of M. intracellulare among isolates identified by PCR hybridization. In other regions, M. fortuitum prevails among isolated.2. Incomparison with the reference data (CLSI M62, 2018), differences in the MIC of some NTM species isolated from patients in Siberia and theFar Eastwere observed, most likely related to the regional characteristics of these isolates.3. HTM isolates resistant to moxifloxacin are also resistant to linezolid, trimethoprim, and ciprofloxacin.4. Fast-growing clarithromycin resistant HTM isolates are predominantly sensitive to moxifloxacin, ciprofloxacin, and trimethoprim.https://www.tibl-journal.com/jour/article/view/1339non-tuberculous mycobacteriaisolatemycobacteriosisdrug resistancediagnostics |
| spellingShingle | O. I. Аlkhovik I. O. Meshkov T. I. Petrenko L. N. Evdokimova Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistance Туберкулез и болезни лёгких non-tuberculous mycobacteria isolate mycobacteriosis drug resistance diagnostics |
| title | Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistance |
| title_full | Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistance |
| title_fullStr | Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistance |
| title_full_unstemmed | Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistance |
| title_short | Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistance |
| title_sort | identification of non tuberculous mycobacteria circulating in different regions of siberia and analysis of their drug resistance |
| topic | non-tuberculous mycobacteria isolate mycobacteriosis drug resistance diagnostics |
| url | https://www.tibl-journal.com/jour/article/view/1339 |
| work_keys_str_mv | AT oialkhovik identificationofnontuberculousmycobacteriacirculatingindifferentregionsofsiberiaandanalysisoftheirdrugresistance AT iomeshkov identificationofnontuberculousmycobacteriacirculatingindifferentregionsofsiberiaandanalysisoftheirdrugresistance AT tipetrenko identificationofnontuberculousmycobacteriacirculatingindifferentregionsofsiberiaandanalysisoftheirdrugresistance AT lnevdokimova identificationofnontuberculousmycobacteriacirculatingindifferentregionsofsiberiaandanalysisoftheirdrugresistance |