Genome-wide identification of the cytochrome P450 superfamily in Trichoplusia ni and in-silico expression of resistance against HDAC inhibitors
Abstract The cabbage looper, Trichoplusia ni, is a serious pest of cruciferous crops such as cabbage, cauliflower, collard/spring greens, and horseradish, with a major financial impact. Despite its significant financial importance, there has been little study on the characterization of Cytochrome P4...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-16919-0 |
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| author | Arsalan Ahmad Adnan Sami Muhammad Zeshan Haider Muhammad Shafiq Shahbaz Ahmad Adnan Iqbal Qurban Ali Muhammad Arshad Javed Muhammad Ashfaq Shiming Han Daoud Ali |
| author_facet | Arsalan Ahmad Adnan Sami Muhammad Zeshan Haider Muhammad Shafiq Shahbaz Ahmad Adnan Iqbal Qurban Ali Muhammad Arshad Javed Muhammad Ashfaq Shiming Han Daoud Ali |
| author_sort | Arsalan Ahmad |
| collection | DOAJ |
| description | Abstract The cabbage looper, Trichoplusia ni, is a serious pest of cruciferous crops such as cabbage, cauliflower, collard/spring greens, and horseradish, with a major financial impact. Despite its significant financial importance, there has been little study on the characterization of Cytochrome P450 (CYP450) genes, which are critical components in the control of metabolic activities. This study discovers and investigates 19 CYP450 genes in T. ni that are involved in important metabolic processes such as fatty acid metabolism, resistance mechanisms, phytotoxin response, and insecticide detoxification. We identified important CYP450 genes that play critical roles in conferring resistance to HDAC inhibitor insecticides, particularly sulforaphane and Trichostatin A, using in silico gene expression profiling. The findings reveal insight into the importance of identified genes in detoxifying chemical treatments, which may contribute to the advancement of resistance in cabbage looper populations. Notably, CYP4C1-1, CYP4C1-like-1, and CYP4C1-2emerge as key genes, significantly contributing to resistance and detoxification processes under the effect of HDAC inhibitor-induced chemical stressors. This study provides insight on the genetic mechanisms behind resistance in T. ni, providing important information on potential targets for pest control. These initiatives will pave the way for the development of innovative and effective control measures for cabbage looper populations in agricultural areas. |
| format | Article |
| id | doaj-art-169f2be57a1f4cfaaa2c51af40c256e6 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-169f2be57a1f4cfaaa2c51af40c256e62025-08-24T11:19:33ZengNature PortfolioScientific Reports2045-23222025-08-0115111110.1038/s41598-025-16919-0Genome-wide identification of the cytochrome P450 superfamily in Trichoplusia ni and in-silico expression of resistance against HDAC inhibitorsArsalan Ahmad0Adnan Sami1Muhammad Zeshan Haider2Muhammad Shafiq3Shahbaz Ahmad4Adnan Iqbal5Qurban Ali6Muhammad Arshad Javed7Muhammad Ashfaq8Shiming Han9Daoud Ali10Department of Entomology, Faculty of Agricultural Sciences, University of the PunjabGraduate Institute of Biotechnology, National Cheng Hsing UniversityGraduate Institute of Biotechnology, National Cheng Hsing UniversityDepartment of Horticulture, Faculty of Agriculture Sciences, University of the PunjabDepartment of Entomology, Faculty of Agricultural Sciences, University of the PunjabPlant Breeding and Acclimatization Institute—National Research InstituteDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the PunjabDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the PunjabDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the PunjabSchool of Biological Sciences and Technology, Liupanshui Normal UniversityDepartment of Zoology, College of Science, King Saud UniversityAbstract The cabbage looper, Trichoplusia ni, is a serious pest of cruciferous crops such as cabbage, cauliflower, collard/spring greens, and horseradish, with a major financial impact. Despite its significant financial importance, there has been little study on the characterization of Cytochrome P450 (CYP450) genes, which are critical components in the control of metabolic activities. This study discovers and investigates 19 CYP450 genes in T. ni that are involved in important metabolic processes such as fatty acid metabolism, resistance mechanisms, phytotoxin response, and insecticide detoxification. We identified important CYP450 genes that play critical roles in conferring resistance to HDAC inhibitor insecticides, particularly sulforaphane and Trichostatin A, using in silico gene expression profiling. The findings reveal insight into the importance of identified genes in detoxifying chemical treatments, which may contribute to the advancement of resistance in cabbage looper populations. Notably, CYP4C1-1, CYP4C1-like-1, and CYP4C1-2emerge as key genes, significantly contributing to resistance and detoxification processes under the effect of HDAC inhibitor-induced chemical stressors. This study provides insight on the genetic mechanisms behind resistance in T. ni, providing important information on potential targets for pest control. These initiatives will pave the way for the development of innovative and effective control measures for cabbage looper populations in agricultural areas.https://doi.org/10.1038/s41598-025-16919-0Genome-wideTrichoplusia niCabbage looperInsecticide resistanceHDAC inhibitorsSulforaphane |
| spellingShingle | Arsalan Ahmad Adnan Sami Muhammad Zeshan Haider Muhammad Shafiq Shahbaz Ahmad Adnan Iqbal Qurban Ali Muhammad Arshad Javed Muhammad Ashfaq Shiming Han Daoud Ali Genome-wide identification of the cytochrome P450 superfamily in Trichoplusia ni and in-silico expression of resistance against HDAC inhibitors Scientific Reports Genome-wide Trichoplusia ni Cabbage looper Insecticide resistance HDAC inhibitors Sulforaphane |
| title | Genome-wide identification of the cytochrome P450 superfamily in Trichoplusia ni and in-silico expression of resistance against HDAC inhibitors |
| title_full | Genome-wide identification of the cytochrome P450 superfamily in Trichoplusia ni and in-silico expression of resistance against HDAC inhibitors |
| title_fullStr | Genome-wide identification of the cytochrome P450 superfamily in Trichoplusia ni and in-silico expression of resistance against HDAC inhibitors |
| title_full_unstemmed | Genome-wide identification of the cytochrome P450 superfamily in Trichoplusia ni and in-silico expression of resistance against HDAC inhibitors |
| title_short | Genome-wide identification of the cytochrome P450 superfamily in Trichoplusia ni and in-silico expression of resistance against HDAC inhibitors |
| title_sort | genome wide identification of the cytochrome p450 superfamily in trichoplusia ni and in silico expression of resistance against hdac inhibitors |
| topic | Genome-wide Trichoplusia ni Cabbage looper Insecticide resistance HDAC inhibitors Sulforaphane |
| url | https://doi.org/10.1038/s41598-025-16919-0 |
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