Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication.
Replication of arboviruses in their arthropod vectors is controlled by innate immune responses. The RNA sequence-specific break down mechanism, RNA interference (RNAi), has been shown to be an important innate antiviral response in mosquitoes. In addition, immune signaling pathways have been reporte...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-07-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0002994 |
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| author | Melanie McFarlane Camilo Arias-Goeta Estelle Martin Zoe O'Hara Aleksei Lulla Laurence Mousson Stephanie M Rainey Suzana Misbah Esther Schnettler Claire L Donald Andres Merits Alain Kohl Anna-Bella Failloux |
| author_facet | Melanie McFarlane Camilo Arias-Goeta Estelle Martin Zoe O'Hara Aleksei Lulla Laurence Mousson Stephanie M Rainey Suzana Misbah Esther Schnettler Claire L Donald Andres Merits Alain Kohl Anna-Bella Failloux |
| author_sort | Melanie McFarlane |
| collection | DOAJ |
| description | Replication of arboviruses in their arthropod vectors is controlled by innate immune responses. The RNA sequence-specific break down mechanism, RNA interference (RNAi), has been shown to be an important innate antiviral response in mosquitoes. In addition, immune signaling pathways have been reported to mediate arbovirus infections in mosquitoes; namely the JAK/STAT, immune deficiency (IMD) and Toll pathways. Very little is known about these pathways in response to chikungunya virus (CHIKV) infection, a mosquito-borne alphavirus (Togaviridae) transmitted by aedine species to humans resulting in a febrile and arthralgic disease. In this study, the contribution of several innate immune responses to control CHIKV replication was investigated. In vitro experiments identified the RNAi pathway as a key antiviral pathway. CHIKV was shown to repress the activity of the Toll signaling pathway in vitro but neither JAK/STAT, IMD nor Toll pathways were found to mediate antiviral activities. In vivo data further confirmed our in vitro identification of the vital role of RNAi in antiviral defence. Taken together these results indicate a complex interaction between CHIKV replication and mosquito innate immune responses and demonstrate similarities as well as differences in the control of alphaviruses and other arboviruses by mosquito immune pathways. |
| format | Article |
| id | doaj-art-1696c58b8e6b4c488f3a6887dae6d69f |
| institution | OA Journals |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2014-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-1696c58b8e6b4c488f3a6887dae6d69f2025-08-20T02:35:36ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352014-07-0187e299410.1371/journal.pntd.0002994Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication.Melanie McFarlaneCamilo Arias-GoetaEstelle MartinZoe O'HaraAleksei LullaLaurence MoussonStephanie M RaineySuzana MisbahEsther SchnettlerClaire L DonaldAndres MeritsAlain KohlAnna-Bella FaillouxReplication of arboviruses in their arthropod vectors is controlled by innate immune responses. The RNA sequence-specific break down mechanism, RNA interference (RNAi), has been shown to be an important innate antiviral response in mosquitoes. In addition, immune signaling pathways have been reported to mediate arbovirus infections in mosquitoes; namely the JAK/STAT, immune deficiency (IMD) and Toll pathways. Very little is known about these pathways in response to chikungunya virus (CHIKV) infection, a mosquito-borne alphavirus (Togaviridae) transmitted by aedine species to humans resulting in a febrile and arthralgic disease. In this study, the contribution of several innate immune responses to control CHIKV replication was investigated. In vitro experiments identified the RNAi pathway as a key antiviral pathway. CHIKV was shown to repress the activity of the Toll signaling pathway in vitro but neither JAK/STAT, IMD nor Toll pathways were found to mediate antiviral activities. In vivo data further confirmed our in vitro identification of the vital role of RNAi in antiviral defence. Taken together these results indicate a complex interaction between CHIKV replication and mosquito innate immune responses and demonstrate similarities as well as differences in the control of alphaviruses and other arboviruses by mosquito immune pathways.https://doi.org/10.1371/journal.pntd.0002994 |
| spellingShingle | Melanie McFarlane Camilo Arias-Goeta Estelle Martin Zoe O'Hara Aleksei Lulla Laurence Mousson Stephanie M Rainey Suzana Misbah Esther Schnettler Claire L Donald Andres Merits Alain Kohl Anna-Bella Failloux Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. PLoS Neglected Tropical Diseases |
| title | Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. |
| title_full | Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. |
| title_fullStr | Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. |
| title_full_unstemmed | Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. |
| title_short | Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. |
| title_sort | characterization of aedes aegypti innate immune pathways that limit chikungunya virus replication |
| url | https://doi.org/10.1371/journal.pntd.0002994 |
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