Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women
Introduction: Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2016-09-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/7161 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850187422482038784 |
|---|---|
| author | Daniele Maria Knupp de Souza Claudio Galuppo Diniz Didier Silveira Castellano Filho Laura Maria Andrade de Oliveira Débora Martins Coelho Luciana de Souza Talha Thiago César do Nascimento Alessandra Barbosa Ferreira-Machado Vânia Lúcia da Silva |
| author_facet | Daniele Maria Knupp de Souza Claudio Galuppo Diniz Didier Silveira Castellano Filho Laura Maria Andrade de Oliveira Débora Martins Coelho Luciana de Souza Talha Thiago César do Nascimento Alessandra Barbosa Ferreira-Machado Vânia Lúcia da Silva |
| author_sort | Daniele Maria Knupp de Souza |
| collection | DOAJ |
| description | Introduction: Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated from BV and non-BV patients.
Methodology: The vaginal secretions were collected randomly and processed for G. vaginalis isolation. The isolates were presumptively identified by β-hemolysis and oxidase and catalase tests. Polymerase chain reaction (PCR) was performed to confirm bacterial identity and to detect the vly gene. Antimicrobial susceptibility patterns were determined.
Results: Of 89 patients, G. vaginalis was isolated from 42 (37 BV and 5 non-BV), and 204 isolates were selected (179 from BV and 25 non-BV). The vly gene was detected in all G. vaginalis isolated from non-BV women and in 98.3% of the bacteria from BV patients. High resistance was observed for ampicillin (54.4%), metronidazole (59.8%), tinidazole (60.3%) and secnidazole (71.6%).
Conclusions: Further studies are needed to better address the role of G. vaginalis and the vly gene in BV pathogenesis.
|
| format | Article |
| id | doaj-art-169651dd4c15425bbd82cbb9f4b18c68 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-169651dd4c15425bbd82cbb9f4b18c682025-08-20T02:16:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-09-01100910.3855/jidc.7161Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed womenDaniele Maria Knupp de Souza0Claudio Galuppo Diniz1Didier Silveira Castellano Filho2Laura Maria Andrade de Oliveira3Débora Martins Coelho4Luciana de Souza Talha5Thiago César do Nascimento6Alessandra Barbosa Ferreira-Machado7Vânia Lúcia da Silva8Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilFederal University of Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilFaculdade de Ciências Médicas e da Saúde de Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilFederal University of Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilFederal University of Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilFederal University of Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilFederal University of Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilFederal University of Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilFederal University of Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilIntroduction: Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated from BV and non-BV patients. Methodology: The vaginal secretions were collected randomly and processed for G. vaginalis isolation. The isolates were presumptively identified by β-hemolysis and oxidase and catalase tests. Polymerase chain reaction (PCR) was performed to confirm bacterial identity and to detect the vly gene. Antimicrobial susceptibility patterns were determined. Results: Of 89 patients, G. vaginalis was isolated from 42 (37 BV and 5 non-BV), and 204 isolates were selected (179 from BV and 25 non-BV). The vly gene was detected in all G. vaginalis isolated from non-BV women and in 98.3% of the bacteria from BV patients. High resistance was observed for ampicillin (54.4%), metronidazole (59.8%), tinidazole (60.3%) and secnidazole (71.6%). Conclusions: Further studies are needed to better address the role of G. vaginalis and the vly gene in BV pathogenesis. https://jidc.org/index.php/journal/article/view/7161bacterial vaginosisGardnerella vaginalisNugent scoreantimicrobial resistanceclue-cells |
| spellingShingle | Daniele Maria Knupp de Souza Claudio Galuppo Diniz Didier Silveira Castellano Filho Laura Maria Andrade de Oliveira Débora Martins Coelho Luciana de Souza Talha Thiago César do Nascimento Alessandra Barbosa Ferreira-Machado Vânia Lúcia da Silva Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women Journal of Infection in Developing Countries bacterial vaginosis Gardnerella vaginalis Nugent score antimicrobial resistance clue-cells |
| title | Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women |
| title_full | Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women |
| title_fullStr | Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women |
| title_full_unstemmed | Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women |
| title_short | Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women |
| title_sort | antimicrobial susceptibility and vaginolysin in gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women |
| topic | bacterial vaginosis Gardnerella vaginalis Nugent score antimicrobial resistance clue-cells |
| url | https://jidc.org/index.php/journal/article/view/7161 |
| work_keys_str_mv | AT danielemariaknuppdesouza antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen AT claudiogaluppodiniz antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen AT didiersilveiracastellanofilho antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen AT lauramariaandradedeoliveira antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen AT deboramartinscoelho antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen AT lucianadesouzatalha antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen AT thiagocesardonascimento antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen AT alessandrabarbosaferreiramachado antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen AT vanialuciadasilva antimicrobialsusceptibilityandvaginolysiningardnerellavaginalisfromhealthyandbacterialvaginosisdiagnosedwomen |