Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells
Calcium phosphate cement (CPC) that is based on α-tricalcium phosphate (α-TCP) is considered desirable for bone tissue engineering because of its relatively rapid degradation properties. However, such cement is relatively weak, restricting its use to areas of low mechanical stress. Wollastonite fibe...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2017/5260106 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850234479927361536 |
|---|---|
| author | Juliana Almeida Domingues Mariana Motisuke Celso Aparecido Bertran Moema A. Hausen Eliana Aparecida de Rezende Duek José Angelo Camilli |
| author_facet | Juliana Almeida Domingues Mariana Motisuke Celso Aparecido Bertran Moema A. Hausen Eliana Aparecida de Rezende Duek José Angelo Camilli |
| author_sort | Juliana Almeida Domingues |
| collection | DOAJ |
| description | Calcium phosphate cement (CPC) that is based on α-tricalcium phosphate (α-TCP) is considered desirable for bone tissue engineering because of its relatively rapid degradation properties. However, such cement is relatively weak, restricting its use to areas of low mechanical stress. Wollastonite fibers (WF) have been used to improve the mechanical strength of biomaterials. However, the biological properties of WF remain poorly understood. Here, we tested the response of osteoblast-like cells to being cultured on CPC reinforced with 5% of WF (CPC-WF). We found that both types of cement studied achieved an ion balance for calcium and phosphate after 3 days of immersion in culture medium and this allowed subsequent long-term cell culture. CPC-WF increased cell viability and stimulated cell differentiation, compared to nonreinforced CPC. We hypothesize that late silicon release by CPC-WF induces increased cell proliferation and differentiation. Based on our findings, we propose that CPC-WF is a promising material for bone tissue engineering applications. |
| format | Article |
| id | doaj-art-168cdad64d234f34876758484ecdcb62 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-168cdad64d234f34876758484ecdcb622025-08-20T02:02:37ZengWileyThe Scientific World Journal2356-61401537-744X2017-01-01201710.1155/2017/52601065260106Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like CellsJuliana Almeida Domingues0Mariana Motisuke1Celso Aparecido Bertran2Moema A. Hausen3Eliana Aparecida de Rezende Duek4José Angelo Camilli5Department of Structural and Functional Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, SP, BrazilBioceramics Laboratory, Science and Technology Institute, Federal University of São Paulo (UNIFESP), São José dos Campos, SP, BrazilDepartment of Physical Chemistry, Chemistry Institute, University of Campinas (UNICAMP), Campinas, SP, BrazilDepartment of Physiological Science, Biomaterials Laboratory, Pontifical Catholic University of Sao Paulo (PUC-SP), Sorocaba, SP, BrazilDepartment of Physiological Science, Biomaterials Laboratory, Pontifical Catholic University of Sao Paulo (PUC-SP), Sorocaba, SP, BrazilDepartment of Structural and Functional Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, SP, BrazilCalcium phosphate cement (CPC) that is based on α-tricalcium phosphate (α-TCP) is considered desirable for bone tissue engineering because of its relatively rapid degradation properties. However, such cement is relatively weak, restricting its use to areas of low mechanical stress. Wollastonite fibers (WF) have been used to improve the mechanical strength of biomaterials. However, the biological properties of WF remain poorly understood. Here, we tested the response of osteoblast-like cells to being cultured on CPC reinforced with 5% of WF (CPC-WF). We found that both types of cement studied achieved an ion balance for calcium and phosphate after 3 days of immersion in culture medium and this allowed subsequent long-term cell culture. CPC-WF increased cell viability and stimulated cell differentiation, compared to nonreinforced CPC. We hypothesize that late silicon release by CPC-WF induces increased cell proliferation and differentiation. Based on our findings, we propose that CPC-WF is a promising material for bone tissue engineering applications.http://dx.doi.org/10.1155/2017/5260106 |
| spellingShingle | Juliana Almeida Domingues Mariana Motisuke Celso Aparecido Bertran Moema A. Hausen Eliana Aparecida de Rezende Duek José Angelo Camilli Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells The Scientific World Journal |
| title | Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells |
| title_full | Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells |
| title_fullStr | Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells |
| title_full_unstemmed | Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells |
| title_short | Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells |
| title_sort | addition of wollastonite fibers to calcium phosphate cement increases cell viability and stimulates differentiation of osteoblast like cells |
| url | http://dx.doi.org/10.1155/2017/5260106 |
| work_keys_str_mv | AT julianaalmeidadomingues additionofwollastonitefiberstocalciumphosphatecementincreasescellviabilityandstimulatesdifferentiationofosteoblastlikecells AT marianamotisuke additionofwollastonitefiberstocalciumphosphatecementincreasescellviabilityandstimulatesdifferentiationofosteoblastlikecells AT celsoaparecidobertran additionofwollastonitefiberstocalciumphosphatecementincreasescellviabilityandstimulatesdifferentiationofosteoblastlikecells AT moemaahausen additionofwollastonitefiberstocalciumphosphatecementincreasescellviabilityandstimulatesdifferentiationofosteoblastlikecells AT elianaaparecidaderezendeduek additionofwollastonitefiberstocalciumphosphatecementincreasescellviabilityandstimulatesdifferentiationofosteoblastlikecells AT joseangelocamilli additionofwollastonitefiberstocalciumphosphatecementincreasescellviabilityandstimulatesdifferentiationofosteoblastlikecells |