An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles
Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of H. pylori is of great significance for the prevention of gastric cancer. Herein, a sensitive electrochemical aptasensor using AgNPs-GO as redox probes was established for the specific detection of H. pyl...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1619336/full |
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| author | Xiaojuan You Xiaojuan You Xiaojuan You Xiaojuan You Mingyi Shao Mingyi Shao Huadong Wang Rui Zhu Xinwei Liu Lei Dong Yuesheng Gong Yongwei Li Yongwei Li |
| author_facet | Xiaojuan You Xiaojuan You Xiaojuan You Xiaojuan You Mingyi Shao Mingyi Shao Huadong Wang Rui Zhu Xinwei Liu Lei Dong Yuesheng Gong Yongwei Li Yongwei Li |
| author_sort | Xiaojuan You |
| collection | DOAJ |
| description | Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of H. pylori is of great significance for the prevention of gastric cancer. Herein, a sensitive electrochemical aptasensor using AgNPs-GO as redox probes was established for the specific detection of H. pylori in blood serum and stool samples. Firstly, AgNPs-GO nanocomposites were obtained by in-situ reduction of AgNPs on graphene oxide (GO) surface with glucose as reducing agent, in which AgNPs showed good biocompatibility and chemical stability, as well as redox property, and GO provided a large surface area to assemble a large number of AgNPs. Subsequently, the electrodeposition of AuNPs further improved the conductivity of the aptasensor. Additionally, the streptavidin was introduced into the aptasensor to effectively bind the biotin-modified aptamers. In this way, aptamers could be tethered to the surface through SA-biotin linkage, and H. pylori was selectivly binded by the aptamers subsequently. Under optimal conditions, the aptasensor could detect H. pylori in a wide concentration range (101 CFU mL−1–108 CFU mL−1) with a low detection limit of 3 CFU mL−1. What’s more, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other pathogens in clinical diagnosis. |
| format | Article |
| id | doaj-art-166c2faf2921490d8f3fb496d646c5f1 |
| institution | DOAJ |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-166c2faf2921490d8f3fb496d646c5f12025-08-20T03:17:24ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-07-011310.3389/fbioe.2025.16193361619336An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticlesXiaojuan You0Xiaojuan You1Xiaojuan You2Xiaojuan You3Mingyi Shao4Mingyi Shao5Huadong Wang6Rui Zhu7Xinwei Liu8Lei Dong9Yuesheng Gong10Yongwei Li11Yongwei Li12Central Laboratory, Zhengzhou Anorectal Hospital, Zhengzhou, ChinaThe First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou, ChinaThe Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaCentral Laboratory, Zhengzhou Anorectal Hospital, Zhengzhou, ChinaThe First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou, ChinaMedical Device Review Division I, Henan Center For Drug Evaluation and Inspection, Zhengzhou, ChinaThe Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, ChinaThe Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, ChinaCentral Laboratory, Zhengzhou Anorectal Hospital, Zhengzhou, ChinaCentral Laboratory, Zhengzhou Anorectal Hospital, Zhengzhou, ChinaCentral Laboratory, Zhengzhou Anorectal Hospital, Zhengzhou, ChinaThe Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, ChinaHelicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of H. pylori is of great significance for the prevention of gastric cancer. Herein, a sensitive electrochemical aptasensor using AgNPs-GO as redox probes was established for the specific detection of H. pylori in blood serum and stool samples. Firstly, AgNPs-GO nanocomposites were obtained by in-situ reduction of AgNPs on graphene oxide (GO) surface with glucose as reducing agent, in which AgNPs showed good biocompatibility and chemical stability, as well as redox property, and GO provided a large surface area to assemble a large number of AgNPs. Subsequently, the electrodeposition of AuNPs further improved the conductivity of the aptasensor. Additionally, the streptavidin was introduced into the aptasensor to effectively bind the biotin-modified aptamers. In this way, aptamers could be tethered to the surface through SA-biotin linkage, and H. pylori was selectivly binded by the aptamers subsequently. Under optimal conditions, the aptasensor could detect H. pylori in a wide concentration range (101 CFU mL−1–108 CFU mL−1) with a low detection limit of 3 CFU mL−1. What’s more, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other pathogens in clinical diagnosis.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1619336/fullHelicobacter pyloriAgNPs-GOAuNPsstreptavidinaptasensor |
| spellingShingle | Xiaojuan You Xiaojuan You Xiaojuan You Xiaojuan You Mingyi Shao Mingyi Shao Huadong Wang Rui Zhu Xinwei Liu Lei Dong Yuesheng Gong Yongwei Li Yongwei Li An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles Frontiers in Bioengineering and Biotechnology Helicobacter pylori AgNPs-GO AuNPs streptavidin aptasensor |
| title | An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles |
| title_full | An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles |
| title_fullStr | An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles |
| title_full_unstemmed | An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles |
| title_short | An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles |
| title_sort | electrochemical aptasensor for detection of helicobacter pylori based on aunps and agnps go nanoparticles |
| topic | Helicobacter pylori AgNPs-GO AuNPs streptavidin aptasensor |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1619336/full |
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