Assessing White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) concurrent with Vibrio spp. in various Penaeus monodon aquaculture farms at southwestern region of Bangladesh

This study was conducted to assess the prevalence of White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) in giant tiger shrimp (Penaeus monodon Fabricius, 1798) aquaculture systems (extensive, improved-extensive, and semi-intensive) in the southwestern region of Bang...

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Main Authors: Angkur Chowdhury, Chironjib Singha Samanta Chandan, Debasish Pandit, Basir Ahammad, Md. Habibur Rahman, Md. Imran Chowdhury, Rasel Mia, Shaila Akter, Md Zobayer Rahman, Bhaskar Chandra Majumdar
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Comparative Immunology Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950311624000454
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author Angkur Chowdhury
Chironjib Singha Samanta Chandan
Debasish Pandit
Basir Ahammad
Md. Habibur Rahman
Md. Imran Chowdhury
Rasel Mia
Shaila Akter
Md Zobayer Rahman
Bhaskar Chandra Majumdar
author_facet Angkur Chowdhury
Chironjib Singha Samanta Chandan
Debasish Pandit
Basir Ahammad
Md. Habibur Rahman
Md. Imran Chowdhury
Rasel Mia
Shaila Akter
Md Zobayer Rahman
Bhaskar Chandra Majumdar
author_sort Angkur Chowdhury
collection DOAJ
description This study was conducted to assess the prevalence of White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) in giant tiger shrimp (Penaeus monodon Fabricius, 1798) aquaculture systems (extensive, improved-extensive, and semi-intensive) in the southwestern region of Bangladesh. This research employed real-time polymerase chain reaction (RT-PCR) to quantification cycle (Cq) values for identification of WSSV and AHPND in shrimp samples and Vibrio spp. was identified through biochemical tests on water and soil samples (n = 72 for each) from different aquaculture systems. The results of the study indicated significant difference (p < 0.05) in nitrate and ammonia levels across the various aquaculture systems, highlighting the critical need for rigorous water quality monitoring and management. Extensive aquaculture exhibited the highest prevalence of WSSV (40 %) and AHPND (33 %) positive samples, whereas semi-intensive aquaculture had the lowest prevalence, with 22 % WSSV-positive and 20 % AHPND-positive samples. Biochemical analysis identified three Vibrio spp. such as V. cholerae, V. parahaemolyticus, and V. vulnificus - with significantly higher concentrations in extensive aquaculture compared to semi-intensive. Notably, V. vulnificus was more abundant (0.9 × 103 cfu/ml) in the soil of extensive aquaculture, while it was less prevalent in water. In conclusion, this study highlighted that the risk of WSSV and AHPND was greater in extensive aquaculture due to inadequate management.
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spelling doaj-art-166b67a9b86d4d9e8de66ab0df701ba12025-08-20T02:36:59ZengElsevierComparative Immunology Reports2950-31162024-12-01720017810.1016/j.cirep.2024.200178Assessing White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) concurrent with Vibrio spp. in various Penaeus monodon aquaculture farms at southwestern region of BangladeshAngkur Chowdhury0Chironjib Singha Samanta Chandan1Debasish Pandit2Basir Ahammad3Md. Habibur Rahman4Md. Imran Chowdhury5Rasel Mia6Shaila Akter7Md Zobayer Rahman8Bhaskar Chandra Majumdar9Department of Fishery Biology and Genetics, Khulna Agricultural University, Khulna 9100, Bangladesh; Corresponding authors.Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet 3100, BangladeshDepartment of Fishery Resources Conservation and Management, Khulna Agricultural University, Khulna 9100, BangladeshDepartment of Fish Health Management, Khulna Agricultural University, Khulna 9100, BangladeshDepartment of Oceanography, Khulna Agricultural University, Khulna 9100, BangladeshFisheries &amp; Marine Resource Technology Discipline, Khulna University, Khulna 9100, BangladeshDepartment of Aquatic Resource Management, Sylhet Agricultural University, Sylhet 3100, BangladeshDepartment of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet 3100, BangladeshDepartment of Fish Health Management, Sylhet Agricultural University, Sylhet 3100, BangladeshDepartment of Fisheries Technology and Quality Control, Khulna Agricultural University, Khulna 9100, Bangladesh; Corresponding authors.This study was conducted to assess the prevalence of White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) in giant tiger shrimp (Penaeus monodon Fabricius, 1798) aquaculture systems (extensive, improved-extensive, and semi-intensive) in the southwestern region of Bangladesh. This research employed real-time polymerase chain reaction (RT-PCR) to quantification cycle (Cq) values for identification of WSSV and AHPND in shrimp samples and Vibrio spp. was identified through biochemical tests on water and soil samples (n = 72 for each) from different aquaculture systems. The results of the study indicated significant difference (p < 0.05) in nitrate and ammonia levels across the various aquaculture systems, highlighting the critical need for rigorous water quality monitoring and management. Extensive aquaculture exhibited the highest prevalence of WSSV (40 %) and AHPND (33 %) positive samples, whereas semi-intensive aquaculture had the lowest prevalence, with 22 % WSSV-positive and 20 % AHPND-positive samples. Biochemical analysis identified three Vibrio spp. such as V. cholerae, V. parahaemolyticus, and V. vulnificus - with significantly higher concentrations in extensive aquaculture compared to semi-intensive. Notably, V. vulnificus was more abundant (0.9 × 103 cfu/ml) in the soil of extensive aquaculture, while it was less prevalent in water. In conclusion, this study highlighted that the risk of WSSV and AHPND was greater in extensive aquaculture due to inadequate management.http://www.sciencedirect.com/science/article/pii/S2950311624000454Peneaus monodonVibrio spp.WSSVAHPNDAquaculture systems
spellingShingle Angkur Chowdhury
Chironjib Singha Samanta Chandan
Debasish Pandit
Basir Ahammad
Md. Habibur Rahman
Md. Imran Chowdhury
Rasel Mia
Shaila Akter
Md Zobayer Rahman
Bhaskar Chandra Majumdar
Assessing White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) concurrent with Vibrio spp. in various Penaeus monodon aquaculture farms at southwestern region of Bangladesh
Comparative Immunology Reports
Peneaus monodon
Vibrio spp.
WSSV
AHPND
Aquaculture systems
title Assessing White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) concurrent with Vibrio spp. in various Penaeus monodon aquaculture farms at southwestern region of Bangladesh
title_full Assessing White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) concurrent with Vibrio spp. in various Penaeus monodon aquaculture farms at southwestern region of Bangladesh
title_fullStr Assessing White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) concurrent with Vibrio spp. in various Penaeus monodon aquaculture farms at southwestern region of Bangladesh
title_full_unstemmed Assessing White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) concurrent with Vibrio spp. in various Penaeus monodon aquaculture farms at southwestern region of Bangladesh
title_short Assessing White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) concurrent with Vibrio spp. in various Penaeus monodon aquaculture farms at southwestern region of Bangladesh
title_sort assessing white spot syndrome virus wssv and acute hepatopancreatic necrosis disease ahpnd concurrent with vibrio spp in various penaeus monodon aquaculture farms at southwestern region of bangladesh
topic Peneaus monodon
Vibrio spp.
WSSV
AHPND
Aquaculture systems
url http://www.sciencedirect.com/science/article/pii/S2950311624000454
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