ALPHA–2–MACROGLOBULIN COMPLEXES WITH IGG ANTIBODIES, PLASMIN, AND THEIR INTERRELATION WITH OTHER FACTORS OF HUMORAL IMMUNITY DURING THE DEVELOPMENT OF RHEUMATOID ARTHRITIS

Abstract. Human alpha–2–macroglobulin (α2–MG) acts as a broad–spectrum cytokine and proteasebinding protein, and it represents an evolutionarily conserved arm of innate immune system. Meanwhile, previous studies have shown that rheumatoid arthritis (RA) development may cause alterations in α2–MG con...

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Main Authors: V. N. Zorina, N. A. Trofimenko, S. V. Arkhipova, R. M. Zorina, N. A. Zorin
Format: Article
Language:Russian
Published: St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists 2014-07-01
Series:Медицинская иммунология
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Online Access:https://www.mimmun.ru/mimmun/article/view/623
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author V. N. Zorina
N. A. Trofimenko
S. V. Arkhipova
R. M. Zorina
N. A. Zorin
author_facet V. N. Zorina
N. A. Trofimenko
S. V. Arkhipova
R. M. Zorina
N. A. Zorin
author_sort V. N. Zorina
collection DOAJ
description Abstract. Human alpha–2–macroglobulin (α2–MG) acts as a broad–spectrum cytokine and proteasebinding protein, and it represents an evolutionarily conserved arm of innate immune system. Meanwhile, previous studies have shown that rheumatoid arthritis (RA) development may cause alterations in α2–MG conformation and lessen its ability to bind and utilise regulatory substances. We investigated serum contents of α2–MG–IgG and α2–MG–plasmin complexes, as well as total concentrations ofα2–MG, plasmin (Pl), IgG, IL–6, IL–1β, TNFα and Rf–IgM, in order to evaluate the levels of anti–α2–MG antibody production in sera of patients with different degrees of RA activity and some interrelations of preformed immune complex with some other substances implicated in RA development. Serum samples were obtained in acute phase of RA, before the treatment was started. We have revealed significantly increased levels of α2–MG–IgG complex in the groups with severe RA, accompanied by increase in total IgG levels, without significant changes in total α2–MG concentrations. We have also demonstrated that increased levels of Pl–α2–MG complex did correspond to the severity of disease, and showed statistically high correlation with α2–MG–IgG levels. We have found a significant increase of IL–1β, IL–6, TNFα and Rf–IgM in RA, in absence of significant correlations with α2–MG–IgG contents. The results obtained allow us to suggest that abundant accumulation of serum antibodies to α2–MG or Pl–α2–MG complex during inflammation in the people with innate α2–MG deficiency, followed by increased levels of proinflammatory cytokines, may provoke a cascade–like development of RA. Serum levels of α2–MG may serve as prognostic marker in RA. (Med. Immunol., 2005, vol.7, № 5–6, pp. 557–562)
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institution Kabale University
issn 1563-0625
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language Russian
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spelling doaj-art-166455d1a63f4861af33986282df9bb32025-08-20T04:00:09ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0175-655756210.15789/1563-0625-2005-5-6-557-562620ALPHA–2–MACROGLOBULIN COMPLEXES WITH IGG ANTIBODIES, PLASMIN, AND THEIR INTERRELATION WITH OTHER FACTORS OF HUMORAL IMMUNITY DURING THE DEVELOPMENT OF RHEUMATOID ARTHRITISV. N. Zorina0N. A. Trofimenko1S. V. Arkhipova2R. M. Zorina3N. A. Zorin4ГОУ ДПО Новокузнецкий государственный институт усовершенствования врачейПервая городская клиническая больница, г. НовокузнецкГОУ ДПО Новокузнецкий государственный институт усовершенствования врачейГОУ ДПО Новокузнецкий государственный институт усовершенствования врачейГОУ ДПО Новокузнецкий государственный институт усовершенствования врачейAbstract. Human alpha–2–macroglobulin (α2–MG) acts as a broad–spectrum cytokine and proteasebinding protein, and it represents an evolutionarily conserved arm of innate immune system. Meanwhile, previous studies have shown that rheumatoid arthritis (RA) development may cause alterations in α2–MG conformation and lessen its ability to bind and utilise regulatory substances. We investigated serum contents of α2–MG–IgG and α2–MG–plasmin complexes, as well as total concentrations ofα2–MG, plasmin (Pl), IgG, IL–6, IL–1β, TNFα and Rf–IgM, in order to evaluate the levels of anti–α2–MG antibody production in sera of patients with different degrees of RA activity and some interrelations of preformed immune complex with some other substances implicated in RA development. Serum samples were obtained in acute phase of RA, before the treatment was started. We have revealed significantly increased levels of α2–MG–IgG complex in the groups with severe RA, accompanied by increase in total IgG levels, without significant changes in total α2–MG concentrations. We have also demonstrated that increased levels of Pl–α2–MG complex did correspond to the severity of disease, and showed statistically high correlation with α2–MG–IgG levels. We have found a significant increase of IL–1β, IL–6, TNFα and Rf–IgM in RA, in absence of significant correlations with α2–MG–IgG contents. The results obtained allow us to suggest that abundant accumulation of serum antibodies to α2–MG or Pl–α2–MG complex during inflammation in the people with innate α2–MG deficiency, followed by increased levels of proinflammatory cytokines, may provoke a cascade–like development of RA. Serum levels of α2–MG may serve as prognostic marker in RA. (Med. Immunol., 2005, vol.7, № 5–6, pp. 557–562)https://www.mimmun.ru/mimmun/article/view/623alpha-2-macroglobulinrheumatoid arthritisautoantibodiescytokines
spellingShingle V. N. Zorina
N. A. Trofimenko
S. V. Arkhipova
R. M. Zorina
N. A. Zorin
ALPHA–2–MACROGLOBULIN COMPLEXES WITH IGG ANTIBODIES, PLASMIN, AND THEIR INTERRELATION WITH OTHER FACTORS OF HUMORAL IMMUNITY DURING THE DEVELOPMENT OF RHEUMATOID ARTHRITIS
Медицинская иммунология
alpha-2-macroglobulin
rheumatoid arthritis
autoantibodies
cytokines
title ALPHA–2–MACROGLOBULIN COMPLEXES WITH IGG ANTIBODIES, PLASMIN, AND THEIR INTERRELATION WITH OTHER FACTORS OF HUMORAL IMMUNITY DURING THE DEVELOPMENT OF RHEUMATOID ARTHRITIS
title_full ALPHA–2–MACROGLOBULIN COMPLEXES WITH IGG ANTIBODIES, PLASMIN, AND THEIR INTERRELATION WITH OTHER FACTORS OF HUMORAL IMMUNITY DURING THE DEVELOPMENT OF RHEUMATOID ARTHRITIS
title_fullStr ALPHA–2–MACROGLOBULIN COMPLEXES WITH IGG ANTIBODIES, PLASMIN, AND THEIR INTERRELATION WITH OTHER FACTORS OF HUMORAL IMMUNITY DURING THE DEVELOPMENT OF RHEUMATOID ARTHRITIS
title_full_unstemmed ALPHA–2–MACROGLOBULIN COMPLEXES WITH IGG ANTIBODIES, PLASMIN, AND THEIR INTERRELATION WITH OTHER FACTORS OF HUMORAL IMMUNITY DURING THE DEVELOPMENT OF RHEUMATOID ARTHRITIS
title_short ALPHA–2–MACROGLOBULIN COMPLEXES WITH IGG ANTIBODIES, PLASMIN, AND THEIR INTERRELATION WITH OTHER FACTORS OF HUMORAL IMMUNITY DURING THE DEVELOPMENT OF RHEUMATOID ARTHRITIS
title_sort alpha 2 macroglobulin complexes with igg antibodies plasmin and their interrelation with other factors of humoral immunity during the development of rheumatoid arthritis
topic alpha-2-macroglobulin
rheumatoid arthritis
autoantibodies
cytokines
url https://www.mimmun.ru/mimmun/article/view/623
work_keys_str_mv AT vnzorina alpha2macroglobulincomplexeswithiggantibodiesplasminandtheirinterrelationwithotherfactorsofhumoralimmunityduringthedevelopmentofrheumatoidarthritis
AT natrofimenko alpha2macroglobulincomplexeswithiggantibodiesplasminandtheirinterrelationwithotherfactorsofhumoralimmunityduringthedevelopmentofrheumatoidarthritis
AT svarkhipova alpha2macroglobulincomplexeswithiggantibodiesplasminandtheirinterrelationwithotherfactorsofhumoralimmunityduringthedevelopmentofrheumatoidarthritis
AT rmzorina alpha2macroglobulincomplexeswithiggantibodiesplasminandtheirinterrelationwithotherfactorsofhumoralimmunityduringthedevelopmentofrheumatoidarthritis
AT nazorin alpha2macroglobulincomplexeswithiggantibodiesplasminandtheirinterrelationwithotherfactorsofhumoralimmunityduringthedevelopmentofrheumatoidarthritis