Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat
Double haploids (DHs) production and utilization is an important aspect of wheat breeding programs in worldwide, because, it provides many advantages over the conventional breeding program. Therefore, in vitro and in vivo responses of anther derived two bread wheat cultivars and their F1 crosses wer...
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Ankara University
2022-09-01
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| Series: | Journal of Agricultural Sciences |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1395356 |
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| author | Surendra Barpete Sancar Fatih Özcan Güray Akdogan Hussein Abdullah Ahmed Ahmed |
| author_facet | Surendra Barpete Sancar Fatih Özcan Güray Akdogan Hussein Abdullah Ahmed Ahmed |
| author_sort | Surendra Barpete |
| collection | DOAJ |
| description | Double haploids (DHs) production and utilization is an important aspect of wheat breeding programs in worldwide, because, it provides many advantages over the conventional breeding program. Therefore, in vitro and in vivo responses of anther derived two bread wheat cultivars and their F1 crosses were investigated. Results showed significant genetic variation among the tested bread wheat genotypes. The pre-cold treated anther derived embryonic calli and shoot induction (%) were obtained from all genotypes that ranged 44 - 76% and 32 - 58% respectively. The highest shoot induction from embryonic calli was achieved from F1 cross of Zubkov x Atay-85, whereas, lowest induction was obtained from Atay-85 cultivar. The F1 cross (Zubkov x Atay-85) showed a better response in anther culture in term of shoots per plant and root induction than their respective parental genotypes.The present results indicated positive and significant heterotic effects of F1 crosses for calli and shoot induction that also showed less albino plant regeneration after colchicine treatments. Total number of 114 anthers driven DHs lines from two genotypes and their F1 crosses were regenerated, confirmed by flow-cytometry and evaluated under field condition. Some DHs lines were found to be significantly superior for agronomical traits including seed yield than the parental genotypes as well as local bread wheat (control) cultivars. |
| format | Article |
| id | doaj-art-165c41fb539b4e1f8661f06e9dd18418 |
| institution | OA Journals |
| issn | 1300-7580 2148-9297 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Ankara University |
| record_format | Article |
| series | Journal of Agricultural Sciences |
| spelling | doaj-art-165c41fb539b4e1f8661f06e9dd184182025-08-20T02:01:14ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972022-09-0128336337110.15832/ankutbd.82543445Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread WheatSurendra Barpete0Sancar Fatih Özcan1Güray Akdogan2Hussein Abdullah Ahmed Ahmed3ICARDA-Food Legume Research Platform, Amlaha, Sehore-466113, IndiaCentral Research Institute for Field Crops, Ministry of Food, Agriculture and Livestock, Ankara, TurkeyDepartment of Field Crops, Faculty of Agriculture, University of AnkaraDepartment of Field Crops, Faculty of Agriculture, University of AnkaraDouble haploids (DHs) production and utilization is an important aspect of wheat breeding programs in worldwide, because, it provides many advantages over the conventional breeding program. Therefore, in vitro and in vivo responses of anther derived two bread wheat cultivars and their F1 crosses were investigated. Results showed significant genetic variation among the tested bread wheat genotypes. The pre-cold treated anther derived embryonic calli and shoot induction (%) were obtained from all genotypes that ranged 44 - 76% and 32 - 58% respectively. The highest shoot induction from embryonic calli was achieved from F1 cross of Zubkov x Atay-85, whereas, lowest induction was obtained from Atay-85 cultivar. The F1 cross (Zubkov x Atay-85) showed a better response in anther culture in term of shoots per plant and root induction than their respective parental genotypes.The present results indicated positive and significant heterotic effects of F1 crosses for calli and shoot induction that also showed less albino plant regeneration after colchicine treatments. Total number of 114 anthers driven DHs lines from two genotypes and their F1 crosses were regenerated, confirmed by flow-cytometry and evaluated under field condition. Some DHs lines were found to be significantly superior for agronomical traits including seed yield than the parental genotypes as well as local bread wheat (control) cultivars.https://dergipark.org.tr/tr/download/article-file/1395356anther culturebread wheatcolchicinedouble haploidtriticum aestivum l., |
| spellingShingle | Surendra Barpete Sancar Fatih Özcan Güray Akdogan Hussein Abdullah Ahmed Ahmed Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat Journal of Agricultural Sciences anther culture bread wheat colchicine double haploid triticum aestivum l., |
| title | Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat |
| title_full | Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat |
| title_fullStr | Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat |
| title_full_unstemmed | Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat |
| title_short | Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat |
| title_sort | effect of parental genotypes and their reciprocal crosses on haploid plant production by anther culture and confirmation of double haploids by flow cytometry in bread wheat |
| topic | anther culture bread wheat colchicine double haploid triticum aestivum l., |
| url | https://dergipark.org.tr/tr/download/article-file/1395356 |
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