blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India
Introduction: The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. Methodology: A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudo...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2012-11-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/2268 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850149184898859008 |
|---|---|
| author | M Shanthi Amudhan Uma Sekar Arunagiri Kamalanathan Sekar Balaraman |
| author_facet | M Shanthi Amudhan Uma Sekar Arunagiri Kamalanathan Sekar Balaraman |
| author_sort | M Shanthi Amudhan |
| collection | DOAJ |
| description | Introduction: The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options.
Methodology: A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61), Acinetobacter baumannii (116), Acinetobacter lwoffii (1) and Pseudomonas stutzeri (1) isolated from patients hospitalized for 48 hours or more were included in the study. The minimum inhibitory concentrations (MIC) to imipenem and meropenem were determined and interpreted according to Clinical Laboratory Standards Institute guidelines. The Modified Hodge Test (MHT) and inhibitor potentiated disk diffusion tests with ethylenediaminetetraacetic acid (EDTA) were used for screening of carbapenamases and MBL production respectively. Polymerase chain reaction (PCR) was performed for the detection of MBL (blaVIM and blaIMP) genes. Gene sequencing was performed for representative isolates.
Results: MHT was positive in 94.4% (n = 169). MBL screening with EDTA was positive in 80.4% (n = 144). MBL genes bla VIM and bla IMP were detected in 92 (51.4%) isolates. Bla VIM alone was detected in 89 isolates while two isolates had bla IMP alone. One isolate had both bla VIM and bla IMP. Among the P. aeruginosa, 36 carried the MBL gene. In A. baumannii, 54 carried the MBL gene. Bla VIM was found in P. stutzeri and A. lwoffii isolates.
Conclusion: Carbapenem resistance in P. aeruginosa and A. baumannii is chiefly mediated by MBL production. The common MBL gene is the blaVIM.
|
| format | Article |
| id | doaj-art-165c30c4e9d2405f851c90cd5e6f249d |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2012-11-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-165c30c4e9d2405f851c90cd5e6f249d2025-08-20T02:27:00ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802012-11-0161110.3855/jidc.2268blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in IndiaM Shanthi Amudhan0Uma Sekar1Arunagiri Kamalanathan2Sekar Balaraman3Sri Ramachandra Medical College and Research Institute, Porur, Chennai, IndiaSri Ramachandra Medical College and Research Institute, Porur, Chennai, IndiaCentral Leprosy Teaching and Research Institute, Chengalpattu, Tamil Nadu, IndiaCentral Leprosy Teaching and Research Institute, Chengalpattu, Tamil Nadu, IndiaIntroduction: The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. Methodology: A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61), Acinetobacter baumannii (116), Acinetobacter lwoffii (1) and Pseudomonas stutzeri (1) isolated from patients hospitalized for 48 hours or more were included in the study. The minimum inhibitory concentrations (MIC) to imipenem and meropenem were determined and interpreted according to Clinical Laboratory Standards Institute guidelines. The Modified Hodge Test (MHT) and inhibitor potentiated disk diffusion tests with ethylenediaminetetraacetic acid (EDTA) were used for screening of carbapenamases and MBL production respectively. Polymerase chain reaction (PCR) was performed for the detection of MBL (blaVIM and blaIMP) genes. Gene sequencing was performed for representative isolates. Results: MHT was positive in 94.4% (n = 169). MBL screening with EDTA was positive in 80.4% (n = 144). MBL genes bla VIM and bla IMP were detected in 92 (51.4%) isolates. Bla VIM alone was detected in 89 isolates while two isolates had bla IMP alone. One isolate had both bla VIM and bla IMP. Among the P. aeruginosa, 36 carried the MBL gene. In A. baumannii, 54 carried the MBL gene. Bla VIM was found in P. stutzeri and A. lwoffii isolates. Conclusion: Carbapenem resistance in P. aeruginosa and A. baumannii is chiefly mediated by MBL production. The common MBL gene is the blaVIM. https://jidc.org/index.php/journal/article/view/2268carbapenemsmetallo-beta-lactamasespolymerase chain reaction |
| spellingShingle | M Shanthi Amudhan Uma Sekar Arunagiri Kamalanathan Sekar Balaraman blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India Journal of Infection in Developing Countries carbapenems metallo-beta-lactamases polymerase chain reaction |
| title | blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India |
| title_full | blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India |
| title_fullStr | blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India |
| title_full_unstemmed | blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India |
| title_short | blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India |
| title_sort | blaimp and blavim mediated carbapenem resistance in pseudomonas and acinetobacter species in india |
| topic | carbapenems metallo-beta-lactamases polymerase chain reaction |
| url | https://jidc.org/index.php/journal/article/view/2268 |
| work_keys_str_mv | AT mshanthiamudhan blaimpandblavimmediatedcarbapenemresistanceinpseudomonasandacinetobacterspeciesinindia AT umasekar blaimpandblavimmediatedcarbapenemresistanceinpseudomonasandacinetobacterspeciesinindia AT arunagirikamalanathan blaimpandblavimmediatedcarbapenemresistanceinpseudomonasandacinetobacterspeciesinindia AT sekarbalaraman blaimpandblavimmediatedcarbapenemresistanceinpseudomonasandacinetobacterspeciesinindia |