Case Report: Inflammation-Driven Species-Level Shifts in the Oral Microbiome of Refractory Feline Chronic Gingivostomatitis

The cat oral microbiome plays an important role in maintaining host health, yet little is known about how to apply microbial data in a clinical setting. One such use of microbiome signatures is in cases of feline chronic gingivostomatitis (FCGS), a severe debilitating complex disease of the oral cav...

Full description

Saved in:
Bibliographic Details
Main Authors: Claire A. Shaw, Maria Soltero-Rivera, Rodrigo Profeta, Bart C. Weimer
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Bacteria
Subjects:
Online Access:https://www.mdpi.com/2674-1334/4/1/1
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850090106031964160
author Claire A. Shaw
Maria Soltero-Rivera
Rodrigo Profeta
Bart C. Weimer
author_facet Claire A. Shaw
Maria Soltero-Rivera
Rodrigo Profeta
Bart C. Weimer
author_sort Claire A. Shaw
collection DOAJ
description The cat oral microbiome plays an important role in maintaining host health, yet little is known about how to apply microbial data in a clinical setting. One such use of microbiome signatures is in cases of feline chronic gingivostomatitis (FCGS), a severe debilitating complex disease of the oral cavity. FCGS-afflicted cats have limited treatment options, and individual patient responses to treatment are needed. In this work, we used deep sequencing of total RNA of the oral microbiome to chronicle microbial changes that accompanied an FCGS-afflicted cat’s change from treatment-non-responsive to treatment-responsive within a 17-month span. The oral microbiome composition of the two treatment-non-responsive time points differed from that of the treatment-responsive point, with notable shifts in the abundance of <i>Myscoplasmopsis</i>, <i>Aspergillus</i>, and <i>Capnocytophaga</i> species. Intriguingly, the presence of the fungal groups <i>Aspergillus</i> and <i>Candida</i> primarily differentiated the two non-responsive microbiomes. Associated with responder status were multiple <i>Capnocytophaga</i> species, including <i>Capnocytophaga</i> sp. H2931, <i>Capnocytophaga gingivalis</i>, and <i>Capnocytophaga canimorsus</i>. The observation that the oral microbiome shifts in tandem by response to treatment in FCGS suggests a potential use for microbiome evaluations in a clinical setting. This work contributes to developing improved molecular diagnostics for enhanced efficacy of individualized treatment plans to improve oral disease.
format Article
id doaj-art-164bf3e022014e4b8c80213314a01cfe
institution DOAJ
issn 2674-1334
language English
publishDate 2025-01-01
publisher MDPI AG
record_format Article
series Bacteria
spelling doaj-art-164bf3e022014e4b8c80213314a01cfe2025-08-20T02:42:38ZengMDPI AGBacteria2674-13342025-01-0141110.3390/bacteria4010001Case Report: Inflammation-Driven Species-Level Shifts in the Oral Microbiome of Refractory Feline Chronic GingivostomatitisClaire A. Shaw0Maria Soltero-Rivera1Rodrigo Profeta2Bart C. Weimer3Department of Population Health and Reproduction, 100K Pathogen Genome Project, University of California, Davis, CA 95616, USADepartment of Surgical and Radiological Sciences, University of California, Davis, CA 95616, USADepartment of Population Health and Reproduction, 100K Pathogen Genome Project, University of California, Davis, CA 95616, USADepartment of Population Health and Reproduction, 100K Pathogen Genome Project, University of California, Davis, CA 95616, USAThe cat oral microbiome plays an important role in maintaining host health, yet little is known about how to apply microbial data in a clinical setting. One such use of microbiome signatures is in cases of feline chronic gingivostomatitis (FCGS), a severe debilitating complex disease of the oral cavity. FCGS-afflicted cats have limited treatment options, and individual patient responses to treatment are needed. In this work, we used deep sequencing of total RNA of the oral microbiome to chronicle microbial changes that accompanied an FCGS-afflicted cat’s change from treatment-non-responsive to treatment-responsive within a 17-month span. The oral microbiome composition of the two treatment-non-responsive time points differed from that of the treatment-responsive point, with notable shifts in the abundance of <i>Myscoplasmopsis</i>, <i>Aspergillus</i>, and <i>Capnocytophaga</i> species. Intriguingly, the presence of the fungal groups <i>Aspergillus</i> and <i>Candida</i> primarily differentiated the two non-responsive microbiomes. Associated with responder status were multiple <i>Capnocytophaga</i> species, including <i>Capnocytophaga</i> sp. H2931, <i>Capnocytophaga gingivalis</i>, and <i>Capnocytophaga canimorsus</i>. The observation that the oral microbiome shifts in tandem by response to treatment in FCGS suggests a potential use for microbiome evaluations in a clinical setting. This work contributes to developing improved molecular diagnostics for enhanced efficacy of individualized treatment plans to improve oral disease.https://www.mdpi.com/2674-1334/4/1/1feline chronic gingivostomatitismetagenomicsoral microbiome
spellingShingle Claire A. Shaw
Maria Soltero-Rivera
Rodrigo Profeta
Bart C. Weimer
Case Report: Inflammation-Driven Species-Level Shifts in the Oral Microbiome of Refractory Feline Chronic Gingivostomatitis
Bacteria
feline chronic gingivostomatitis
metagenomics
oral microbiome
title Case Report: Inflammation-Driven Species-Level Shifts in the Oral Microbiome of Refractory Feline Chronic Gingivostomatitis
title_full Case Report: Inflammation-Driven Species-Level Shifts in the Oral Microbiome of Refractory Feline Chronic Gingivostomatitis
title_fullStr Case Report: Inflammation-Driven Species-Level Shifts in the Oral Microbiome of Refractory Feline Chronic Gingivostomatitis
title_full_unstemmed Case Report: Inflammation-Driven Species-Level Shifts in the Oral Microbiome of Refractory Feline Chronic Gingivostomatitis
title_short Case Report: Inflammation-Driven Species-Level Shifts in the Oral Microbiome of Refractory Feline Chronic Gingivostomatitis
title_sort case report inflammation driven species level shifts in the oral microbiome of refractory feline chronic gingivostomatitis
topic feline chronic gingivostomatitis
metagenomics
oral microbiome
url https://www.mdpi.com/2674-1334/4/1/1
work_keys_str_mv AT claireashaw casereportinflammationdrivenspecieslevelshiftsintheoralmicrobiomeofrefractoryfelinechronicgingivostomatitis
AT mariasolterorivera casereportinflammationdrivenspecieslevelshiftsintheoralmicrobiomeofrefractoryfelinechronicgingivostomatitis
AT rodrigoprofeta casereportinflammationdrivenspecieslevelshiftsintheoralmicrobiomeofrefractoryfelinechronicgingivostomatitis
AT bartcweimer casereportinflammationdrivenspecieslevelshiftsintheoralmicrobiomeofrefractoryfelinechronicgingivostomatitis