High Mobility Group Box 1 Protein Induction by Mycobacterium Bovis BCG
High mobility group box 1 protein (HMGB1), a nuclear protein, is a critical cytokine that mediates the response to infection, injury, and inflammation. The aim of our study was to elaborate a reliable in vitro model to investigate whether Mycobacterium bovis BCG is able to induce HMGB1...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2007-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2007/53805 |
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| Summary: | High mobility group box 1 protein (HMGB1), a nuclear protein, is a critical cytokine that mediates the
response to infection, injury, and inflammation. The aim of our study was to elaborate a reliable in vitro
model to investigate whether Mycobacterium bovis BCG is able to induce HMGB1
secretion from the monocytic U-937 cells. Western blot technique was applied for the detection of HMGB1
from supernatants of cells, following induction with Mycobacterium bovis BCG. Densitometric
analysis revealed higher concentrations of HMGB1 in cell supernatants stimulated with BCG than in
the supernatants of the control, nonstimulated cells. Further quantitation of the secreted HMGB1 was
performed by ELISA. The BCG strain resulted in a higher amount of secreted HMGB1 (450 ± 44 ng/mL) than that of LPS (84 ± 12 ng/mL) or Staphylococcus aureus (150 ± 14 ng/mL). BCG and Phorbol −12-myristate −13 acetate (PMA), added together, resulted in the highest HMGB1 secretion (645 ± 125 ng/mL).
The translocation of the HMGB1 towards the cytoplasm following infection of cells with BCG was
demonstrated by immunofluorescence examinations.
Conclusion: Our pilot experiments draw attention to the HMGB1 inducing
ability of Mycobacterium bovis. Assesment of the pathophysiological role of this late
cytokine in mycobacterial infections demands further in vitro and in vivo examinations. |
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| ISSN: | 0962-9351 1466-1861 |