All-Trans Retinoic Acid Drives Development Phase- Specific Response to Adipogenic and Myogenic Processes in Bovine Skeletal Muscle-Derived Cells
Optimal muscle and intramuscular fat development are foundational to enhanced high-quality meat production in beef cattle, involving the proliferation and differentiation of key cellular populations, such as myoblasts and preadipocytes. Vitamin A is an essential nutrient for beef cattle and plays an...
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| Language: | English |
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Iowa State University Digital Press
2024-02-01
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| Series: | Meat and Muscle Biology |
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| Online Access: | https://www.iastatedigitalpress.com/mmb/article/id/16978/ |
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| author | Brad Kim DongQiao Peng HongGu Lee JinSoo Park JunHee Lee Steve B Smith WonSeob Kim XueCheng Jin |
| author_facet | Brad Kim DongQiao Peng HongGu Lee JinSoo Park JunHee Lee Steve B Smith WonSeob Kim XueCheng Jin |
| author_sort | Brad Kim |
| collection | DOAJ |
| description | Optimal muscle and intramuscular fat development are foundational to enhanced high-quality meat production in beef cattle, involving the proliferation and differentiation of key cellular populations, such as myoblasts and preadipocytes. Vitamin A is an essential nutrient for beef cattle and plays an important role in the formation and maintenance of numerous tissue types such as adipose tissue and skeletal muscle. The objective of this study was to investigate the effect of all-trans retinoic acid (ATRA), the active form of vitamin A, on adipogenic and myogenic processes in bovine skeletal muscle-derived cells (BSMC). As a result, ATRA treatment significantly upregulated ZFP423 and MYF5 during the growth phase (P<0.05). Moreover, ATRA treatment in the growth phase significantly elevated mRNA expression markers,including FABP4 and PPARγ in adipogenesis (P<0.05), as well as MYOD, MYOG, and MYF6 in myogenesis (P<0.05). In addition, when used only in the differentiation phase, ATRA treatment significantly increased the mRNA expression of myogenesis markers (MYOD, MYOG, and MYF6) (P<0.05) but decreased the mRNA expression of adipogenesis markers (FABP4 and PPARγ) compared with the control group (P<0.05). In conclusion, the ATRA treatment in the growth phase of BSMC indirectly promoted hyperplasia of myoblasts and preadipocytes, and the effect continued into subsequent differentiations. However, ATRA treatment plays a different role in adipogenic and myogenic differentiation by regulating related mRNA. These results will provide a reference for the application of vitamin A from the fetal to the postnatal period in beef cattle production. |
| format | Article |
| id | doaj-art-1645ec9889ac4cd7b215d65e853a8619 |
| institution | DOAJ |
| issn | 2575-985X |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Iowa State University Digital Press |
| record_format | Article |
| series | Meat and Muscle Biology |
| spelling | doaj-art-1645ec9889ac4cd7b215d65e853a86192025-08-20T02:45:11ZengIowa State University Digital PressMeat and Muscle Biology2575-985X2024-02-018110.22175/mmb.16978All-Trans Retinoic Acid Drives Development Phase- Specific Response to Adipogenic and Myogenic Processes in Bovine Skeletal Muscle-Derived CellsBrad Kim0https://orcid.org/0000-0003-1483-4391DongQiao Peng1https://orcid.org/0000-0002-0408-0381HongGu Lee2JinSoo Park3JunHee Lee4Steve B Smith5WonSeob Kim6XueCheng Jin7Department of Animal Sciences, Purdue UniversityCollege of Animal Sciences, Jilin universityDepartment of Animal Science and Technology, Konkuk universityDepartment of Animal Science and Technology, Konkuk universityDepartment of Animal Science and Technology, Konkuk universityAnimal Science, Texas A&M UniversityDepartment of Animal Science, Michigan State UniversityKonkuk universityOptimal muscle and intramuscular fat development are foundational to enhanced high-quality meat production in beef cattle, involving the proliferation and differentiation of key cellular populations, such as myoblasts and preadipocytes. Vitamin A is an essential nutrient for beef cattle and plays an important role in the formation and maintenance of numerous tissue types such as adipose tissue and skeletal muscle. The objective of this study was to investigate the effect of all-trans retinoic acid (ATRA), the active form of vitamin A, on adipogenic and myogenic processes in bovine skeletal muscle-derived cells (BSMC). As a result, ATRA treatment significantly upregulated ZFP423 and MYF5 during the growth phase (P<0.05). Moreover, ATRA treatment in the growth phase significantly elevated mRNA expression markers,including FABP4 and PPARγ in adipogenesis (P<0.05), as well as MYOD, MYOG, and MYF6 in myogenesis (P<0.05). In addition, when used only in the differentiation phase, ATRA treatment significantly increased the mRNA expression of myogenesis markers (MYOD, MYOG, and MYF6) (P<0.05) but decreased the mRNA expression of adipogenesis markers (FABP4 and PPARγ) compared with the control group (P<0.05). In conclusion, the ATRA treatment in the growth phase of BSMC indirectly promoted hyperplasia of myoblasts and preadipocytes, and the effect continued into subsequent differentiations. However, ATRA treatment plays a different role in adipogenic and myogenic differentiation by regulating related mRNA. These results will provide a reference for the application of vitamin A from the fetal to the postnatal period in beef cattle production.https://www.iastatedigitalpress.com/mmb/article/id/16978/adipogenic processall-trans retinoic acidmyogenic processzinc finger protein 423bovine skeletal muscle-derived cells |
| spellingShingle | Brad Kim DongQiao Peng HongGu Lee JinSoo Park JunHee Lee Steve B Smith WonSeob Kim XueCheng Jin All-Trans Retinoic Acid Drives Development Phase- Specific Response to Adipogenic and Myogenic Processes in Bovine Skeletal Muscle-Derived Cells Meat and Muscle Biology adipogenic process all-trans retinoic acid myogenic process zinc finger protein 423 bovine skeletal muscle-derived cells |
| title | All-Trans Retinoic Acid Drives Development Phase- Specific Response to Adipogenic and Myogenic Processes in Bovine Skeletal Muscle-Derived Cells |
| title_full | All-Trans Retinoic Acid Drives Development Phase- Specific Response to Adipogenic and Myogenic Processes in Bovine Skeletal Muscle-Derived Cells |
| title_fullStr | All-Trans Retinoic Acid Drives Development Phase- Specific Response to Adipogenic and Myogenic Processes in Bovine Skeletal Muscle-Derived Cells |
| title_full_unstemmed | All-Trans Retinoic Acid Drives Development Phase- Specific Response to Adipogenic and Myogenic Processes in Bovine Skeletal Muscle-Derived Cells |
| title_short | All-Trans Retinoic Acid Drives Development Phase- Specific Response to Adipogenic and Myogenic Processes in Bovine Skeletal Muscle-Derived Cells |
| title_sort | all trans retinoic acid drives development phase specific response to adipogenic and myogenic processes in bovine skeletal muscle derived cells |
| topic | adipogenic process all-trans retinoic acid myogenic process zinc finger protein 423 bovine skeletal muscle-derived cells |
| url | https://www.iastatedigitalpress.com/mmb/article/id/16978/ |
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