Cloning and sequence analysis of Hoxa-11a gene from zebrafish (Danio rerio)
Based on the published sequence data, coding sequence of zebrafish Hoxa-11a gene was amplified by PCR, cloned and sequenced. The acquired Hoxa-11a gene, which was comprised of 1581 bp, including 622 bp exon 1726 bp intron and 233 bp exon 2, encoding 284 amino acids. Compared with Hoxa-11 proteins fr...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2003-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.04.0433 |
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| Summary: | Based on the published sequence data, coding sequence of zebrafish Hoxa-11a gene was amplified by PCR, cloned and sequenced. The acquired Hoxa-11a gene, which was comprised of 1581 bp, including 622 bp exon 1726 bp intron and 233 bp exon 2, encoding 284 amino acids. Compared with Hoxa-11 proteins from human, mouse, chick, clawed frog, and coelacanth, homologies ware 50.0%, 51.3%, 53.3%, 56.7% and 59.7%, respectively. Besides the homeobox, two highly conserved regions in exon 1 and two highly conserved DNA sequences in intron were found. The size variation of Hoxa-11a genes among varous animals is inferred to be primarily caused by accumulation of alanine repeats and of flanking segments rich in glycine and serine in exon 1. |
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| ISSN: | 1008-9209 2097-5155 |