OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice
Abstract Clade A type 2C protein phosphatases (PP2Cs) are crucial components of the abscisic acid (ABA) signaling pathway. Research on clade A PP2Cs has focused more on their roles related to ABA signaling and stress responses than on the molecular mechanisms mediating their effects on plant growth...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-10-01
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| Series: | Rice |
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| Online Access: | https://doi.org/10.1186/s12284-024-00746-4 |
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| author | Jun Miao Liubing Bu Wenchen Tan Ping Wang Xiangbo Li Xianfeng Li Chuyan Chen Kunming Zhang Wenle Shen Zhiyun Gong Gulinaer Bahetibieke Lei Ren Guohua Liang Yong Zhou |
| author_facet | Jun Miao Liubing Bu Wenchen Tan Ping Wang Xiangbo Li Xianfeng Li Chuyan Chen Kunming Zhang Wenle Shen Zhiyun Gong Gulinaer Bahetibieke Lei Ren Guohua Liang Yong Zhou |
| author_sort | Jun Miao |
| collection | DOAJ |
| description | Abstract Clade A type 2C protein phosphatases (PP2Cs) are crucial components of the abscisic acid (ABA) signaling pathway. Research on clade A PP2Cs has focused more on their roles related to ABA signaling and stress responses than on the molecular mechanisms mediating their effects on plant growth and grain yield. Rice (Oryza sativa L.) is an important food crop worldwide. We previously determined that OsPP2C49, which encodes a rice clade A PP2C family member, negatively controls rice responses to drought, salt, and high-temperature stresses. In this study, we investigated the regulatory effects of OsPP2C49 on ABA responses and rice grain yield. By analyzing potential interactions with core ABA components, including pyrabactin resistance 1 (PYR1)/PYR1-like (PYL)/regulatory component of the ABA receptor (RCAR) and stress-activated protein kinases (SAPKs), we confirmed that OsPP2C49 is involved in the ABA signaling pathway. OsPP2C49 overexpression led to decreased ABA sensitivity and increased rice grain yield; the opposite phenotypes were observed in the ospp2c49 knockout mutants. Therefore, OsPP2C49 negatively regulates ABA responses, but positively modulates rice grain yield. Furthermore, we found that OsPP2C49 can interact with and dephosphorylate five OsSAPKs in vitro. Unlike OsPP2C49, these OsSAPKs positively modulate ABA responsiveness, but negatively affect rice yield. These findings indicate that OsPP2C49 may partially regulate ABA responses and rice grain production by dephosphorylating OsSAPKs. This study preliminarily explored the molecular basis of the regulatory effects of OsPP2C49 on rice plant growth and grain yield. |
| format | Article |
| id | doaj-art-16329d5ece4246b2aefd73327c2e7703 |
| institution | OA Journals |
| issn | 1939-8425 1939-8433 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj-art-16329d5ece4246b2aefd73327c2e77032025-08-20T02:11:47ZengSpringerOpenRice1939-84251939-84332024-10-0117111510.1186/s12284-024-00746-4OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in RiceJun Miao0Liubing Bu1Wenchen Tan2Ping Wang3Xiangbo Li4Xianfeng Li5Chuyan Chen6Kunming Zhang7Wenle Shen8Zhiyun Gong9Gulinaer Bahetibieke10Lei Ren11Guohua Liang12Yong Zhou13Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityIli Kazakh Autonomous Prefecture Institute of Agricultural ScienceIli Kazakh Autonomous Prefecture Institute of Agricultural ScienceJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityJiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Zhongshan Biological Breeding Laboratory, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou UniversityAbstract Clade A type 2C protein phosphatases (PP2Cs) are crucial components of the abscisic acid (ABA) signaling pathway. Research on clade A PP2Cs has focused more on their roles related to ABA signaling and stress responses than on the molecular mechanisms mediating their effects on plant growth and grain yield. Rice (Oryza sativa L.) is an important food crop worldwide. We previously determined that OsPP2C49, which encodes a rice clade A PP2C family member, negatively controls rice responses to drought, salt, and high-temperature stresses. In this study, we investigated the regulatory effects of OsPP2C49 on ABA responses and rice grain yield. By analyzing potential interactions with core ABA components, including pyrabactin resistance 1 (PYR1)/PYR1-like (PYL)/regulatory component of the ABA receptor (RCAR) and stress-activated protein kinases (SAPKs), we confirmed that OsPP2C49 is involved in the ABA signaling pathway. OsPP2C49 overexpression led to decreased ABA sensitivity and increased rice grain yield; the opposite phenotypes were observed in the ospp2c49 knockout mutants. Therefore, OsPP2C49 negatively regulates ABA responses, but positively modulates rice grain yield. Furthermore, we found that OsPP2C49 can interact with and dephosphorylate five OsSAPKs in vitro. Unlike OsPP2C49, these OsSAPKs positively modulate ABA responsiveness, but negatively affect rice yield. These findings indicate that OsPP2C49 may partially regulate ABA responses and rice grain production by dephosphorylating OsSAPKs. This study preliminarily explored the molecular basis of the regulatory effects of OsPP2C49 on rice plant growth and grain yield.https://doi.org/10.1186/s12284-024-00746-4RiceABAType 2C protein phosphataseGrain yield |
| spellingShingle | Jun Miao Liubing Bu Wenchen Tan Ping Wang Xiangbo Li Xianfeng Li Chuyan Chen Kunming Zhang Wenle Shen Zhiyun Gong Gulinaer Bahetibieke Lei Ren Guohua Liang Yong Zhou OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice Rice Rice ABA Type 2C protein phosphatase Grain yield |
| title | OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice |
| title_full | OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice |
| title_fullStr | OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice |
| title_full_unstemmed | OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice |
| title_short | OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice |
| title_sort | ospp2c49 a negative regulatory factor in the abscisic acid signaling pathway positively regulates grain yield in rice |
| topic | Rice ABA Type 2C protein phosphatase Grain yield |
| url | https://doi.org/10.1186/s12284-024-00746-4 |
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