Cefoperazone Sodium Content Assay in Pharmaceutical Formulations: Development and Validation of High-Performance Liquid Chromatographic Method
A precise and sensitive RP-HPLC method is used to assay pharmaceutical dosage forms and bulk drugs for cefoperazone sodium (CPZ). The mobile phase for cefoperazone separation is a combination of acetonitrile and 0.05 M ammonium acetate buffer (pH 4.5) in a 70 : 30 (v/v) ratio. This was separated by...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2024/6680231 |
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| Summary: | A precise and sensitive RP-HPLC method is used to assay pharmaceutical dosage forms and bulk drugs for cefoperazone sodium (CPZ). The mobile phase for cefoperazone separation is a combination of acetonitrile and 0.05 M ammonium acetate buffer (pH 4.5) in a 70 : 30 (v/v) ratio. This was separated by using a Waters XTerra RP-18 column (5 µm 250 × 4.6 mm internal diameter) that was working at a flow rate of 1.0 ml/min. Photodiode array detectors are able to set it at 235 nm. Based on the percentage recovery, the process produced a notable linear response ranging from 0.2 to 20 µg/ml, with a significant precision of 0.27 to 0.67% and an excellent accuracy of 99.90 to 100.02%. Because of its outstanding sensitivity, accuracy, precision, and ease of use, we highly suggest using the reversed-phase HPLC method. Therefore, a laboratory could utilize this method for routine quality control analysis. |
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| ISSN: | 2090-9071 |