Thermal Inactivation of Salmonella enterica and Listeria monocytogenes in Quesillo Manufactured from Raw Milk

Quesillo is an artisanal Honduran cheese made from raw milk. During fabrication, curd melting is considered a killing step for pathogenic bacteria. This work was aimed at determining the survival of Salmonella enterica and Listeria monocytogenes on inoculated curd packaged in plastic bags and immers...

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Main Authors: Mayra Márquez-González, Luis F. Osorio, Carmen G. Velásquez-Moreno, Alvaro G. García-Lira
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:International Journal of Food Science
Online Access:http://dx.doi.org/10.1155/2022/2507867
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author Mayra Márquez-González
Luis F. Osorio
Carmen G. Velásquez-Moreno
Alvaro G. García-Lira
author_facet Mayra Márquez-González
Luis F. Osorio
Carmen G. Velásquez-Moreno
Alvaro G. García-Lira
author_sort Mayra Márquez-González
collection DOAJ
description Quesillo is an artisanal Honduran cheese made from raw milk. During fabrication, curd melting is considered a killing step for pathogenic bacteria. This work was aimed at determining the survival of Salmonella enterica and Listeria monocytogenes on inoculated curd packaged in plastic bags and immersed in a water bath at 48, 54, 60, 65, and 70°C for predetermined times. Survival counts of each pathogen were used to estimate D values by linear regression, and z values were estimated by the linear regression of the D values. S. entericaD values ranged from 4.5 min at 60°C to 0.80 min at 70°C (z=10.7°C). For L. monocytogenes, D values ranged from 6.08 min at 60°C to 0.90 min at 70°C (z=11.3°C). Validation of 7-log reduction was performed on inoculated curd heated at 65°C for 34.7 min, and recovering enrichment procedures were used for each pathogen. Neither S. enterica nor L. monocytogenes cells were recovered after the enrichment of samples. The results obtained in this study could be applied by Honduran quesillo processors to improve the safety of their products.
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institution OA Journals
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publishDate 2022-01-01
publisher Wiley
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series International Journal of Food Science
spelling doaj-art-1603977835cc4d2fa8fab9f7dac409a82025-08-20T02:02:36ZengWileyInternational Journal of Food Science2314-57652022-01-01202210.1155/2022/2507867Thermal Inactivation of Salmonella enterica and Listeria monocytogenes in Quesillo Manufactured from Raw MilkMayra Márquez-González0Luis F. Osorio1Carmen G. Velásquez-Moreno2Alvaro G. García-Lira3Food Science and Technology DepartmentEARTH UniversityFood Science and Technology DepartmentFood Science and Technology DepartmentQuesillo is an artisanal Honduran cheese made from raw milk. During fabrication, curd melting is considered a killing step for pathogenic bacteria. This work was aimed at determining the survival of Salmonella enterica and Listeria monocytogenes on inoculated curd packaged in plastic bags and immersed in a water bath at 48, 54, 60, 65, and 70°C for predetermined times. Survival counts of each pathogen were used to estimate D values by linear regression, and z values were estimated by the linear regression of the D values. S. entericaD values ranged from 4.5 min at 60°C to 0.80 min at 70°C (z=10.7°C). For L. monocytogenes, D values ranged from 6.08 min at 60°C to 0.90 min at 70°C (z=11.3°C). Validation of 7-log reduction was performed on inoculated curd heated at 65°C for 34.7 min, and recovering enrichment procedures were used for each pathogen. Neither S. enterica nor L. monocytogenes cells were recovered after the enrichment of samples. The results obtained in this study could be applied by Honduran quesillo processors to improve the safety of their products.http://dx.doi.org/10.1155/2022/2507867
spellingShingle Mayra Márquez-González
Luis F. Osorio
Carmen G. Velásquez-Moreno
Alvaro G. García-Lira
Thermal Inactivation of Salmonella enterica and Listeria monocytogenes in Quesillo Manufactured from Raw Milk
International Journal of Food Science
title Thermal Inactivation of Salmonella enterica and Listeria monocytogenes in Quesillo Manufactured from Raw Milk
title_full Thermal Inactivation of Salmonella enterica and Listeria monocytogenes in Quesillo Manufactured from Raw Milk
title_fullStr Thermal Inactivation of Salmonella enterica and Listeria monocytogenes in Quesillo Manufactured from Raw Milk
title_full_unstemmed Thermal Inactivation of Salmonella enterica and Listeria monocytogenes in Quesillo Manufactured from Raw Milk
title_short Thermal Inactivation of Salmonella enterica and Listeria monocytogenes in Quesillo Manufactured from Raw Milk
title_sort thermal inactivation of salmonella enterica and listeria monocytogenes in quesillo manufactured from raw milk
url http://dx.doi.org/10.1155/2022/2507867
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AT carmengvelasquezmoreno thermalinactivationofsalmonellaentericaandlisteriamonocytogenesinquesillomanufacturedfromrawmilk
AT alvaroggarcialira thermalinactivationofsalmonellaentericaandlisteriamonocytogenesinquesillomanufacturedfromrawmilk