Tumor-associated mesenchymal stem cells in chemically-induced breast cancer in Wistar rats
Objective: to compare the morphological and functional properties of mesenchymal stem cells from mammary tissues and chemically-induced mammary tumor tissues. material and methods. The study included 25 female Wistar rats. In 20 rats, mammary carcinoma was induced by intramammary injection of N-meth...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Russian Academy of Sciences, Tomsk National Research Medical Center
2019-02-01
|
| Series: | Сибирский онкологический журнал |
| Subjects: | |
| Online Access: | https://www.siboncoj.ru/jour/article/view/960 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850030700616482816 |
|---|---|
| author | A. P. Lykov A. V. Kabakov N. A. Bondarenko O. V. Poveshchenko T. V. Rayter O. V. Kazakov D. N. Strunkin M. A. Surovtseva A. F. Poveshchenko V. I. Konenkov |
| author_facet | A. P. Lykov A. V. Kabakov N. A. Bondarenko O. V. Poveshchenko T. V. Rayter O. V. Kazakov D. N. Strunkin M. A. Surovtseva A. F. Poveshchenko V. I. Konenkov |
| author_sort | A. P. Lykov |
| collection | DOAJ |
| description | Objective: to compare the morphological and functional properties of mesenchymal stem cells from mammary tissues and chemically-induced mammary tumor tissues. material and methods. The study included 25 female Wistar rats. In 20 rats, mammary carcinoma was induced by intramammary injection of N-methyl-N-nitrosourea after estrus synchronization with chorionic gonadotropin. The control group consisted of 5 rats. Mammary carcinoma was verified histologically and immunohistochemically. To examine, whether the cells isolated from normal tissue and tumor tissue belonged to mesenchymal stem cells, FACS Canto II flow cytofluorometer was used. The functional properties of mesenchymal stem cells were evaluated in MTT assay by the level of nitric oxide production in normal and by hydrogen peroxide-induced hypoxia. The levels of prolactin, luteinizing hormone and estradiol E2 in urine were studied using solid-phase enzyme-linked immunosorbent assay. results. Chemically-induced mammary tumor according to histological and immunohistochemical studies corresponded to luminal B type breast cancer in humans. In rats that developed mammary tumors, the urine prolactin levels after synchronization of estrus were increased. In rats that did not develop tumors, the levels of prolactin and luteinizing hormone were decreased, but the levels of estradiol E2 were increased. More mesenchymal stem cells with CD45-/CD90+phenotype were obtained from the breast tumor tissue. Mesenchymal stem cells from tumor tissue showed increased proliferative potential and were more resistant to hypoxia. conclusion. Tumor- associated mesenchymal stem cells having high proliferative potential and resistance to hypoxia were obtained from chemically-induced mammary tumor tissue. Morphologic and functional differences in mesenchymal stem cells obtained from mammary breast tissue and tumor tissue require further studies. |
| format | Article |
| id | doaj-art-1601e87c982d48dea826afaf63f0adf8 |
| institution | DOAJ |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2019-02-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-1601e87c982d48dea826afaf63f0adf82025-08-20T02:59:08ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682019-02-01181566410.21294/1814-4861-2019-18-1-56-64612Tumor-associated mesenchymal stem cells in chemically-induced breast cancer in Wistar ratsA. P. Lykov0A. V. Kabakov1N. A. Bondarenko2O. V. Poveshchenko3T. V. Rayter4O. V. Kazakov5D. N. Strunkin6M. A. Surovtseva7A. F. Poveshchenko8V. I. Konenkov9Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of Sciences; Research Institute of Circulation Pathology, Ministry of Health Care of Russian FederationResearch Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of SciencesResearch Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of Sciences; Research Institute of Circulation Pathology, Ministry of Health Care of Russian FederationResearch Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of Sciences; Research Institute of Circulation Pathology, Ministry of Health Care of Russian FederationResearch Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of SciencesResearch Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of SciencesResearch Institute of Circulation Pathology, Ministry of Health Care of Russian FederationResearch Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of Sciences; Research Institute of Circulation Pathology, Ministry of Health Care of Russian FederationResearch Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of Sciences; Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation; Research Institute of Fundamental and Clinical Immunology; Novosibirsk State Medical University; Novosibirsk State Pedagogic UniversityResearch Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics. Siberian Branch of Russian Academy of SciencesObjective: to compare the morphological and functional properties of mesenchymal stem cells from mammary tissues and chemically-induced mammary tumor tissues. material and methods. The study included 25 female Wistar rats. In 20 rats, mammary carcinoma was induced by intramammary injection of N-methyl-N-nitrosourea after estrus synchronization with chorionic gonadotropin. The control group consisted of 5 rats. Mammary carcinoma was verified histologically and immunohistochemically. To examine, whether the cells isolated from normal tissue and tumor tissue belonged to mesenchymal stem cells, FACS Canto II flow cytofluorometer was used. The functional properties of mesenchymal stem cells were evaluated in MTT assay by the level of nitric oxide production in normal and by hydrogen peroxide-induced hypoxia. The levels of prolactin, luteinizing hormone and estradiol E2 in urine were studied using solid-phase enzyme-linked immunosorbent assay. results. Chemically-induced mammary tumor according to histological and immunohistochemical studies corresponded to luminal B type breast cancer in humans. In rats that developed mammary tumors, the urine prolactin levels after synchronization of estrus were increased. In rats that did not develop tumors, the levels of prolactin and luteinizing hormone were decreased, but the levels of estradiol E2 were increased. More mesenchymal stem cells with CD45-/CD90+phenotype were obtained from the breast tumor tissue. Mesenchymal stem cells from tumor tissue showed increased proliferative potential and were more resistant to hypoxia. conclusion. Tumor- associated mesenchymal stem cells having high proliferative potential and resistance to hypoxia were obtained from chemically-induced mammary tumor tissue. Morphologic and functional differences in mesenchymal stem cells obtained from mammary breast tissue and tumor tissue require further studies.https://www.siboncoj.ru/jour/article/view/960breast tumormesenchymal stem cellsproliferationnitric oxidesex hormonesurine |
| spellingShingle | A. P. Lykov A. V. Kabakov N. A. Bondarenko O. V. Poveshchenko T. V. Rayter O. V. Kazakov D. N. Strunkin M. A. Surovtseva A. F. Poveshchenko V. I. Konenkov Tumor-associated mesenchymal stem cells in chemically-induced breast cancer in Wistar rats Сибирский онкологический журнал breast tumor mesenchymal stem cells proliferation nitric oxide sex hormones urine |
| title | Tumor-associated mesenchymal stem cells in chemically-induced breast cancer in Wistar rats |
| title_full | Tumor-associated mesenchymal stem cells in chemically-induced breast cancer in Wistar rats |
| title_fullStr | Tumor-associated mesenchymal stem cells in chemically-induced breast cancer in Wistar rats |
| title_full_unstemmed | Tumor-associated mesenchymal stem cells in chemically-induced breast cancer in Wistar rats |
| title_short | Tumor-associated mesenchymal stem cells in chemically-induced breast cancer in Wistar rats |
| title_sort | tumor associated mesenchymal stem cells in chemically induced breast cancer in wistar rats |
| topic | breast tumor mesenchymal stem cells proliferation nitric oxide sex hormones urine |
| url | https://www.siboncoj.ru/jour/article/view/960 |
| work_keys_str_mv | AT aplykov tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT avkabakov tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT nabondarenko tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT ovpoveshchenko tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT tvrayter tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT ovkazakov tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT dnstrunkin tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT masurovtseva tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT afpoveshchenko tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats AT vikonenkov tumorassociatedmesenchymalstemcellsinchemicallyinducedbreastcancerinwistarrats |